Literature DB >> 7092831

A p.m.r. isotope-exchange method for studying the kinetic properties of dehydrogenases in intact cells.

R J Simpson, K M Brindle, F F Brown, I D Campbell, D L Foxall.   

Abstract

A method to determine the activity of dehydrogenases in an intact-cell system is described. The method involves the use of n.m.r. to monitor bulk isotope exchange. The approach is illustrated by application to the isotope equilibration of pyruvate and lactate as catalyzed by lactate dehydrogenase in intact erythrocytes. Particular problems peculiar to bulk isotope exchange and its observation by n.m.r. are considered.

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Year:  1982        PMID： 7092831      PMCID： PMC1158150          DOI： 10.1042/bj2020573

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  19 in total

1.  The nature of the substrate inhibition in lactate dehydrogenases as studied by a spin-labeled derivative of NAD.

Authors:  W E Trommer; H Huth; H R Wenzel
Journal:  Biochim Biophys Acta       Date:  1979-03-16

2.  Human erythrocyte metabolism studies by 1H spin echo NMR.

Authors:  F F Brown; I D Campbell; P W Kuchel; D C Rabenstein
Journal:  FEBS Lett       Date:  1977-10-01       Impact factor: 4.124

3.  Stereoselective, SH-dependent transfer of lactate in mammalian erythrocytes.

Authors:  B Deuticke; I Rickert; E Beyer
Journal:  Biochim Biophys Acta       Date:  1978-02-02

4.  Transport of pyruvate nad lactate into human erythrocytes. Evidence for the involvement of the chloride carrier and a chloride-independent carrier.

Authors:  A P Halestrap
Journal:  Biochem J       Date:  1976-05-15       Impact factor: 3.857

Review 5.  The application of high resolution nuclear magnetic resonance to biological systems.

Authors:  I D Campbell; C M Dobson
Journal:  Methods Biochem Anal       Date:  1979

6.  Role of ions in the regulation of porcine lactate dehydrogenase.

Authors:  E Rivedal; T Sanner
Journal:  Biochim Biophys Acta       Date:  1979-03-16

7.  Molecular kinetics of beef heart lactate dehydrogenase.

Authors:  U Borgmann; T W Moon; K J Laidler
Journal:  Biochemistry       Date:  1974-12-03       Impact factor: 3.162

Review 8.  Lactate dehydrogenases: structure and function.

Authors:  J Everse; N O Kaplan
Journal:  Adv Enzymol Relat Areas Mol Biol       Date:  1973

9.  The redox state of free nicotinamide-adenine dinucleotide in the cytoplasm and mitochondria of rat liver.

Authors:  D H Williamson; P Lund; H A Krebs
Journal:  Biochem J       Date:  1967-05       Impact factor: 3.857

10.  Measured and calculated NAD+-NADH ratios in human erythrocytes.

Authors:  W E Marshall; A Omachi
Journal:  Biochim Biophys Acta       Date:  1974-06-20
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  8 in total

1.  The effect of exogenous substrate concentrations on true and apparent metabolism of hyperpolarized pyruvate in the isolated perfused lung.

Authors:  Stephen Kadlecek; Hoora Shaghaghi; Sarmad Siddiqui; Harrilla Profka; Mehrdad Pourfathi; Rahim Rizi
Journal:  NMR Biomed       Date:  2014-10-20       Impact factor: 4.044

2.  A proton n.m.r. study of iminodipeptide transport and hydrolysis in the human erythrocyte. Possible physiological roles for the coupled system.

Authors:  G F King; P W Kuchel
Journal:  Biochem J       Date:  1984-06-01       Impact factor: 3.857

3.  Studies of lactate dehydrogenase in the purified state and in intact erythrocytes.

Authors:  R J Simpson; K M Brindle; F F Brown; I D Campbell; D L Foxall
Journal:  Biochem J       Date:  1982-03-15       Impact factor: 3.857

4.  Cerebral dynamics and metabolism of hyperpolarized [1-(13)C]pyruvate using time-resolved MR spectroscopic imaging.

Authors:  Ralph E Hurd; Yi-Fen Yen; James Tropp; Adolf Pfefferbaum; Daniel M Spielman; Dirk Mayer
Journal:  J Cereb Blood Flow Metab       Date:  2010-06-30       Impact factor: 6.200

5.  Effects of deuteration on transamination and oxidation of hyperpolarized 13C-Pyruvate in the isolated heart.

Authors:  Alexander M Funk; Xiaodong Wen; Thomas Hever; Nesmine R Maptue; Chalermchai Khemtong; A D Sherry; Craig R Malloy
Journal:  J Magn Reson       Date:  2019-03-04       Impact factor: 2.229

6.  Hyperpolarized [1-13C]pyruvate-to-[1-13C]lactate conversion is rate-limited by monocarboxylate transporter-1 in the plasma membrane.

Authors:  Yi Rao; Seth Gammon; Niki M Zacharias; Tracy Liu; Travis Salzillo; Yuanxin Xi; Jing Wang; Pratip Bhattacharya; David Piwnica-Worms
Journal:  Proc Natl Acad Sci U S A       Date:  2020-08-24       Impact factor: 11.205

7.  A 1H n.m.r. study of isotope exchange catalysed by glycolytic enzymes in the human erythrocyte.

Authors:  K M Brindle; F F Brown; I D Campbell; D L Foxall; R J Simpson
Journal:  Biochem J       Date:  1982-03-15       Impact factor: 3.857

8.  (13) C magnetic resonance spectroscopy measurements with hyperpolarized [1-(13) C] pyruvate can be used to detect the expression of transgenic pyruvate decarboxylase activity in vivo.

Authors:  Piotr Dzien; Sui-Seng Tee; Mikko I Kettunen; Scott K Lyons; Timothy J Larkin; Kerstin N Timm; De-En Hu; Alan Wright; Tiago B Rodrigues; Eva M Serrao; Irene Marco-Rius; Elizabeth Mannion; Paula D'Santos; Brett W C Kennedy; Kevin M Brindle
Journal:  Magn Reson Med       Date:  2015-09-21       Impact factor: 4.668
  8 in total

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