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Literature DB >> 745991

DNA adjacent to attachment points of deoxyribonucleoprotein fibril to chromosomal axial structure is enriched in reiterated base sequences.

Abstract

Mitotic chromosomes of L cells (metaphase plates) were dehistonized by centrifugation through a layer of 2 M NaCl and then treated with restriction endonuclease Bam HI. Alternatively, they were pretreated with EcoRI endonuclease, dehistonized, and additionally digested with EcoRI or HindIII. The DNA remaining attached to the axial structure of the chromosomes was isolated and investigated in renaturation experiments. It was found to be enriched in reiterated base sequences belonging to the satellite and to abundant intermediate repeats. The CsCl density gradient ultracentrifugation of this DNA separated the satellite from the fraction containing intermediate repeats.

Entities: Chemical

Mesh：

Substances：

Year: 1978 PMID： 745991 PMCID： PMC342785 DOI： 10.1093/nar/5.12.4737

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

8 in total

1. Prepartation of soluble chromatin and specific chromatin fractions with restriction nucleases.

Authors: T Igo-Kemenes; W Greil; H G Zachau
Journal: Nucleic Acids Res Date: 1977-10 Impact factor: 16.971

2. Role of nonhistone proteins in metaphase chromosome structure.

Authors: K W Adolph; S M Cheng; U K Laemmli
Journal: Cell Date: 1977-11 Impact factor: 41.582

3. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors: P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal: J Mol Biol Date: 1977-06-15 Impact factor: 5.469

4. Metaphase chromosome structure: the role of nonhistone proteins.

Authors: U K Laemmli; S M Cheng; K W Adolph; J R Paulson; J A Brown; W R Baumbach
Journal: Cold Spring Harb Symp Quant Biol Date: 1978

5. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.

Authors: P A Sharp; B Sugden; J Sambrook
Journal: Biochemistry Date: 1973-07-31 Impact factor: 3.162

6. Very long stretches of free DNA in chromatin.

Authors: A J Varshavsky; Y V Ilyin; G P Georgiev
Journal: Nature Date: 1974-08-16 Impact factor: 49.962

7. Analysis of repeating DNA sequences by reassociation.

Authors: R J Britten; D E Graham; B R Neufeld
Journal: Methods Enzymol Date: 1974 Impact factor: 1.600

8. Isolation, characterization, and structure of the folded interphase genome of Drosophila melanogaster.

Authors: C Benyajati; A Worcel
Journal: Cell Date: 1976-11 Impact factor: 41.582

8 in total

12 in total

10. A partial characterization of DNA fragments protected from nuclease degradation in histone depleted metaphase chromosomes of the Chinese hamster.

Authors: P G Jeppesen; A T Bankier
Journal: Nucleic Acids Res Date: 1979-09-11 Impact factor: 16.971

DNA adjacent to attachment points of deoxyribonucleoprotein fibril to chromosomal axial structure is enriched in reiterated base sequences.

1. Prepartation of soluble chromatin and specific chromatin fractions with restriction nucleases.

2. Role of nonhistone proteins in metaphase chromosome structure.

3. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

4. Metaphase chromosome structure: the role of nonhistone proteins.

5. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.

6. Very long stretches of free DNA in chromatin.

7. Analysis of repeating DNA sequences by reassociation.

8. Isolation, characterization, and structure of the folded interphase genome of Drosophila melanogaster.

Review 1. A requiem to the nuclear matrix: from a controversial concept to 3D organization of the nucleus.

2. Functional role of a highly repetitive DNA sequence in anchorage of the mouse genome.

3. Attachment of repeated sequences to the nuclear cage.

4. The structure of partly decondensed metaphase chromosomes.

5. DNA fragments associated with chromosome scaffolds.

Review 6. Studies on structure and function of chromatin.

7. Granules 25-30 nm in diameter: basic constituent of the nuclear matrix, chromosome scaffold, and nuclear envelope.

8. Nonrandom distribution of repeated DNA sequences with respect to supercoiled loops and the nuclear matrix.

9. DNA-binding activity of tightly-bound nonhistone chromosomal proteins in chicken liver chromatin.

10. A partial characterization of DNA fragments protected from nuclease degradation in histone depleted metaphase chromosomes of the Chinese hamster.