Literature DB >> 7305933

Double-label reductive methylation of tissue proteins for precision two-dimensional polyacrylamide-gel electrophoretic analysis.

J M Finger, K H Choo.   

Abstract

Reductive methylation has been used to radioactively label crude-extract proteins with 3H or 14C. The procedure achieved good isotope incorporation and resolution of proteins on two-dimensional polyacrylamide gels. It allows high-precision comparison of tissue samples by double-labelling and should facilitate the study of tissue proteins by two-dimensional gel electrophoresis.

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Year:  1981        PMID： 7305933      PMCID： PMC1162610          DOI： 10.1042/bj1930371

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  9 in total

1.  The tritium labeling of small amounts of protein for analysis by electrophoresis on sodium dodecyl sulfate--polyacrylamide slab gels.

Authors:  R Kumarasamy; R H Symons
Journal:  Anal Biochem       Date:  1979-06       Impact factor: 3.365

2.  High resolution two-dimensional electrophoresis of proteins.

Authors:  P H O'Farrell
Journal:  J Biol Chem       Date:  1975-05-25       Impact factor: 5.157

3.  A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.

Authors:  W M Bonner; R A Laskey
Journal:  Eur J Biochem       Date:  1974-07-01

4.  Radioactive labeling of proteins in vitro.

Authors:  R H Rice; G E Means
Journal:  J Biol Chem       Date:  1971-02-10       Impact factor: 5.157

5.  Reductive alkylation of amino groups in proteins.

Authors:  G E Means; R E Feeney
Journal:  Biochemistry       Date:  1968-06       Impact factor: 3.162

6.  Radiolabeling of proteins by reductive alkylation with [14C]formaldehyde and sodium cyanoborohydride.

Authors:  D Dottavio-Martin; J M Ravel
Journal:  Anal Biochem       Date:  1978-07-01       Impact factor: 3.365

7.  Double-labeling and high precision comparison of complex protein patterns on two-dimensional polyacrylamide gels.

Authors:  K H Choo; R G Cotton; D M Danks
Journal:  Anal Biochem       Date:  1980-03-15       Impact factor: 3.365

8.  Double-label autoradiography for comparison of complex protein mixtures after gel electrophoresis.

Authors:  E H McConkey
Journal:  Anal Biochem       Date:  1979-07-01       Impact factor: 3.365

9.  Genetics of the mammalian phenylalanine hydroxylase system. Studies of human liver phenylalanine hydroxylase subunit structure and of mutations in phenylketonuria.

Authors:  K H Choo; R G Cotton; D M Danks; I G Jennings
Journal:  Biochem J       Date:  1979-08-01       Impact factor: 3.857
  9 in total
  3 in total

1.  Functional analysis of the effect of monoclonal antibodies on monkey liver phenylalanine hydroxylase.

Authors:  I G Jennings; R G Russell; W L Armarego; R G Cotton
Journal:  Biochem J       Date:  1986-04-01       Impact factor: 3.857

Review 2.  Review of methods to assign the nuclear magnetic resonance peaks of reductively methylated proteins.

Authors:  Kevin J Roberson; Megan A Macnaughtan
Journal:  Anal Biochem       Date:  2014-08-29       Impact factor: 3.365

3.  Identification of two molecular-mass forms of phenylalanine hydroxylase that segregate independently in rats. Specific association of each form with certain rat strains.

Authors:  J F Mercer; A Grimes; I Jennings; R G Cotton
Journal:  Biochem J       Date:  1984-05-01       Impact factor: 3.857
  3 in total

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