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Literature DB >> 743904

Higher order structure in metaphase chromosomes. I. The 250 A fiber.

Abstract

Metaphase chromosomes released from cells in the presence of Joklik's suspension media by vortex-mixing with 0.5 mm glass beads have been analyzed by electron microscopy. In these preparations the chromosomes are composed of series of loops (200-300 A in diameter) which are, in turn, composed of closely-apposed arrays of nucleosomes. Negative-staining of these preparations has allowed the identification of several distinct patterns within the loop which appear to arise from variations in nucleosome packing. Analogous patterns are also observed in chromatin fragments generated by brief micrococcal nuclease digestion. From these data we have deduced certain features of nucleosome-nucleosome interactions in higher-ordered chromatin fibers.

Mesh：

Year: 1978 PMID： 743904 DOI： 10.1007/bf00332139

Source DB: PubMed Journal: Chromosoma ISSN： 0009-5915 Impact factor: 4.316

25 in total

1. Electron microscopy of whole mount metaphase chromosomes.

Authors: J B Rattner; A Branch; B A Hamkalo
Journal: Chromosoma Date: 1975-11-11 Impact factor: 4.316

2. The metaphase chromosome ultrastructure. II. Helical organization of the basic chromosome fiber as revealed by acute angle metal deposition.

Authors: D A Filip; C Gilly; C Mouriquand
Journal: Humangenetik Date: 1975-11-06

Higher order structure in metaphase chromosomes. I. The 250 A fiber.

1. Electron microscopy of whole mount metaphase chromosomes.

2. The metaphase chromosome ultrastructure. II. Helical organization of the basic chromosome fiber as revealed by acute angle metal deposition.

3. Structure of nucleosome core particles of chromatin.

4. Morphological studies of transcription.

Review 5. The structure of mammalian chromosomes.

Review 6. Electronmicroscopy of genetic activity.

7. A super-coil model for nucleohistone.

8. Coiled supercoiled DNA in critical point dried and thin sectioned human chromosome fibres.

9. Visualization of nucleolar genes.

10. Isolation, characterization, and structure of the folded interphase genome of Drosophila melanogaster.

1. Nucleosome binding by the polymerase I transactivator upstream binding factor displaces linker histone H1.

2. Preferential distribution of active RNA polymerase II molecules in the nuclear periphery.

3. Organization within the mammalian kinetochore.

4. Erythroid-specific gene chromatin has an altered association with linker histones.

5. Ultrastructural features of minute chromosomes in a methotrexate-resistant mouse 3T3 cell line.

6. The fine structure of euchromatin and centromeric heterochromatin in Tenebrio molitor chromosomes.

7. Linker DNA accessibility in chromatin fibers of different conformations: a reevaluation.

8. The structure of partly decondensed metaphase chromosomes.

9. Structure of chromatin and the linking number of DNA.

10. Cytological and molecular characterization of centromeres in Mus domesticus and Mus spretus.