Literature DB >> 10698795

Inhibitory effects of collagen on the PCR for detection of Clostridium perfringens.

S Kim1, R G Labbe, S Ryu.   

Abstract

It is essential to identify specific food components that inhibit PCR in order to increase the sensitivity of the PCR method for rapid detection of pathogens contaminating a food. We found that collagen, a major component of several foods, inhibited PCR. The inhibitory action of collagen on PCR could be partially reversed by adjusting the concentration of magnesium ion in the reaction mixture and by the use of various DNA extraction methods to remove the collagen from the DNA. Also, the source of thermostable DNA polymerase was affected by the presence of collagen. These results suggest the need to optimize the extraction and assay conditions for rapid detection of enterotoxigenic Clostridium perfringens by PCR with respect to the kind of food being analyzed.

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Year:  2000        PMID： 10698795      PMCID： PMC91966          DOI： 10.1128/AEM.66.3.1213-1215.2000

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  15 in total

1.  The effects of plant polysaccharides and buffer additives on PCR.

Authors:  T Demeke; R P Adams
Journal:  Biotechniques       Date:  1992-03       Impact factor: 1.993

2.  PCR based diagnosis in the presence of 8% (v/v) blood.

Authors:  M Panaccio; A Lew
Journal:  Nucleic Acids Res       Date:  1991-03-11       Impact factor: 16.971

3.  A simple technique for removing plant polysaccharide contaminants from DNA.

Authors:  N Do; R P Adams
Journal:  Biotechniques       Date:  1991-02       Impact factor: 1.993

Review 4.  Clostridium perfringens toxins (type A, B, C, D, E).

Authors:  J L McDonel
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5.  Rapid method for separation of bacterial DNA from humic substances in sediments for polymerase chain reaction.

Authors:  Y L Tsai; B H Olson
Journal:  Appl Environ Microbiol       Date:  1992-07       Impact factor: 4.792

6.  Inhibition of PCR by components of food samples, microbial diagnostic assays and DNA-extraction solutions.

Authors:  L Rossen; P Nørskov; K Holmstrøm; O F Rasmussen
Journal:  Int J Food Microbiol       Date:  1992-09       Impact factor: 5.277

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Journal:  J Clin Microbiol       Date:  1993-03       Impact factor: 5.948

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Authors:  K Wernars; C J Heuvelman; T Chakraborty; S H Notermans
Journal:  J Appl Bacteriol       Date:  1991-02

9.  Comparison of Western immunoblots and gene detection assays for identification of potentially enterotoxigenic isolates of Clostridium perfringens.

Authors:  J F Kokai-Kun; J G Songer; J R Czeczulin; F Chen; B A McClane
Journal:  J Clin Microbiol       Date:  1994-10       Impact factor: 5.948

10.  Capacity of nine thermostable DNA polymerases To mediate DNA amplification in the presence of PCR-inhibiting samples.

Authors:  W Abu Al-Soud; P Râdström
Journal:  Appl Environ Microbiol       Date:  1998-10       Impact factor: 4.792
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  10 in total

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