Literature DB >> 7305936

The penicillin-binding site in the exocellular DD-carboxypeptidase-transpeptidase of Actinomadura R39.

C Duez, B Joris, J M Frère, J M Ghuysen, J Van Beeumen.   

Abstract

Heat denaturation and Pronase degradation of the complex previously formed between benzylpenicillin and the exocellular DD-carboxypeptidase-transpeptidase of Actinomadura R39 yields a heptapeptide H-Leu-Pro-Ala-Ser-Asn-Gly-Val-OH, where the benzylpenicilloyl group is ester-linked to the serine residue. This linkage is very labile and its hydrolysis causes the release of benzylpenicilloate. In contrast, the native benzylpenicilloyl-enzyme complex is very stable (half-life 70 h at 37 degrees C) and its breakdown proceeds via fragmentation of the bound benzylpenicilloyl group [Fuad, Frère, Ghuysen, Duez & Iwatsubo (1976) Biochem. J. 155, 623-629].

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Year:  1981        PMID： 7305936      PMCID： PMC1162578          DOI： 10.1042/bj1930083

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  14 in total

1.  Fragmentation of benzylpenicillin after interaction with the exocellular DD-carboxypeptidase-transpeptidases of Streptomyces R61 and R39.

Authors:  J Frere; J Ghuysen; J Degelaen; A Loffet; H R Perkins
Journal:  Nature       Date:  1975-11-13       Impact factor: 49.962

2.  Occurrence of a serine residue in the penicillin-binding site of the exocellular DD-carboxy-peptidase-transpeptidase from Streptomyces R61.

Authors:  J M Frère; C Duez; J M Ghuysen; J Vandekerkhove
Journal:  FEBS Lett       Date:  1976-11       Impact factor: 4.124

3.  Amino-terminal sequence analysis of proteins purified on a nanomole scale by gel electrophoresis.

Authors:  A M Weiner; T Platt; K Weber
Journal:  J Biol Chem       Date:  1972-05-25       Impact factor: 5.157

4.  Sequence analysis of fluorescamine-stained peptides and proteins purified on a nanomole scale. Application to proteins of bacteriophage MS2.

Authors:  J Vandekerckhove; M Van Montagu
Journal:  Eur J Biochem       Date:  1974-05-02

5.  Chemical studies on methionyl-tRNA synthetase from Escherichia coli.

Authors:  C J Bruton; B S Hartley
Journal:  J Mol Biol       Date:  1970-09-14       Impact factor: 5.469

6.  Beta-lactamase inactivation by mechanism-based reagents.

Authors:  J Fisher; J G Belasco; R L Charnas; S Khosla; J R Knowles
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  1980-05-16       Impact factor: 6.237

7.  Fate of thiazolidine ring during fragmentation of penicillin by exocellular DD-carboxypeptidase-transpeptidase of Streptomyces R61.

Authors:  J Frere; J Ghuysen; H Vanderhaeghe; P Adriaens; J Degelaen; J De Graeve
Journal:  Nature       Date:  1976-04-01       Impact factor: 49.962

8.  NMR evidence for the structure of the complex between penicillin and the DD-carboxypeptidase of Streptomyces R61.

Authors:  J Degelaen; J Feeney; G C Roberts; A S Burgen; J M Frère; J M Ghuysen
Journal:  FEBS Lett       Date:  1979-02-01       Impact factor: 4.124

9.  Binding of beta-lactam antibiotics to the exocellular DD-carboxypeptidase-transpeptidase of Streptomyces R39.

Authors:  J M Frère; J M Ghuysen; P E Reynolds; R Moreno
Journal:  Biochem J       Date:  1974-10       Impact factor: 3.857

10.  Isolation of the penicillin-binding peptide from D-alanine carboxypeptidase of Bacillus subtilis.

Authors:  N Georgopapadakou; S Hammarström; J L Strominger
Journal:  Proc Natl Acad Sci U S A       Date:  1977-03       Impact factor: 11.205
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  9 in total

1.  Cloning and amplified expression in Streptomyces lividans of the gene encoding the extracellular beta-lactamase of Actinomadura R39.

Authors:  C Piron-Fraipont; C Duez; A Matagne; C Molitor; J Dusart; J M Frère; J M Ghuysen
Journal:  Biochem J       Date:  1989-09-15       Impact factor: 3.857

2.  Active sites of beta-lactamases. The chromosomal beta-lactamases of Pseudomonas aeruginosa and Escherichia coli.

Authors:  V Knott-Hunziker; S Petursson; G S Jayatilake; S G Waley; B Jaurin; T Grundström
Journal:  Biochem J       Date:  1982-03-01       Impact factor: 3.857

3.  Primary and predicted secondary structures of the Actinomadura R39 extracellular DD-peptidase, a penicillin-binding protein (PBP) related to the Escherichia coli PBP4.

Authors:  B Granier; C Duez; S Lepage; S Englebert; J Dusart; O Dideberg; J Van Beeumen; J M Frère; J M Ghuysen
Journal:  Biochem J       Date:  1992-03-15       Impact factor: 3.857

4.  Interactions between non-classical beta-lactam compounds and the beta-lactamases of Actinomadura R39 and Streptomyces albus G.

Authors:  J A Kelly; J M Frère; C Duez; J M Ghuysen
Journal:  Biochem J       Date:  1981-10-01       Impact factor: 3.857

5.  Delta 2- and delta 3-cephalosporins, penicillinate and 6-unsubstituted penems. Intrinsic reactivity and interaction with beta-lactamases and D-alanyl-D-alanine-cleaving serine peptidases.

Authors:  J M Frère; J A Kelly; D Klein; J M Ghuysen; P Claes; H Vanderhaeghe
Journal:  Biochem J       Date:  1982-04-01       Impact factor: 3.857

6.  Sequences of the active-site peptides of three of the high-Mr penicillin-binding proteins of Escherichia coli K-12.

Authors:  W Keck; B Glauner; U Schwarz; J K Broome-Smith; B G Spratt
Journal:  Proc Natl Acad Sci U S A       Date:  1985-04       Impact factor: 11.205

7.  The Streptomyces K15 DD-peptidase/penicillin-binding protein. Active site and sequence of the N-terminal region.

Authors:  M Leyh-Bouille; J Van Beeumen; S Renier-Pirlot; B Joris; M Nguyen-Distèche; J M Ghuysen
Journal:  Biochem J       Date:  1989-06-01       Impact factor: 3.857

8.  Interaction between non-classical beta-lactam compounds and the Zn2+-containing G and serine R61 and R39 D-alanyl-D-alanine peptidases.

Authors:  J A Kelly; J M Frère; D Klein; J M Ghuysen
Journal:  Biochem J       Date:  1981-10-01       Impact factor: 3.857

9.  Production of thiol-penicillin-binding protein 3 of Escherichia coli using a two primer method of site-directed mutagenesis.

Authors:  J K Broome-Smith; P J Hedge; B G Spratt
Journal:  EMBO J       Date:  1985-01       Impact factor: 11.598
  9 in total

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