Literature DB >> 6910637

Cross-linking of Met-tRNAf to eIF-2 beta and to the ribosomal proteins S3a and S6 within the eukaryotic inhibition complex, eIF-2 .GMPPCP.Met-tRNAf.small ribosomal subunit.

P Westermann, O Nygård, H Bielka.   

Abstract

In the quaternary initiation complex, eIF-2.GMPPCP.Met-tRNAf.40S ribosomal subunit, the Met-tRNAf can be cross-linked to the beta subunit of initiation factor eIF-2 as well as to ribosomal proteins S3a and S6 by treatment with the bifunctional reagent, diepoxybutane. Using 40S subunits, modified in advance with the heterobifunctional reagent, methyl-rho-azido-benzoylaminoacetimidate, Met-tRNAf is covalently bound to the same ribosomal proteins (S3a and S6) upon irradiation of the complex with ultraviolet light. Under both conditions proteins S3a and S6, together with a limited number of other ribosomal proteins, are covalently bound to 18S ribosomal RNA.

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Year:  1981        PMID： 6910637      PMCID： PMC326852          DOI： 10.1093/nar/9.10.2387

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  27 in total

1.  Binding of MET-TRNAf and GTP to homogeneous initiation factor MP.

Authors:  B Safer; S L Adams; W F Anderson; W C Merrick
Journal:  J Biol Chem       Date:  1975-12-10       Impact factor: 5.157

2.  Phosphorylation of double-stranded DNAs by T4 polynucleotide kinase.

Authors:  J R Lillehaug; R K Kleppe; K Kleppe
Journal:  Biochemistry       Date:  1976-05-04       Impact factor: 3.162

3.  Purification and physical properties of homogeneous initiation factor MP from rabbit reticulocytes.

Authors:  B Safer; W F Anderson; W C Merrick
Journal:  J Biol Chem       Date:  1975-12-10       Impact factor: 5.157

4.  Initiation of eukaryotic protein synthesis: (Met-tRNA f -40S ribosome) initiation complex catalysed by purified initiation factors in the absence of mRNA.

Authors:  M H Schreier; T Staehelin
Journal:  Nat New Biol       Date:  1973-03-14

5.  Studies on proteins of animal ribosomes. 13. Enumeration of ribosomal proteins of rat liver.

Authors:  H Welfle; J Stahl; H Bielka
Journal:  FEBS Lett       Date:  1972-10-01       Impact factor: 4.124

6.  Met-tRNA hydrolase from reticulocytes specific for Met-tRNA f Met on 40S ribosomal subunits.

Authors:  J Morrisey; B Hardesty
Journal:  Arch Biochem Biophys       Date:  1972-09       Impact factor: 4.013

7.  Phosphorylation of nucleic acid by an enzyme from T4 bacteriophage-infected Escherichia coli.

Authors:  C C Richardson
Journal:  Proc Natl Acad Sci U S A       Date:  1965-07       Impact factor: 11.205

8.  Dissociation of mammalian polyribosomes into subunits by puromycin.

Authors:  G Blobel; D Sabatini
Journal:  Proc Natl Acad Sci U S A       Date:  1971-02       Impact factor: 11.205

9.  Protein initiation in eukaryotes: formation and function of a ternary complex composed of a partially purified ribosomal factor, methionyl transfer RNA, and guanosine triphosphate.

Authors:  D H Levin; D Kyner; G Acs
Journal:  Proc Natl Acad Sci U S A       Date:  1973-01       Impact factor: 11.205

10.  The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent.

Authors:  A E Bolton; W M Hunter
Journal:  Biochem J       Date:  1973-07       Impact factor: 3.857
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  14 in total

Review 1.  Mechanism and regulation of eukaryotic protein synthesis.

Authors:  W C Merrick
Journal:  Microbiol Rev       Date:  1992-06

2.  Genetic and biochemical interactions among Yar1, Ltv1 and Rps3 define novel links between environmental stress and ribosome biogenesis in Saccharomyces cerevisiae.

Authors:  Jesse W Loar; Robert M Seiser; Alexandra E Sundberg; Holly J Sagerson; Nasreen Ilias; Pamela Zobel-Thropp; Elizabeth A Craig; Deborah E Lycan
Journal:  Genetics       Date:  2004-12       Impact factor: 4.562

3.  Probing the structure of mouse Ehrlich ascites cell 5.8S, 18S and 28S ribosomal RNA in situ.

Authors:  L Holmberg; Y Melander; O Nygård
Journal:  Nucleic Acids Res       Date:  1994-04-25       Impact factor: 16.971

4.  Nuclear and nucleolar targeting of human ribosomal protein S6.

Authors:  C Schmidt; E Lipsius; J Kruppa
Journal:  Mol Biol Cell       Date:  1995-12       Impact factor: 4.138

5.  Cross-linking of mRNA to initiation factor eIF-3, 24 kDa cap binding protein and ribosomal proteins S1, S3/3a, S6 and S11 within the 48S pre-initiation complex.

Authors:  P Westermann; O Nygård
Journal:  Nucleic Acids Res       Date:  1984-12-11       Impact factor: 16.971

6.  Probing the conformational changes in 5.8S, 18S and 28S rRNA upon association of derived subunits into complete 80S ribosomes.

Authors:  L Holmberg; Y Melander; O Nygård
Journal:  Nucleic Acids Res       Date:  1994-07-25       Impact factor: 16.971

7.  Influence of the state of ribosome association on the phosphorylation of ribosomal proteins in isolated ribosome--protein kinase systems from rat cerebral cortex.

Authors:  T A Francis; S Roberts
Journal:  Biochem J       Date:  1982-11-15       Impact factor: 3.857

8.  Components of the multifactor complex needed for internal initiation by the IRES of hepatitis C virus in Saccharomyces cerevisiae.

Authors:  Amy B Rosenfeld; Vincent R Racaniello
Journal:  RNA Biol       Date:  2010-09-01       Impact factor: 4.652

9.  Isolation, sequencing, and characterization of crp-5, a gene encoding a Neurospora ribosomal protein.

Authors:  Z Wang; K A Tarawneh; S J Free
Journal:  Curr Genet       Date:  1993       Impact factor: 3.886

10.  Identification of ribosomal protein S3a as a candidate for a novel PI 3-kinase target in the nucleus.

Authors:  Norihisa Hamaguchi; Tsutomu Ohdaira; Azusa Shinohara; Akihiro Iwamatsu; Sayoko Ihara; Yasuhisa Fukui
Journal:  Cytotechnology       Date:  2002-11       Impact factor: 2.058
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