Literature DB >> 7305934

A novel procedure for the rapid purification of plastocyanin from pea (Pisum sativum) leaves.

A R Ashton, L E Anderson.   

Abstract

Plastocyanin is soluble at high concentrations (greater than 3 M) of (NH4)2SO4 but under these conditions will adsorb tightly to unsubstituted Sepharose beads. This observation was utilized to purify plastocyanin from pea (Pisum sativum) in two chromatographic steps. Sepharose-bound plastocyanin was eluted with low-ionic-strength buffer and subsequently purified to homogeneity by DEAE-cellulose chromatography.

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Year:  1981        PMID： 7305934      PMCID： PMC1162611          DOI： 10.1042/bj1930375

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  9 in total

1.  The isolation of spinach chloroplasts in pyrophosphate media.

Authors:  W Cockburn; D A Walker; C W Baldry
Journal:  Plant Physiol       Date:  1968-09       Impact factor: 8.340

2.  Photosynthetic Electron Transport Chain of Chlamydomonas reinhardi. IV. Purification and Properties of Plastocyanin.

Authors:  D S Gorman; R P Levine
Journal:  Plant Physiol       Date:  1966-12       Impact factor: 8.340

Review 3.  The Twelfth Hopkins Memorial Lecture. Molecular-sieving and non-ionic adsorption in polysaccharide gels.

Authors:  J Porath
Journal:  Biochem Soc Trans       Date:  1979-12       Impact factor: 5.407

4.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

5.  Interaction of plastocyanin and P700 in PSI reaction center particles from C. reinhardi and spinach.

Authors:  S Lien; A San Pietro
Journal:  Arch Biochem Biophys       Date:  1979-04-15       Impact factor: 4.013

6.  Higher plant plastocyanin.

Authors:  J A Ramshaw; R H Brown; M D Scawen; D Boulter
Journal:  Biochim Biophys Acta       Date:  1973-04-20

7.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

8.  Structural and molecular weight studies on the small copper protein, plastocyanin.

Authors:  P R Milne; J R Wells
Journal:  J Biol Chem       Date:  1970-04-10       Impact factor: 5.157

9.  Chromatography in presence of high concentrations of salts on columns of celluloses with and without ion exchange groups (hydrogen bond chromatography). Its application to purification of yeast enzymes.

Authors:  T Fujita; Y Suzuki; J Yamauti; I Takagahara; K Fujii; J Yamashita; T Horio
Journal:  J Biochem       Date:  1980-01       Impact factor: 3.387
  9 in total
  2 in total

1.  Regulation by copper of the expression of plastocyanin and cytochrome c552 in Chlamydomonas reinhardi.

Authors:  S Merchant; L Bogorad
Journal:  Mol Cell Biol       Date:  1986-02       Impact factor: 4.272

2.  Copper-binding proteins and copper tolerance in Pisam sativum L. : Characterization of low-molecular-weight metalloproteins from plants with different sensitivity to copper.

Authors:  J M Palma; J Yáñez; M Gómez; L A Del Río
Journal:  Planta       Date:  1990-07       Impact factor: 4.116
  2 in total

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