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Literature DB >> 7364713

High-frequency heterokaryon formation by Mucor racemosus.

Abstract

Heterokaryons from Mucor racemosus were produced from two auxotrophic strains of the fungus. Germlings were converted to spheroplasts by using commercial chitinase and purified Myxobacter AL-1 chitosanase. Spheroplasts from the auxotrophic strains were mixed and fused in solutions of polyethylene glycol and CaCl2. Under optimal conditions, prototrophic heterokaryons were formed at a frequency of about 5%.

Entities: Chemical Species

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Year: 1980 PMID： 7364713 PMCID： PMC293660 DOI： 10.1128/jb.141.2.565-569.1980

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

11 in total

1. Conditions for induced fusion of fungal protoplasts in polyethylene glycol solutions.

Authors: J Anné; J F Peberdy
Journal: Arch Microbiol Date: 1975-11-07 Impact factor: 2.552

2. Protoplast fusion of Schizosaccharomyces pombe Auxotrophic mutants of identical mating-type.

Authors: M Sipiczki; L Ferenczy
Journal: Mol Gen Genet Date: 1977-02-28

3. Interspecific protoplast fusion and complementation in Aspergilli.

Authors: L Ferenczy; M Szegedi; F Kevei
Journal: Experientia Date: 1977-02-15

4. Respiratory-deficient, yeastlike mutant of Mucor.

Authors: R Storck; R C Morrill
Journal: Biochem Genet Date: 1971-10 Impact factor: 1.890

5. Fusion of bacterial protoplasts.

Authors: P Schaeffer; B Cami; R D Hotchkiss
Journal: Proc Natl Acad Sci U S A Date: 1976-06 Impact factor: 11.205

6. Spheroplast fusion and heterokaryon formation in Mucor racemosus.

Authors: F J Genthner; P T Borgia
Journal: J Bacteriol Date: 1978-04 Impact factor: 3.490

7. Formation and reversion of Streptomycete protoplasts: cultural condition and morphological study.

Authors: M Okanishi; K Suzuki; H Umezawa
Journal: J Gen Microbiol Date: 1974-02

8. Control of beta-glucosidase synthesis in Mucor racemosus.

Authors: P Borgia; P S Sypherd
Journal: J Bacteriol Date: 1977-05 Impact factor: 3.490

9. Transformation of yeast.

Authors: A Hinnen; J B Hicks; G R Fink
Journal: Proc Natl Acad Sci U S A Date: 1978-04 Impact factor: 11.205

10. Extracellular enzyme from Myxobacter AL-1 that exhibits both beta-1,4-glucanase and chitosanase activites.

Authors: A Hedges; R S Wolfe
Journal: J Bacteriol Date: 1974-11 Impact factor: 3.490

14 in total

1. Integrative transformation by homologous recombination in the zygomycete Mucor circinelloides.

Authors: J Arnau; L P Jepsen; P Strøman
Journal: Mol Gen Genet Date: 1991-02

Review 2. Mucor dimorphism.

Authors: M Orlowski
Journal: Microbiol Rev Date: 1991-06

3. Gene replacement and ectopic integration in the zygomycete Mucor circinelloides.

Authors: J Arnau; P Strøman
Journal: Curr Genet Date: 1993 May-Jun Impact factor: 3.886

4. Genetic evidence for independence between fermentative metabolism (ethanol accumulation) and yeast-cell development in the dimorphic fungus Mucor rouxii.

Authors: J C Torres-Guzman; G A Arreola-Garcia; R Zazueta-Sandoval; T Carrillo-Rayas; G Martinez-Cadena; F Gutierrez-Corona
Journal: Curr Genet Date: 1994-08 Impact factor: 3.886