Literature DB >> 7524445

Enhanced sensitivity in PCR detection of Listeria monocytogenes in soft cheese through use of an aqueous two-phase system as a sample preparation method.

P G Lantz1, F Tjerneld, E Borch, B Hahn-Hägerdal, P Rådström.   

Abstract

A sample treatment method based on an aqueous two-phase system containing polyethylene glycol and dextran was developed for enhancing sensitivity in the detection of Listeria monocytogenes in soft cheese with PCR. The results suggest that the improved detection sensitivity following partitioning of the cheese homogenate in an aqueous two-phase system may be due to partitioning of the PCR inhibitors to the polyethylene glycol phase.

Entities:  

Mesh：

Substances：

Year:  1994        PMID： 7524445      PMCID： PMC201820          DOI： 10.1128/aem.60.9.3416-3418.1994

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  7 in total

1.  Direct extraction of bacterial plasmids from food for polymerase chain reaction amplification.

Authors:  M R Andersen; C J Omiecinski
Journal:  Appl Environ Microbiol       Date:  1992-12       Impact factor: 4.792

2.  On the evolutionary descent of organisms and organelles: a global phylogeny based on a highly conserved structural core in small subunit ribosomal RNA.

Authors:  M W Gray; D Sankoff; R J Cedergren
Journal:  Nucleic Acids Res       Date:  1984-07-25       Impact factor: 16.971

3.  Phylogenetic analysis of the genus Listeria based on reverse transcriptase sequencing of 16S rRNA.

Authors:  M D Collins; S Wallbanks; D J Lane; J Shah; R Nietupski; J Smida; M Dorsch; E Stackebrandt
Journal:  Int J Syst Bacteriol       Date:  1991-04

4.  Rapid method for separation of bacterial DNA from humic substances in sediments for polymerase chain reaction.

Authors:  Y L Tsai; B H Olson
Journal:  Appl Environ Microbiol       Date:  1992-07       Impact factor: 4.792

5.  Inhibition of PCR by components of food samples, microbial diagnostic assays and DNA-extraction solutions.

Authors:  L Rossen; P Nørskov; K Holmstrøm; O F Rasmussen
Journal:  Int J Food Microbiol       Date:  1992-09       Impact factor: 5.277

6.  Use of the polymerase chain reaction for direct detection of Listeria monocytogenes in soft cheese.

Authors:  K Wernars; C J Heuvelman; T Chakraborty; S H Notermans
Journal:  J Appl Bacteriol       Date:  1991-02

7.  Detection of Listeria monocytogenes in cheese with the magnetic immuno-polymerase chain reaction assay.

Authors:  A C Fluit; R Torensma; M J Visser; C J Aarsman; M J Poppelier; B H Keller; P Klapwijk; J Verhoef
Journal:  Appl Environ Microbiol       Date:  1993-05       Impact factor: 4.792
  7 in total
  13 in total

1.  Purification and characterization of PCR-inhibitory components in blood cells.

Authors:  W A Al-Soud; P Rådström
Journal:  J Clin Microbiol       Date:  2001-02       Impact factor: 5.948

2.  Effects of amplification facilitators on diagnostic PCR in the presence of blood, feces, and meat.

Authors:  W Abu Al-Soud; P Rådström
Journal:  J Clin Microbiol       Date:  2000-12       Impact factor: 5.948

Review 3.  Pre-PCR processing: strategies to generate PCR-compatible samples.

Authors:  Peter Rådström; Rickard Knutsson; Petra Wolffs; Maria Lövenklev; Charlotta Löfström
Journal:  Mol Biotechnol       Date:  2004-02       Impact factor: 2.695

Review 4.  Inhibition and facilitation of nucleic acid amplification.

Authors:  I G Wilson
Journal:  Appl Environ Microbiol       Date:  1997-10       Impact factor: 4.792

5.  Application of a 5' nuclease assay for detection of Lawsonia intracellularis in fecal samples from pigs.

Authors:  R H Lindecrona; T K Jensen; P H Andersen; K Møller
Journal:  J Clin Microbiol       Date:  2002-03       Impact factor: 5.948

6.  Identification and characterization of immunoglobulin G in blood as a major inhibitor of diagnostic PCR.

Authors:  W A Al-Soud; L J Jönsson; P Râdström
Journal:  J Clin Microbiol       Date:  2000-01       Impact factor: 5.948

7.  A new method for direct detection of Listeria monocytogenes from foods by PCR.

Authors:  S Makino; Y Okada; T Maruyama
Journal:  Appl Environ Microbiol       Date:  1995-10       Impact factor: 4.792

8.  Immobilization with metal hydroxides as a means to concentrate food-borne bacteria for detection by cultural and molecular methods.

Authors:  L A Lucore; M A Cullison; L A Jaykus
Journal:  Appl Environ Microbiol       Date:  2000-05       Impact factor: 4.792

9.  Capacity of nine thermostable DNA polymerases To mediate DNA amplification in the presence of PCR-inhibiting samples.

Authors:  W Abu Al-Soud; P Râdström
Journal:  Appl Environ Microbiol       Date:  1998-10       Impact factor: 4.792

10.  Identification of Clostridium tyrobutyricum as the causative agent of late blowing in cheese by species-specific PCR amplification.

Authors:  N Klijn; F F Nieuwenhof; J D Hoolwerf; C B van der Waals; A H Weerkamp
Journal:  Appl Environ Microbiol       Date:  1995-08       Impact factor: 4.792
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.