Literature DB >> 7487013

Identification of algae which interfere with the detection of Giardia cysts and Cryptosporidium oocysts and a method for alleviating this interference.

M R Rodgers1, D J Flanigan, W Jakubowski.   

Abstract

Fifty-four algal species were tested for cross-reaction in the American Society for Testing and Materials Giardia/Cryptosporidium indirect immunofluorescence assay, and 24 showed some degree of fluorescence. Two species, Navicula minima and Synechococcus elongatus, exhibited a bright apple green fluorescence. The addition of goat serum to the assay mixture blocked the fluorescence of most nontarget organisms tested and also decreased the background fluorescence. Goat serum did not interfere with the fluorescence of Giardia cysts or Cryptosporidium oocysts or the identification of cyst and oocyst internal structures.

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Year:  1995        PMID： 7487013      PMCID： PMC167676          DOI： 10.1128/aem.61.10.3759-3763.1995

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  8 in total

1.  The experimental transmission of human intestinal protozoan parasites. II. Giardia lamblia cysts given in capsules.

Authors:  R C RENDTORFF
Journal:  Am J Hyg       Date:  1954-03

2.  Survival of Cryptosporidium parvum oocysts under various environmental pressures.

Authors:  L J Robertson; A T Campbell; H V Smith
Journal:  Appl Environ Microbiol       Date:  1992-11       Impact factor: 4.792

3.  Giardia sp.: physical factors of excystation in vitro, and excystation vs eosin exclusion as determinants of viability.

Authors:  A K Bingham; E L Jarroll; E A Meyer; S Radulescu
Journal:  Exp Parasitol       Date:  1979-04       Impact factor: 2.011

4.  "Protein A" from S. aureus. I. Pseudo-immune reaction with human gamma-globulin.

Authors:  A Forsgren; J Sjöquist
Journal:  J Immunol       Date:  1966-12       Impact factor: 5.422

5.  The infectivity of Cryptosporidium parvum in healthy volunteers.

Authors:  H L DuPont; C L Chappell; C R Sterling; P C Okhuysen; J B Rose; W Jakubowski
Journal:  N Engl J Med       Date:  1995-03-30       Impact factor: 91.245

6.  Viability of Giardia cysts suspended in lake, river, and tap water.

Authors:  D P deRegnier; L Cole; D G Schupp; S L Erlandsen
Journal:  Appl Environ Microbiol       Date:  1989-05       Impact factor: 4.792

7.  A massive outbreak in Milwaukee of cryptosporidium infection transmitted through the public water supply.

Authors:  W R Mac Kenzie; N J Hoxie; M E Proctor; M S Gradus; K A Blair; D E Peterson; J J Kazmierczak; D G Addiss; K R Fox; J B Rose
Journal:  N Engl J Med       Date:  1994-07-21       Impact factor: 91.245

8.  Occurrence of Giardia and Cryptosporidium spp. in surface water supplies.

Authors:  M W LeChevallier; W D Norton; R G Lee
Journal:  Appl Environ Microbiol       Date:  1991-09       Impact factor: 4.792
  8 in total
  14 in total

1.  Computer-Assisted Laser Scanning and Video Microscopy for Analysis of Cryptosporidium parvum Oocysts in Soil, Sediment, and Feces.

Authors:  L J Anguish; W C Ghiorse
Journal:  Appl Environ Microbiol       Date:  1997-02       Impact factor: 4.792

Review 2.  Flow cytometry and cell sorting of heterogeneous microbial populations: the importance of single-cell analyses.

Authors:  H M Davey; D B Kell
Journal:  Microbiol Rev       Date:  1996-12

3.  Assessment of Giardia and Cryptosporidium spp. as a microbial source tracking tool for surface water: application in a mixed-use watershed.

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4.  Differences in staining intensities affect reported occurrences and concentrations of Giardia spp. in surface drinking water sources.

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5.  Sensitive and rapid detection of viable Giardia cysts and Cryptosporidium parvum oocysts in large-volume water samples with wound fiberglass cartridge filters and reverse transcription-PCR.

Authors:  C Kaucner; T Stinear
Journal:  Appl Environ Microbiol       Date:  1998-05       Impact factor: 4.792

6.  Evaluation of PCR, nested PCR, and fluorescent antibodies for detection of Giardia and Cryptosporidium species in wastewater.

Authors:  C L Mayer; C J Palmer
Journal:  Appl Environ Microbiol       Date:  1996-06       Impact factor: 4.792

Review 7.  Waterborne protozoan pathogens.

Authors:  M M Marshall; D Naumovitz; Y Ortega; C R Sterling
Journal:  Clin Microbiol Rev       Date:  1997-01       Impact factor: 26.132

8.  Identification of Cryptosporidium parvum oocysts by an artificial neural network approach.

Authors:  Kenneth W Widmer; Kevin H Oshima; Suresh D Pillai
Journal:  Appl Environ Microbiol       Date:  2002-03       Impact factor: 4.792

9.  Cryptosporidium oocyst detection in water samples: floatation technique enhanced with immunofluorescence is as effective as immunomagnetic separation method.

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Journal:  Korean J Parasitol       Date:  2009-12-01       Impact factor: 1.341

Review 10.  Epidemiology and detection as options for control of viral and parasitic foodborne disease.

Authors:  L A Jaykus
Journal:  Emerg Infect Dis       Date:  1997 Oct-Dec       Impact factor: 6.883
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