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Literature DB >> 7487010

A new method for direct detection of Listeria monocytogenes from foods by PCR.

Abstract

The preparation of DNA by alcohol precipitation in the presence of Na1 was used to enable the direct detection of Listeria monocytogenes in the amount of 10(3) CFU/0.5 g of sample. This procedure produces PCR-quality DNA directly from foods, such as soft cheese and minced meat.

Entities: Chemical Species

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Year: 1995 PMID： 7487010 PMCID： PMC167673 DOI： 10.1128/aem.61.10.3745-3747.1995

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

7 in total

1. Simple procedure of DNA isolation from human serum.

Authors: M Ishizawa; Y Kobayashi; T Miyamura; S Matsuura
Journal: Nucleic Acids Res Date: 1991-10-25 Impact factor: 16.971

2. Enhanced sensitivity in PCR detection of Listeria monocytogenes in soft cheese through use of an aqueous two-phase system as a sample preparation method.

Authors: P G Lantz; F Tjerneld; E Borch; B Hahn-Hägerdal; P Rådström
Journal: Appl Environ Microbiol Date: 1994-09 Impact factor: 4.792

3. Direct detection of Bacillus anthracis DNA in animals by polymerase chain reaction.

Authors: S I Makino; Y Iinuma-Okada; T Maruyama; T Ezaki; C Sasakawa; M Yoshikawa
Journal: J Clin Microbiol Date: 1993-03 Impact factor: 5.948

Review 4. Listeria monocytogenes, a food-borne pathogen.

Authors: J M Farber; P I Peterkin
Journal: Microbiol Rev Date: 1991-09

5. Use of the polymerase chain reaction for direct detection of Listeria monocytogenes in soft cheese.

Authors: K Wernars; C J Heuvelman; T Chakraborty; S H Notermans
Journal: J Appl Bacteriol Date: 1991-02

6. Detection of Listeria monocytogenes in cheese with the magnetic immuno-polymerase chain reaction assay.

Authors: A C Fluit; R Torensma; M J Visser; C J Aarsman; M J Poppelier; B H Keller; P Klapwijk; J Verhoef
Journal: Appl Environ Microbiol Date: 1993-05 Impact factor: 4.792

7. Direct and rapid detection of Erysipelothrix rhusiopathiae DNA in animals by PCR.

Authors: S Makino; Y Okada; T Maruyama; K Ishikawa; T Takahashi; M Nakamura; T Ezaki; H Morita
Journal: J Clin Microbiol Date: 1994-06 Impact factor: 5.948

7 in total

1. Direct and rapid detection by PCR of Erysipelothrix sp. DNAs prepared from bacterial strains and animal tissues.

Authors: K Takeshi; S Makino; T Ikeda; N Takada; A Nakashiro; K Nakanishi; K Oguma; Y Katoh; H Sunagawa; T Ohyama
Journal: J Clin Microbiol Date: 1999-12 Impact factor: 5.948

2. Inhibitory effects of collagen on the PCR for detection of Clostridium perfringens.

Authors: S Kim; R G Labbe; S Ryu
Journal: Appl Environ Microbiol Date: 2000-03 Impact factor: 4.792

3. Detection of Salmonella spp. and Listeria monocytogenes in suspended organic waste by nucleic acid extraction and PCR.

Authors: C Burtscher; P A Fall; P A Wilderer; S Wuertz
Journal: Appl Environ Microbiol Date: 1999-05 Impact factor: 4.792

7. Evaluation of the use of PCR and reverse transcriptase PCR for detection of pathogenic bacteria in biosolids from anaerobic digestors and aerobic composters.

Authors: Carola Burtscher; Stefan Wuertz
Journal: Appl Environ Microbiol Date: 2003-08 Impact factor: 4.792

7 in total

A new method for direct detection of Listeria monocytogenes from foods by PCR.

1. Simple procedure of DNA isolation from human serum.

2. Enhanced sensitivity in PCR detection of Listeria monocytogenes in soft cheese through use of an aqueous two-phase system as a sample preparation method.

3. Direct detection of Bacillus anthracis DNA in animals by polymerase chain reaction.

Review 4. Listeria monocytogenes, a food-borne pathogen.

5. Use of the polymerase chain reaction for direct detection of Listeria monocytogenes in soft cheese.

6. Detection of Listeria monocytogenes in cheese with the magnetic immuno-polymerase chain reaction assay.

7. Direct and rapid detection of Erysipelothrix rhusiopathiae DNA in animals by PCR.

1. Direct and rapid detection by PCR of Erysipelothrix sp. DNAs prepared from bacterial strains and animal tissues.

2. Inhibitory effects of collagen on the PCR for detection of Clostridium perfringens.

3. Detection of Salmonella spp. and Listeria monocytogenes in suspended organic waste by nucleic acid extraction and PCR.

Review 4. Inhibition and facilitation of nucleic acid amplification.

5. Detection and identification of Listeria monocytogenes from milk and cheese by a single-step PCR.

6. Sensitive detection of viable Listeria monocytogenes by reverse transcription-PCR.

7. Evaluation of the use of PCR and reverse transcriptase PCR for detection of pathogenic bacteria in biosolids from anaerobic digestors and aerobic composters.