Literature DB >> 7305939

Studies of pyruvate-water isotope exchange catalysed by erythrocytes and proteins.

R J Simpson, K M Brindle, F F Brown, I D Campbell, D L Foxall.   

Abstract

Erythrocyte suspensions in buffer made with 2H2O catalyse the exchange of pyruvate protons. This process can be easly observed by spin-echo proton magnetic resonance. The dominant exchange process is shown to be due to the formation of Schiff-base links between pyruvate and amino groups of haemoglobin. Other proteins with free alpha-amino groups also catalyse the exchange. The pH*-dependence of the exchange rate due to hen-egg-white-lysozyme reflects the dissociation of the alpha-amino group.

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Year:  1981        PMID： 7305939      PMCID： PMC1162619          DOI： 10.1042/bj1930401

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  18 in total

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Authors:  T SHINODA
Journal:  Biochim Biophys Acta       Date:  1965-02-15

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Authors:  J Katz; R Rognstad
Journal:  Curr Top Cell Regul       Date:  1976

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Authors:  M H Garner; R A Bogardt; F R Gurd
Journal:  J Biol Chem       Date:  1975-06-25       Impact factor: 5.157

4.  The metabolism of l-[3-3h]lactate by isolated hamster liver cells.

Authors:  R Rognstad; P Wals
Journal:  Biochim Biophys Acta       Date:  1976-06-23

5.  Transport of pyruvate nad lactate into human erythrocytes. Evidence for the involvement of the chloride carrier and a chloride-independent carrier.

Authors:  A P Halestrap
Journal:  Biochem J       Date:  1976-05-15       Impact factor: 3.857

6.  X-ray diffraction study of binding of 2,3-diphosphoglycerate to human deoxyhaemoglobin.

Authors:  A Arnone
Journal:  Nature       Date:  1972-05-19       Impact factor: 49.962

7.  The reaction of 2,4,6-trinitrobenzenesulphonic acid with amino acids, Peptides and proteins.

Authors:  R B Freedman; G K Radda
Journal:  Biochem J       Date:  1968-07       Impact factor: 3.857

8.  Stereoselective interactions of phosphoenolpyruvate analogues with phosphoenolpyruvate-utilizing enzymes.

Authors:  T Nowak; A S Mildvan
Journal:  J Biol Chem       Date:  1970-11-25       Impact factor: 5.157

9.  Magnitude of the equilibrium isotope effect on carbon-tritium bond synthesis.

Authors:  H P Meloche; C T Monti; W W Cleland
Journal:  Biochim Biophys Acta       Date:  1977-02-09

10.  Potassium cyanate as an inhibitor of the sickling of erythrocytes in vitro.

Authors:  A Cerami; J M Manning
Journal:  Proc Natl Acad Sci U S A       Date:  1971-06       Impact factor: 11.205
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  4 in total

1.  13C and 1H nuclear magnetic resonance study of glycogen futile cycling in strains of the genus Fibrobacter.

Authors:  C Matheron; A M Delort; G Gaudet; E Forano; T Liptaj
Journal:  Appl Environ Microbiol       Date:  1998-01       Impact factor: 4.792

2.  A p.m.r. isotope-exchange method for studying the kinetic properties of dehydrogenases in intact cells.

Authors:  R J Simpson; K M Brindle; F F Brown; I D Campbell; D L Foxall
Journal:  Biochem J       Date:  1982-03-15       Impact factor: 3.857

3.  Effects of deuteration on transamination and oxidation of hyperpolarized 13C-Pyruvate in the isolated heart.

Authors:  Alexander M Funk; Xiaodong Wen; Thomas Hever; Nesmine R Maptue; Chalermchai Khemtong; A D Sherry; Craig R Malloy
Journal:  J Magn Reson       Date:  2019-03-04       Impact factor: 2.229

4.  A 1H n.m.r. study of isotope exchange catalysed by glycolytic enzymes in the human erythrocyte.

Authors:  K M Brindle; F F Brown; I D Campbell; D L Foxall; R J Simpson
Journal:  Biochem J       Date:  1982-03-15       Impact factor: 3.857
  4 in total

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