Literature DB >> 7442662

R-loop hybridization of collagen DNA: separation in Cs2SO4 gradients.

J Born, B Wittig, S Wittig, H Tiedemann.   

Abstract

High molecular weight poly(A)RNA (26-35 S) from chicken embryo trunks which is enriched for collagen mRNA was iodinated and hybridized to DNA under conditions of R-loop formation. The R-loops were separated in Cs2SO4 gradients from the bulk of DNA yielding double stranded DNA enriched for collagen genes.

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Year:  1980        PMID： 7442662     DOI： 10.1007/bf00775410

Source DB:  PubMed          Journal:  Mol Biol Rep        ISSN： 0301-4851            Impact factor:   2.316


  20 in total

1.  Study of differential collagen synthesis during development of the chick embryo by immunofluorescence. I. Preparation of collagen type I and type II specific antibodies and their application to early stages of the chick embryo.

Authors:  H von der Mark; K von der Mark; S Gay
Journal:  Dev Biol       Date:  1976-02       Impact factor: 3.582

2.  Levels of translatable mRNAs for cell surface protein, collagen precursors, and two membrane proteins are altered in Rous sarcoma virus-transformed chick embryo fibroblasts.

Authors:  S L Adams; M E Sobel; B H Howard; K Olden; K M Yamada; B de Crombrugghe; I Pastan
Journal:  Proc Natl Acad Sci U S A       Date:  1977-08       Impact factor: 11.205

3.  Mechanisms of cell differentiation: Affinity of a morphogenetic factor to DNA.

Authors:  Heinz Tiedemann; Jochen Born; Hildegard Tiedemann
Journal:  Wilhelm Roux Arch Entwickl Mech Org       Date:  1972-06

4.  Improved methods for the formation and stabilization of R-loops.

Authors:  D B Kaback; L M Angerer; N Davidson
Journal:  Nucleic Acids Res       Date:  1979-06-11       Impact factor: 16.971

5.  Procollagen alpha2 mRNA is significantly different from procollagen alpha1(I) mRNA in size or secondary structure.

Authors:  P Tolstoshev; R Haber; R G Crystal
Journal:  Biochem Biophys Res Commun       Date:  1979-04-13       Impact factor: 3.575

Review 6.  Biosynthesis of collagen. The biochemist's view.

Authors:  E J Miller; V J Matukas
Journal:  Fed Proc       Date:  1974-05

7.  The synthesis of presumptive procollagen messenger ribonucleic acid in the calvaria of the developing chick embryo.

Authors:  D Breitkreutz; L Diaz de Leon; L Paglia; M Zeichner; J Wilczek; R Stern
Journal:  Biochim Biophys Acta       Date:  1978-02-16

8.  The localization and synthesis of some collagen types in developing mouse embryos.

Authors:  E D Adamson; S E Ayers
Journal:  Cell       Date:  1979-04       Impact factor: 41.582

9.  Chicken ribosomal DNA: gene frequency and purification by R-loop hybridization.

Authors:  C Lehmann; M Warnhoff; W Knochel; D Lange; J Born; H Tiedemann
Journal:  Mol Biol Rep       Date:  1979-02-15       Impact factor: 2.316

10.  Procollagen complementary DNA, a probe for messenger RNA purification and the number of type I collagen genes.

Authors:  A M Frischauf; H Lehrach; C Rosner; H Boedtker
Journal:  Biochemistry       Date:  1978-08-08       Impact factor: 3.162
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