Literature DB >> 213050

Isolation of human lactate dehydrogenase isoenzyme X by affinity chromatography.

A H Kolk, L van Kuyk, B Boettcher.   

Abstract

Human isoenzyme LDH-X (lactate dehydrogenase isoenzyme X) was isolated from seminal fluid of frozen semen samples by affinity chromatography by using oxamate-Sepharose and AMP-Sepharose. In the presence of 1.6 mM-NAD+, isoenzyme LDH-X does not bind to AMP-Sepharose, whereas the other lactate dehydrogenase isoenzymes do. This is the crucial point in the isolation of isoenzyme LDH-X from the other isoenzymes. The purified human isoenzyme LDH-X had a specific activity of 146 units/mg of protein.

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Year:  1978        PMID： 213050      PMCID： PMC1185842          DOI： 10.1042/bj1730767

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  19 in total

1.  Affinity chromatography of lactate dehydrogenase Model studies demonstrating the potential of the technique in the mechanistic investigation as well as in the purification of multi-substrate enzymes.

Authors:  P O'Carra; S Barry
Journal:  FEBS Lett       Date:  1972-04-01       Impact factor: 4.124

2.  Lactate dehydrogenase-X from mouse testes and spermatozoa.

Authors:  E Goldberg
Journal:  Methods Enzymol       Date:  1975       Impact factor: 1.600

3.  Pregnancy suppression by an antiserum to the sperm specific lactate dehydrogenase.

Authors:  E Goldberg; J Lerum
Journal:  Science       Date:  1972-05-12       Impact factor: 47.728

4.  Affinity chromatographic differentiation of lactate dehydrogenase isoenzymes on the basis of differential abortive complex formation.

Authors:  P O'Carra; S Barry; E Corcoran
Journal:  FEBS Lett       Date:  1974-07-15       Impact factor: 4.124

5.  Separation and quantitation of lactic dehydrogenase isoenzymes by disc electrophoresis.

Authors:  A A Dietz; T Lubrano
Journal:  Anal Biochem       Date:  1967-08       Impact factor: 3.365

6.  Immunological impairment of pregnancy in mice by lactate dehydrogenase-X.

Authors:  J E Lerum; E Goldberg
Journal:  Biol Reprod       Date:  1974-08       Impact factor: 4.285

7.  The separation of lactate dehydrogenase X from other lactate dehydrogenase isozymes of mouse testes by affinity chromatography.

Authors:  H Spielmann; R P Erickson; C J Epstein
Journal:  FEBS Lett       Date:  1973-09-01       Impact factor: 4.124

8.  Immunochemical specificity of lactate dehydrogenase-X.

Authors:  E Goldberg
Journal:  Proc Natl Acad Sci U S A       Date:  1971-02       Impact factor: 11.205

9.  Organ specificity and lactate-dehydrogenase activity. Some properties of human spermatozoal lactate dehydrogenase.

Authors:  J H Wilkinson; W A Withycombe
Journal:  Biochem J       Date:  1965-12       Impact factor: 3.857

10.  Lactate Dehydrogenases in Human Testes.

Authors:  A Blanco; W H Zinkham
Journal:  Science       Date:  1963-02-15       Impact factor: 47.728
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  3 in total

1.  Separation of rat lactate dehydrogenase isoenzyme C4 from other isoenzymes by affinity and ion-exchange chromatography.

Authors:  A A Ansari
Journal:  Biochem J       Date:  1981-10-01       Impact factor: 3.857

2.  A logical approach to the isolation of lactate dehydrogenase isozyme X from human testes: a general rationale for the isolation of homotetrameric LDH isozymes.

Authors:  P Toowicharanont; J Svasti
Journal:  Experientia       Date:  1980-01-15

3.  Properties of human testis-specific lactate dehydrogenase expressed from Escherichia coli.

Authors:  K M LeVan; E Goldberg
Journal:  Biochem J       Date:  1991-02-01       Impact factor: 3.857
  3 in total

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