Literature DB >> 7388032

The use of rRNA-cellulose chromatography in the rapid isolation of homogeneous protein synthesis initiation factor eIF-2 from rat liver microsomes.

O Nygård, P Westermann, T Hultin.   

Abstract

A rapid and efficient procedure is described for preparing homogeneous polypeptide chain initiation factor eIF-2 from rat liver microsomal salt wash. The method involves ammonium sulfate fractionation, affinity chromatography on rRNA-cellulose and DEAE-cellulose chromatography. The preparation is highly active in initiation complex formation.

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Year:  1980        PMID： 7388032     DOI： 10.1016/0005-2787(80)90148-3

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  5 in total

1.  Interaction of a cellular 57-kilodalton protein with the internal translation initiation site of foot-and-mouth disease virus.

Authors:  N Luz; E Beck
Journal:  J Virol       Date:  1991-12       Impact factor: 5.103

2.  Effect of vasopressin on the regulation of protein synthesis initiation in liver cells.

Authors:  J Menaya; R Parrilla; M S Ayuso
Journal:  Biochem J       Date:  1988-09-15       Impact factor: 3.857

3.  Specific interaction of one subunit of eukaryotic initiation factor eIF-3 with 18S ribosomal RNA within the binary complex, eIF-3 small ribosomal subunit, as shown by cross-linking experiments.

Authors:  O Nygård; P Westermann
Journal:  Nucleic Acids Res       Date:  1982-02-25       Impact factor: 16.971

4.  Cross-linking of Met-tRNAf to eIF-2 beta and to the ribosomal proteins S3a and S6 within the eukaryotic inhibition complex, eIF-2 .GMPPCP.Met-tRNAf.small ribosomal subunit.

Authors:  P Westermann; O Nygård; H Bielka
Journal:  Nucleic Acids Res       Date:  1981-05-25       Impact factor: 16.971

5.  A mechanism by which adenovirus virus-associated RNAI controls translation in a transient expression assay.

Authors:  G Akusjärvi; C Svensson; O Nygård
Journal:  Mol Cell Biol       Date:  1987-01       Impact factor: 4.272
  5 in total

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