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Literature DB >> 242297

Conditions for induced fusion of fungal protoplasts in polyethylene glycol solutions.

Abstract

Solutions containing polyethylene glycol MW 6000 (PEG) induced fusion of protoplasts of Penicillium chrysogenum. Balanced heterokaryons were formed by fusion of nutritionally complementing protoplasts. Heterokaryotic fusion products were obtained up to a frequency of 4% of the number of protoplasts, surviving the fusion treatment. Investigation of the conditions, necessary to achieve this high fusion frequency, showed that supplementing the PEG solution with Ca++ and adjustment to high pH gave the best results. Mechanisms of fusion of fungal protoplasts by PEG, calcium and alkaline pH are discussed in view of the obtained results.

Entities: Chemical Disease Species

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Year: 1975 PMID： 242297 DOI： 10.1007/bf00447138

Source DB: PubMed Journal: Arch Microbiol ISSN： 0302-8933 Impact factor: 2.552

11 in total

1. ISOLATION OF AUXOTROPHS OF PENICILLIUM CHRYSOGENUM AND THEIR PENICILLIN YIELDS.

Authors: K D MACDONALD; J M HUTCHINSON; W A GILLETT
Journal: J Gen Microbiol Date: 1963-12

2. Mechanisms of cell fusion.

Authors: Q F Ahkong; D Fisher; W Tampion; J A Lucy
Journal: Nature Date: 1975-01-17 Impact factor: 49.962

3. Para-sexual recombination in Penicillium chrysogenum.

Authors: G PONTECORVO; G SERMONTI
Journal: J Gen Microbiol Date: 1954-08

4. A method for high-frequency intergeneric fusion of plant protoplasts.

Authors: K N Kao; M R Michayluk
Journal: Planta Date: 1974-12 Impact factor: 4.116

5. The isolation, regeneration and fusion of Phycomyces protoplasts.

Authors: H Binding
Journal: Mol Gen Genet Date: 1974

6. The effect of high pH and calcium on tobacco leaf protoplast fusion.

Authors: W A Keller; G Melchers
Journal: Z Naturforsch C Date: 1973 Nov-Dec Impact factor: 1.649

7. Chemically-induced and thermally-induced cell fusion: lipid-lipid interactions.

Authors: Q F Ahkong; F C Cramp; D Fisher; J I Howell; W Tampion; M Verrinder; J A Lucy
Journal: Nat New Biol Date: 1973-04-18

8. Membrane fusion and molecular segregation in phospholipid vesicles.

Authors: D Papahadjopoulos; G Poste; B E Schaeffer; W J Vail
Journal: Biochim Biophys Acta Date: 1974-05-30

9. Formation and regeneration of Penicillium chrysogenum protoplasts.

Authors: J Anné; H Eyssen; P De Somer
Journal: Arch Microbiol Date: 1974-07-04 Impact factor: 2.552

10. Ca 2+ -induced fusion of avian erythrocytes.

Authors: Z Toister; A Loyter
Journal: Biochim Biophys Acta Date: 1971-08-13

15 in total

1. Parameters governing bacterial regeneration and genetic recombination after fusion of Bacillus subtilis protoplasts.

Authors: M H Gabor; R D Hotchkiss
Journal: J Bacteriol Date: 1979-03 Impact factor: 3.490

2. Genetics of speciation in the basidiomycetous genus Polyporus.

Authors: P Hoffmann; K Esser
Journal: Theor Appl Genet Date: 1978-11 Impact factor: 5.699

3. Construction of lactose-assimilating and high-ethanol-producing yeasts by protoplast fusion.

Authors: F Farahnak; T Seki; D D Ryu; D Ogrydziak
Journal: Appl Environ Microbiol Date: 1986-02 Impact factor: 4.792

4. Intraspecific hybridization between Coprinus cinereus and Schizophyllum commune by PEG-induced protoplast fusion and electrofusion.

Authors: J Zhao; S T Chang
Journal: World J Microbiol Biotechnol Date: 1995-09 Impact factor: 3.312