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Literature DB >> 7515186

Three-dimensional working model of M1 RNA, the catalytic RNA subunit of ribonuclease P from Escherichia coli.

Abstract

A three-dimensional model of M1 RNA, the catalytic RNA subunit of RNase P from Escherichia coli, was constructed with the aid of a computer. The modeling process took into account data from chemical and enzymatic protection experiments, phylogenetic analysis, studies of the activities of mutants, and the kinetics of reactions catalyzed by the binding of substrate to M1 RNA. The model provides a plausible picture of the binding to M1 RNA of the tRNA domain of a precursor tRNA substrate. The scissile bond and adjacent segments of the aminoacyl acceptor stem of a precursor tRNA substrate can fit into a cleft that leads to the phylogenetically conserved, central part of the structure.

Entities: Chemical Gene Species

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Year: 1994 PMID： 7515186 PMCID： PMC43946 DOI： 10.1073/pnas.91.11.5133

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

35 in total

9. Purification and characterization of the nuclear RNase P holoenzyme complex reveals extensive subunit overlap with RNase MRP.

Authors: J R Chamberlain; Y Lee; W S Lane; D R Engelke
Journal: Genes Dev Date: 1998-06-01 Impact factor: 11.361

10. Identification by modification-interference of purine N-7 and ribose 2'-OH groups critical for catalysis by bacterial ribonuclease P.

Authors: A V Kazantsev; N R Pace
Journal: RNA Date: 1998-08 Impact factor: 4.942

Three-dimensional working model of M1 RNA, the catalytic RNA subunit of ribonuclease P from Escherichia coli.

1. Solution structure of a tRNA with a large variable region: yeast tRNASer.

2. Involvement of a GNRA tetraloop in long-range RNA tertiary interactions.

3. Selection and characterization of randomly produced mutants in the gene coding for M1 RNA.

4. Specific interactions in RNA enzyme-substrate complexes.

5. Computer modeling from solution data of spinach chloroplast and of Xenopus laevis somatic and oocyte 5 S rRNAs.

6. Model for the three-dimensional folding of 16 S ribosomal RNA.

7. Structural conventions for group I introns.

8. Crystallographic refinement of yeast aspartic acid transfer RNA.

9. Structure of E. coli glutaminyl-tRNA synthetase complexed with tRNA(Gln) and ATP at 2.8 A resolution.

10. Functional domains of the RNA component of ribonuclease P revealed by chemical probing of mutant RNAs.

1. UV cross-link mapping of the substrate-binding site of an RNase P ribozyme to a target mRNA sequence.

2. New insight into RNase P RNA structure from comparative analysis of the archaeal RNA.

3. RNA aptamers that specifically bind to a 16S ribosomal RNA decoding region construct.

4. The first phytoplasma RNase P RNA provides new insights into the sequence requirements of this ribozyme.

5. Heuristic RNA pseudoknot prediction including intramolecular kissing hairpins.

Review 6. Evolutionary perspective on the structure and function of ribonuclease P, a ribozyme.

7. Multiple substrate binding sites in the ribozyme from Bacillus subtilis RNase P.

8. Coarse-grained modeling of large RNA molecules with knowledge-based potentials and structural filters.

9. Purification and characterization of the nuclear RNase P holoenzyme complex reveals extensive subunit overlap with RNase MRP.

10. Identification by modification-interference of purine N-7 and ribose 2'-OH groups critical for catalysis by bacterial ribonuclease P.