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Literature DB >> 729750

A rapid and simple method for the isolation of pure eosinophilic leukocytes from horse blood.

A Jörg, P Portmann, G Fellay, J L Dreyer, J Meyer.

Abstract

An improved and short method is described for the isolation of intact eosinophilic leukocytes from horse blood with high yield (1--1.5 g/20 l). Viability and purity of the preparations were verified by light and electron microscopy and by the trypan blue exclusion test. Isolated eosinophils were 98--100% pure, intact and viable, and they could be shown to phagocytise immune-complexes.

Entities: Chemical Species

Mesh：

Year: 1978 PMID： 729750 DOI： 10.1007/bf02034734

Source DB: PubMed Journal: Experientia ISSN： 0014-4754

4 in total

1. Studies with eosinophil leucocytes isolated from the blood of the horse.

Authors: R K ARCHER
Journal: Br J Haematol Date: 1960-07 Impact factor: 6.998

2. Partial characterization of the protein components of eosinophil granules isolated from guinea pig exudates.

Authors: T P Gessner; R Himmelhoch; E Shelton
Journal: Arch Biochem Biophys Date: 1973-06 Impact factor: 4.013

3. Isolation and partial characterization of human eosinophil granules. Comparison to neutrophils.

Authors: B C West; N A Gelb; A S Rosenthal
Journal: Am J Pathol Date: 1975-12 Impact factor: 4.307

4. Arginine-rich cationic proteins of human eosinophil granules: comparison of the constituents of eosinophilic and neutrophilic leukocytes.

Authors: I Olsson; P Venge; J K Spitznagel; R I Lehrer
Journal: Lab Invest Date: 1977-05 Impact factor: 5.662

4 in total

8 in total

1. Identification and isolation of subpopulations of pleural cells by multiparameter flow cytometry.

Authors: B E Lehnert; J A Steinkamp
Journal: Cell Biophys Date: 1986-06

2. Cells and inflammation: modern trends and technical outlook.

Authors:
Journal: Klin Wochenschr Date: 1984-05-15

3. An improved method for the isolation of eosinophilic leukocytes from normal human blood.

Authors: P Portmann; P Köppel; A Jörg
Journal: Experientia Date: 1980-01-15

4. Mechanism of nitrite oxidation by eosinophil peroxidase: implications for oxidant production and nitration by eosinophils.

Authors: Christine J van Dalen; Christine C Winterbourn; Anthony J Kettle
Journal: Biochem J Date: 2006-03-15 Impact factor: 3.857

A rapid and simple method for the isolation of pure eosinophilic leukocytes from horse blood.

1. Studies with eosinophil leucocytes isolated from the blood of the horse.

2. Partial characterization of the protein components of eosinophil granules isolated from guinea pig exudates.

3. Isolation and partial characterization of human eosinophil granules. Comparison to neutrophils.

4. Arginine-rich cationic proteins of human eosinophil granules: comparison of the constituents of eosinophilic and neutrophilic leukocytes.

1. Identification and isolation of subpopulations of pleural cells by multiparameter flow cytometry.

2. Cells and inflammation: modern trends and technical outlook.

3. An improved method for the isolation of eosinophilic leukocytes from normal human blood.

4. Mechanism of nitrite oxidation by eosinophil peroxidase: implications for oxidant production and nitration by eosinophils.

5. Substrates and products of eosinophil peroxidase.

6. Isolation of bovine eosinophils and characterization of their leukotriene formation.

7. Horse eosinophil degranulation induced by the ionophore A23187. Ultrastructure and role of phospholipase A2.

8. Leukotriene generation by eosinophils.