Literature DB >> 461181

Rapid synthesis of oligodeoxyribonucleotides on a grafted polymer support.

V K Potapov, V P Veiko, O N Koroleva, Z A Shabarova.   

Abstract

Rapid synthesis of oligodeoxyribonucleotides is described by a solid phase method. Polysterene grafted on the surface of polytetrafluoroethylene has been found to be an adequate support. The properties of the support were studied in the synthesis of a number of oligonucleotides 6 to 15 nucleotides long. A flow-type semi-automatic synthesizer has been used. Each nucleotide addition involves seven steps and takes 8 hours. Oligonucleotide isolated and purified by ion-exchange chromatography amounts to 1-3 mumol per gram of support.

Entities:  

Mesh：

Substances：

Year:  1979        PMID： 461181      PMCID： PMC327835          DOI： 10.1093/nar/6.6.2041

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  9 in total

1.  THE EFFECT OF UREA, FORMAMIDE, AND GLYCOLS ON THE SECONDARY BINDING FORCES IN THE ION-EXCHANGE CHROMATOGRAPHY OF POLYNUCLEOTIDES OF DEAE-CELLULOSE.

Authors:  R V TOMLINSON; G M TENER
Journal:  Biochemistry       Date:  1963 Jul-Aug       Impact factor: 3.162

2.  CV. Total synthesis of the structural gene for an alanine transfer ribonucleic acid from yeast. Chemical synthesis of an icosadeoxyribonucleotide corresponding to the nucleotide sequence 31 to 50.

Authors:  H Büchi; H G Khorana
Journal:  J Mol Biol       Date:  1972-12-28       Impact factor: 5.469

3.  CIV. Total synthesis of the structural gene for an alanine transfer ribonucleic acid from yeast. Chemical synthesis of an icosadeoxynucleotide corresponding to the nucleotide sequence 21 to 40.

Authors:  H Weber; H G Khorana
Journal:  J Mol Biol       Date:  1972-12-28       Impact factor: 5.469

4.  Chromatography of 32P-labelled oligonucleotides on thin layers of DEAE-cellulose.

Authors:  G G Brownlee; F Sanger
Journal:  Eur J Biochem       Date:  1969-12

5.  Studies on transfer ribonucleic acids and related compounds. V. Synthesis of ribonucleotides with phosphomonoester end groups on a polymer support.

Authors:  E Ohtsuka; S Morioka; M Ikehara
Journal:  J Am Chem Soc       Date:  1972-05-03       Impact factor: 15.419

6.  Studies on polynucleotides. 93. A further study of the synthesis of deoxyribopolynucleotides using preformed oligonucleotide blocks.

Authors:  J Hachmann; H G Khorana
Journal:  J Am Chem Soc       Date:  1969-05-07       Impact factor: 15.419

7.  Rous sarcoma virus genome is terminally redundant: the 5' sequence.

Authors:  W A Haseltine; A M Maxam; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1977-03       Impact factor: 11.205

8.  Rapid synthesis of oligodeoxyribonucleotides: a new solid-phase method.

Authors:  M J Gait; R C Sheppard
Journal:  Nucleic Acids Res       Date:  1977-04       Impact factor: 16.971

9.  Rapid synthesis of oligodeoxyribonucleotides. II. Machine-aided solid-phase syntheses of two nonanucleotides and an octanucleotide.

Authors:  M J Gait; R C Sheppard
Journal:  Nucleic Acids Res       Date:  1977-12       Impact factor: 16.971
  9 in total
  3 in total

1.  Synthesis of oligonucleotides on cellulose by a phosphotriester method.

Authors:  R Crea; T Horn
Journal:  Nucleic Acids Res       Date:  1980-05-24       Impact factor: 16.971

2.  Solid phase phosphotriester synthesis of large oligodeoxyribonucleotides on a polyamide support.

Authors:  A F Markham; M D Edge; T C Atkinson; A R Greene; G R Heathcliffe; C R Newton; D Scanlon
Journal:  Nucleic Acids Res       Date:  1980-11-25       Impact factor: 16.971

3.  Solid-phase synthesis of polynucleotides. IV. Usage of polystyrene resins for the synthesis of polydeoxyribonucleotides by the phosphostriester method.

Authors:  K Miyoshi; R Arentzen; T Huang; K Itakura
Journal:  Nucleic Acids Res       Date:  1980-11-25       Impact factor: 16.971
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.