Literature DB >> 7358131

A logical approach to the isolation of lactate dehydrogenase isozyme X from human testes: a general rationale for the isolation of homotetrameric LDH isozymes.

P Toowicharanont, J Svasti.   

Abstract

Immunoadsorbent and oxamate-Sepharose chromatography were used to isolate electrophoretically homogeneous LDH-X from human testes with a final specific activity of 125 IU/mg and good yields: other applications of this appraoch are discussed.

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Year:  1980        PMID： 7358131     DOI： 10.1007/bf02003956

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  12 in total

1.  Affinity chromatography of lactate dehydrogenase Model studies demonstrating the potential of the technique in the mechanistic investigation as well as in the purification of multi-substrate enzymes.

Authors:  P O'Carra; S Barry
Journal:  FEBS Lett       Date:  1972-04-01       Impact factor: 4.124

2.  AN UNUSUAL ISOZYME OF LACTATE DEHYDROGENASE IN MATURE TESTES: LOCALIZATION, ONTOGENY, AND KINETIC PROPERTIES.

Authors:  W H ZINKHAM; A BLANCO; L J CLOWRY
Journal:  Ann N Y Acad Sci       Date:  1964-12-28       Impact factor: 5.691

3.  Pregnancy suppression by an antiserum to the sperm specific lactate dehydrogenase.

Authors:  E Goldberg; J Lerum
Journal:  Science       Date:  1972-05-12       Impact factor: 47.728

4.  Isolation of human lactate dehydrogenase isoenzyme X by affinity chromatography.

Authors:  A H Kolk; L van Kuyk; B Boettcher
Journal:  Biochem J       Date:  1978-09-01       Impact factor: 3.857

5.  Immunological impairment of pregnancy in mice by lactate dehydrogenase-X.

Authors:  J E Lerum; E Goldberg
Journal:  Biol Reprod       Date:  1974-08       Impact factor: 4.285

6.  Molecular basis for multiple forms of LDH-X.

Authors:  E Goldberg
Journal:  J Exp Zool       Date:  1973-12

7.  [Cross-reacting antigenic determinants on lactate dehydrogenases I and V by acetylation].

Authors:  K Rajewsky
Journal:  Biochim Biophys Acta       Date:  1966-05-26

8.  A general method for the specific isolation of peptides containing modified residues, using insoluble antibody columns.

Authors:  M Wilchek; V Bocchini; M Becker; D Givol
Journal:  Biochemistry       Date:  1971-07-20       Impact factor: 3.162

9.  Immunochemical specificity of lactate dehydrogenase-X.

Authors:  E Goldberg
Journal:  Proc Natl Acad Sci U S A       Date:  1971-02       Impact factor: 11.205

10.  Lactate Dehydrogenases in Human Testes.

Authors:  A Blanco; W H Zinkham
Journal:  Science       Date:  1963-02-15       Impact factor: 47.728
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  1 in total

1.  Separation of rat lactate dehydrogenase isoenzyme C4 from other isoenzymes by affinity and ion-exchange chromatography.

Authors:  A A Ansari
Journal:  Biochem J       Date:  1981-10-01       Impact factor: 3.857
  1 in total

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