Literature DB >> 728077

Interference by branched-chain amino acids in the assay of alanine with alanine dehydrogenase.

P Lund, G Baverel.   

Abstract

Commercial preparations of alanine dehydrogenase from Bacillus subtilis are contaminated to varying extents with activity towards branched-chain amino acids. The Km values for these amino acids are of the same order as for L-alanine (about 10(-3)M). The branched-chain amino acid dehydrogenase activity is lost on dialysis for 2--4h against water or 2mM-EDTA.

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Year:  1978        PMID： 728077      PMCID： PMC1186017          DOI： 10.1042/bj1741079

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  12 in total

Review 1.  Muscle amino acid metabolism and gluconeogenesis.

Authors:  N B Ruderman
Journal:  Annu Rev Med       Date:  1975       Impact factor: 13.739

2.  Aspects of the regulation of the metabolism of branched-chain amino acids.

Authors:  H A Krebs; P Lund
Journal:  Adv Enzyme Regul       Date:  1976

3.  ENZYMATIC PROPERTIES OF ALANINE DEHYDROGENASE OF BACILLUS SUBTILIS.

Authors:  A YOSHIDA; E FREESE
Journal:  Biochim Biophys Acta       Date:  1965-02-22

4.  Enzymatic preparation of alpha-keto acids.

Authors:  A MEISTER
Journal:  J Biol Chem       Date:  1952-05       Impact factor: 5.157

5.  The formation of alanine from amino acids in diaphragm muscle of the rat.

Authors:  L Goldstein; E A Newsholme
Journal:  Biochem J       Date:  1976-02-15       Impact factor: 3.857

6.  Amino acid metabolism in skeletal muscle. Regulation of glutamine and alanine release in the perfused rat hindquarter.

Authors:  N B Ruderman; P Lund
Journal:  Isr J Med Sci       Date:  1972-03

7.  Origin and possible significance of alanine production by skeletal muscle.

Authors:  R Odessey; E A Khairallah; A L Goldberg
Journal:  J Biol Chem       Date:  1974-12-10       Impact factor: 5.157

8.  The formation of glutamine and alanine in skeletal muscle.

Authors:  N B Ruderman; M Berger
Journal:  J Biol Chem       Date:  1974-09-10       Impact factor: 5.157

9.  The release of alanine by rat diaphragm muscle in vitro.

Authors:  K Snell; D A Duff
Journal:  Biochem J       Date:  1977-02-15       Impact factor: 3.857

10.  Amino acid balance across tissues of the forearm in postabsorptive man. Effects of insulin at two dose levels.

Authors:  T Pozefsky; P Felig; J D Tobin; J S Soeldner; G F Cahill
Journal:  J Clin Invest       Date:  1969-12       Impact factor: 14.808
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  1 in total

1.  Enzymic determination of branched-chain amino acids and 2-oxoacids in rat tissues. Transfer of 2-oxoacids from skeletal muscle to liver in vivo.

Authors:  G Livesey; P Lund
Journal:  Biochem J       Date:  1980-06-15       Impact factor: 3.857
  1 in total

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