Literature DB >> 7528005

Synthesis and characterization of 3H-labelled tetrahydrobiopterin.

E R Werner1, M Schmid, G Werner-Felmayer, B Mayer, H Wachter.   

Abstract

We synthesized [3'-3H]-5,6,7,8-tetrahydrobiopterin from [8,5'-3H]guanosine 5'-triphosphate ([8,5'-3H]GTP) using GTP cyclohydrolase (EC 3.5.4.16), 6-pyruvoyltetrahydropterin synthase and sepiapterin reductase (EC 1.1.1.153). After purification by cation-exchange h.p.l.c. a solution of radiochemically pure (> 95%) [3'-3H]-5,6,7,8-tetrahydrobiopterin with a specific activity of 9.2 Ci/mmol was obtained. The product proved well suited for studying the binding of tetrahydrobiopterin to nitric-oxide synthase.

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Year:  1994        PMID： 7528005      PMCID： PMC1137470          DOI： 10.1042/bj3040189

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  20 in total

1.  Purification of a Ca2+/calmodulin-dependent nitric oxide synthase from porcine cerebellum. Cofactor-role of tetrahydrobiopterin.

Authors:  B Mayer; M John; E Böhme
Journal:  FEBS Lett       Date:  1990-12-17       Impact factor: 4.124

2.  Parallel induction of tetrahydrobiopterin biosynthesis and indoleamine 2,3-dioxygenase activity in human cells and cell lines by interferon-gamma.

Authors:  E R Werner; G Werner-Felmayer; D Fuchs; A Hausen; G Reibnegger; H Wachter
Journal:  Biochem J       Date:  1989-09-15       Impact factor: 3.857

3.  Purification and characterization of 6-pyruvoyl-tetrahydropterin synthase from Drosophila melanogaster.

Authors:  Y S Park; J H Kim; K B Jacobson; J J Yim
Journal:  Biochim Biophys Acta       Date:  1990-04-19

4.  Sepiapterin reductase from horse liver: purification and properties of the enzyme.

Authors:  S Kato
Journal:  Arch Biochem Biophys       Date:  1971-09       Impact factor: 4.013

Review 5.  Biosynthesis and metabolism of tetrahydrobiopterin and molybdopterin.

Authors:  C A Nichol; G K Smith; D S Duch
Journal:  Annu Rev Biochem       Date:  1985       Impact factor: 23.643

6.  Separation and properties of the 6-diastereoisomers of l-erythro-tetrahydrobiopterin and their reactivities with phenylalanine hydroxylase.

Authors:  S W Bailey; J E Ayling
Journal:  J Biol Chem       Date:  1978-03-10       Impact factor: 5.157

7.  Analysis of reduced forms of biopterin in biological tissues and fluids.

Authors:  T Fukushima; J C Nixon
Journal:  Anal Biochem       Date:  1980-02       Impact factor: 3.365

8.  Biopterin cofactor biosynthesis: independent regulation of GTP cyclohydrolase in adrenal medulla and cortex.

Authors:  O H Viveros; C L Lee; M M Abou-Donia; J C Nixon; C A Nichol
Journal:  Science       Date:  1981-07-17       Impact factor: 47.728

9.  The pteridine binding site of brain nitric oxide synthase. Tetrahydrobiopterin binding kinetics, specificity, and allosteric interaction with the substrate domain.

Authors:  P Klatt; M Schmid; E Leopold; K Schmidt; E R Werner; B Mayer
Journal:  J Biol Chem       Date:  1994-05-13       Impact factor: 5.157

10.  Tetrahydrobiopterin-dependent formation of nitrite and nitrate in murine fibroblasts.

Authors:  G Werner-Felmayer; E R Werner; D Fuchs; A Hausen; G Reibnegger; H Wachter
Journal:  J Exp Med       Date:  1990-12-01       Impact factor: 14.307
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  6 in total

1.  Contrasting effects of N5-substituted tetrahydrobiopterin derivatives on phenylalanine hydroxylase, dihydropteridine reductase and nitric oxide synthase.

Authors:  E R Werner; H J Habisch; A C Gorren; K Schmidt; L Canevari; G Werner-Felmayer; B Mayer
Journal:  Biochem J       Date:  2000-06-15       Impact factor: 3.857

2.  Characterization of bovine endothelial nitric oxide synthase as a homodimer with down-regulated uncoupled NADPH oxidase activity: tetrahydrobiopterin binding kinetics and role of haem in dimerization.

Authors:  B M List; B Klösch; C Völker; A C Gorren; W C Sessa; E R Werner; W R Kukovetz; K Schmidt; B Mayer
Journal:  Biochem J       Date:  1997-04-01       Impact factor: 3.857

3.  Haem insertion, dimerization and reactivation of haem-free rat neuronal nitric oxide synthase.

Authors:  B Hemmens; A C Gorren; K Schmidt; E R Werner; B Mayer
Journal:  Biochem J       Date:  1998-06-01       Impact factor: 3.857

4.  Overexpression of neuronal nitric oxide synthase in insect cells reveals requirement of haem for tetrahydrobiopterin binding.

Authors:  B M List; P Klatt; E R Werner; K Schmidt; B Mayer
Journal:  Biochem J       Date:  1996-04-01       Impact factor: 3.857

5.  Identification of the 4-amino analogue of tetrahydrobiopterin as a dihydropteridine reductase inhibitor and a potent pteridine antagonist of rat neuronal nitric oxide synthase.

Authors:  E R Werner; E Pitters; K Schmidt; H Wachter; G Werner-Felmayer; B Mayer
Journal:  Biochem J       Date:  1996-11-15       Impact factor: 3.857

6.  Interaction between neuronal nitric-oxide synthase and tetrahydrobiopterin revisited: studies on the nature and mechanism of tight pterin binding.

Authors:  Christian L Heine; Bernd Kolesnik; Renate Schmidt; Ernst R Werner; Bernd Mayer; Antonius C F Gorren
Journal:  Biochemistry       Date:  2014-02-17       Impact factor: 3.162
  6 in total

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