Literature DB >> 379821

Improved methods for the formation and stabilization of R-loops.

D B Kaback, L M Angerer, N Davidson.   

Abstract

Improved methods for the formation and stabilization of R-loops for visualization in the electron microscope are presented. The two complementary strands of a duplex DNA are photochemically crosslinked once every 1 to 3 kb using 4, 5', 8 trimethylpsoralen. R-loops are then formed by incubation with RNA in 70% formamide at a temperature above the DNA melting temperature. Finally, the R-loops are stabilized by modifying the free single strand of DNA with glyoxal, thus minimizing the displacement of the hybridized RNA by branch migration. In this manner R-loops can be formed and visualized at a high frequency irrespective of the base composition of the nucleic acid of interest.

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Year:  1979        PMID： 379821      PMCID： PMC327868          DOI： 10.1093/nar/6.7.2499

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  29 in total

1.  Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide.

Authors:  J Casey; N Davidson
Journal:  Nucleic Acids Res       Date:  1977       Impact factor: 16.971

2.  Addition of poly(adenylic acid) to RNA using polynucleotide phosphorylase: an improved method for electron microscopic visualization of RNA-DNA hybrids.

Authors:  J D Engel; N Davidson
Journal:  Biochemistry       Date:  1978-09-05       Impact factor: 3.162

3.  Intervening sequence of DNA identified in the structural portion of a mouse beta-globin gene.

Authors:  S M Tilghman; D C Tiemeier; J G Seidman; B M Peterlin; M Sullivan; J V Maizel; P Leder
Journal:  Proc Natl Acad Sci U S A       Date:  1978-02       Impact factor: 11.205

4.  Unique arrangement of coding sequences for 5 S, 5.8 S, 18 S and 25 S ribosomal RNA in Saccharomyces cerevisiae as determined by R-loop and hybridization analysis.

Authors:  P Philippsen; M Thomas; R A Kramer; R W Davis
Journal:  J Mol Biol       Date:  1978-08-15       Impact factor: 5.469

5.  Divergent transcription in the yeast ribosomal RNA coding region as shown by hybridization to separated strands and sequence analysis of cloned DNA.

Authors:  R A Kramer; P Philippsen; R W Davis
Journal:  J Mol Biol       Date:  1978-08-15       Impact factor: 5.469

6.  Detection of long-range sequence order in Drosophila melanogaster satellite DNA IV by a photochemical crosslinking reaction and denaturation microscopy.

Authors:  C J Shen; J E Hearst
Journal:  J Mol Biol       Date:  1977-05-25       Impact factor: 5.469

7.  A novel arrangement of the 18S and 28S sequences in a repeating unit of Drosophila melanogaster rDNA.

Authors:  D M Glover; D S Hogness
Journal:  Cell       Date:  1977-02       Impact factor: 41.582

8.  Immunoglobulin heavy chain gene organization in mice: analysis of a myeloma genomic clone containing variable and alpha constant regions.

Authors:  P W Early; M M Davis; D B Kaback; N Davidson; L Hood
Journal:  Proc Natl Acad Sci U S A       Date:  1979-02       Impact factor: 11.205

9.  Construction and restriction endonuclease mapping of hybrid plasmids containing Saccharomyces cerevisiae ribosomal DNA.

Authors:  J H Cramer; F W Farrelly; J T Barnitz; R H Rownd
Journal:  Mol Gen Genet       Date:  1977-03-16

10.  Analysis of the adult and embryonic chicken globin genes in chromosomal DNA.

Authors:  J D Engel; J B Dodgson
Journal:  J Biol Chem       Date:  1978-11-25       Impact factor: 5.157
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  63 in total

1.  Functional analysis of the yeast genome: use of two-dimensional gel electrophoresis to detect genes in randomly cloned DNA sequences.

Authors:  D Thoraval; M Régnacq; P Neuville; H Boucherie
Journal:  Curr Genet       Date:  1990-11       Impact factor: 3.886

2.  Construction of an ordered clone bank and systematic analysis of the whole transcripts of chromosome VI of Saccharomyces cerevisiae.

Authors:  A Yoshikawa; K Isono
Journal:  Nucleic Acids Res       Date:  1991-03-25       Impact factor: 16.971

3.  Identification and organization of carbon dioxide fixation genes in Xanthobacter flavus H4-14.

Authors:  W G Meijer; A C Arnberg; H G Enequist; P Terpstra; M E Lidstrom; L Dijkhuizen
Journal:  Mol Gen Genet       Date:  1991-02

4.  Identification of a multigene family for small heat shock proteins in soybean and physical characterization of one individual gene coding region.

Authors:  F Schöffl; J L Key
Journal:  Plant Mol Biol       Date:  1983-09       Impact factor: 4.076

5.  Unusual organization and lack of recombination in the ribosomal RNA genes of Coprinus cinereus.

Authors:  J Rhodes Cassidy; D Moore; B C Lu; P J Pukkila
Journal:  Curr Genet       Date:  1984-10       Impact factor: 3.886

6.  Temperature-sensitive lethal mutations on yeast chromosome I appear to define only a small number of genes.

Authors:  D B Kaback; P W Oeller; H Yde Steensma; J Hirschman; D Ruezinsky; K G Coleman; J R Pringle
Journal:  Genetics       Date:  1984-09       Impact factor: 4.562

7.  Meiotic recombination at the ends of chromosomes in Saccharomyces cerevisiae.

Authors:  Arnold B Barton; Michael R Pekosz; Rohini S Kurvathi; David B Kaback
Journal:  Genetics       Date:  2008-06-18       Impact factor: 4.562

8.  Two closely linked transcription units within the 63B heat shock puff locus of D. melanogaster display strikingly different regulation.

Authors:  D O'Connor; J T Lis
Journal:  Nucleic Acids Res       Date:  1981-10-10       Impact factor: 16.971

9.  Isolation and functional analysis of sporulation-induced transcribed sequences from Saccharomyces cerevisiae.

Authors:  E Gottlin-Ninfa; D B Kaback
Journal:  Mol Cell Biol       Date:  1986-06       Impact factor: 4.272

10.  The cloned rRNA genes of P. lophurae: a novel rDNA structure.

Authors:  T R Unnasch; D F Wirth
Journal:  Nucleic Acids Res       Date:  1983-12-10       Impact factor: 16.971
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