Literature DB >> 75241

Human migration inhibitory factor: purification and immunochemical characterization.

L H Block, H Jaksche, S Bamberger, G Ruhenstroth-Bauer.   

Abstract

Using gel filtration and preparative isotachophoresis, the migration inhibitory factor (MIF) was highly purified from human lymphocytes activated with concanavalin A. MIF is an acidic protein with a mol wt of approximately equal to 25,000 daltons as determined by gel filtration and analytical polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. The protein inhibits migration of macrophages in the capillary test and in addition, has a slowing effect on the electrophoretic mobility of guinea pig peritoneal macrophages. Rabbit antibodies specific for this protein, as determined by immunochemical techniques, neutralized the biological effect of MIF on migration and on the electrophoretic mobility of macrophages.

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Year:  1978        PMID： 75241      PMCID： PMC2184502          DOI： 10.1084/jem.147.2.541

Source DB:  PubMed          Journal:  J Exp Med        ISSN： 0022-1007            Impact factor:   14.307


  19 in total

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Authors:  E A CASPARY; E J FIELD
Journal:  Ann N Y Acad Sci       Date:  1965-03-31       Impact factor: 5.691

5.  Protein measurement with the Folin phenol reagent.

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Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

6.  Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g.

Authors:  A Böyum
Journal:  Scand J Clin Lab Invest Suppl       Date:  1968

7.  Macrophage electrophoretic mobility (MEM) test in cancer: a critical evaluation.

Authors:  E J Field; E A Caspary; K S Smith
Journal:  Br J Cancer Suppl       Date:  1973-08

8.  The macrophage electrophoretic mobility (MEM) test for malignant disease. Further clinical investigations and studies on macrophage slowing factors.

Authors:  A W Preece; P A Light
Journal:  Clin Exp Immunol       Date:  1974-12       Impact factor: 4.330

9.  The production of heterologous antibodies to the human lymphakine: leukocyte migration inhibitory factor (LIF).

Authors:  K Bendtzen
Journal:  Cell Immunol       Date:  1977-03-15       Impact factor: 4.868

10.  Studies on migration inhibitory factor (MIF): recovery of MIF activity after purification by gel filtration and disc electrophoresis.

Authors:  H G Remold; A B Katz; E Haber; J R David
Journal:  Cell Immunol       Date:  1970-05       Impact factor: 4.868
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  10 in total

1.  [Immunopharmacology of corticosteroids].

Authors:  L H Block; W Vetter; W Siegenthaler
Journal:  Klin Wochenschr       Date:  1982-11-15

Review 2.  The role of lymphokines in delayed-type hypersensitivity reactions.

Authors:  C L Geczy
Journal:  Springer Semin Immunopathol       Date:  1984

3.  Purification of guinea pig pH 3 migration inhibitory factor.

Authors:  H G Remold; P L McCarthy; A D Mednis
Journal:  Proc Natl Acad Sci U S A       Date:  1981-07       Impact factor: 11.205

4.  Isolation and characterization of an RNA-proteolipid complex associated with the malignant state in humans.

Authors:  A J Wieczorek; C Rhyner; L H Block
Journal:  Proc Natl Acad Sci U S A       Date:  1985-05       Impact factor: 11.205

5.  Molecular cloning of a cDNA encoding a human macrophage migration inhibitory factor.

Authors:  W Y Weiser; P A Temple; J S Witek-Giannotti; H G Remold; S C Clark; J R David
Journal:  Proc Natl Acad Sci U S A       Date:  1989-10       Impact factor: 11.205

6.  Biological and clinical relevance of human macrophage migration inhibitory factor (MIF).

Authors:  L H Block; G Ruhenstroth-Bauer
Journal:  Blut       Date:  1979-02-19

7.  Inhibition of mitogen-induced lymphokine production by cyclosporin A.

Authors:  L H Block; W Siegenthaler; J Drews
Journal:  Klin Wochenschr       Date:  1980-07-15

8.  Flow-cytometric measurements of the transmembrane potential, the surface charge density and the phagocytic activity of Guinea pig macrophages after incubation with lymphokines.

Authors:  G Valet; H L Jenssen; M Krefft; G Ruhenstroth-Bauer
Journal:  Blut       Date:  1981-06

9.  Production of migration inhibition factor (MIF) by purified human T cell subpopulations.

Authors:  R J Warrington; S L Olivier; P J Sauder; W J Rutherford
Journal:  Clin Exp Immunol       Date:  1981-05       Impact factor: 4.330

10.  Activation of macrophages in vivo and in vitro. Correlation between hydrogen peroxide release and killing of Trypanosoma cruzi.

Authors:  C Nathan; N Nogueira; C Juangbhanich; J Ellis; Z Cohn
Journal:  J Exp Med       Date:  1979-05-01       Impact factor: 14.307
  10 in total

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