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Literature DB >> 7278520

High performance reversed phase chromatography of cholesterol and cholesteryl esters of human plasma lipoproteins.

E G Perkins, D J Hendren, J E Bauer, A H El-Hamdy.

Abstract

Cholesterol and cholesteryl esters were separated according to their carbon number and number of double bonds by high performance reversed-phase chromatography (HPRC) using acetonitrile/chloroform/methanol (1:1:1, v/v) as a mobile phase. It was found that within the same equivalent carbon number (ECN) category, cholesteryl esters with the highest number of double bonds eluted ahead of those with a lower number of double bonds, and with the cis isomers eluting ahead of their trans partners. Thus, cholesteryl oleate (C27-18:1c) elutes ahead of cholesteryl palmitate (C27-16:0) and ahead of cholesteryl elaidate (C27-18:1t). Human lipoprotein, as well as rat liver cholesteryl esters, were separated using this technique.

Entities: Chemical Species

Mesh：

Substances：

Year: 1981 PMID： 7278520 DOI： 10.1007/BF02534908

Source DB: PubMed Journal: Lipids ISSN： 0024-4201 Impact factor: 1.880

14 in total

1. Determination of cholesterol using o-phthalaldehyde.

Authors: L L Rudel; M D Morris
Journal: J Lipid Res Date: 1973-05 Impact factor: 5.922

2. FRACTIONATION OF CHOLESTEROL ESTERS BY THIN-LAYER CHROMATOGRAPHY.

Authors: L J MORRIS
Journal: J Lipid Res Date: 1963-07 Impact factor: 5.922

3. The separation of complex lipide mixtures by the use of silicic acid chromatography.

Authors: J HIRSCH; E H AHRENS
Journal: J Biol Chem Date: 1958-08 Impact factor: 5.157

4. Disruption of low- and high-density human plasma lipoproteins and phospholipid dispersions by 1-anilinonaphthalene-8-sulfonate.

Authors: R A Muesing; T Nishida
Journal: Biochemistry Date: 1971-07-20 Impact factor: 3.162

5. A versatile lipophilic Sephadex derivative for "reversed-phase" chromatography.

Authors: J Ellingboe; E Nyström; J Sjövall
Journal: Biochim Biophys Acta Date: 1968-07-01

6. Evaluation of a high-performance liquid chromatography method for isolation and quantitation of cholesterol and cholesteryl esters.

Authors: R M Carroll; L L Rudel
Journal: J Lipid Res Date: 1981-02 Impact factor: 5.922

7. Procedure for determination of free and total cholesterol in micro- or nanogram amounts suitable for studies with cultured cells.

Authors: W Gamble; M Vaughan; H S Kruth; J Avigan
Journal: J Lipid Res Date: 1978-11 Impact factor: 5.922

High performance reversed phase chromatography of cholesterol and cholesteryl esters of human plasma lipoproteins.

1. Determination of cholesterol using o-phthalaldehyde.

2. FRACTIONATION OF CHOLESTEROL ESTERS BY THIN-LAYER CHROMATOGRAPHY.

3. The separation of complex lipide mixtures by the use of silicic acid chromatography.

4. Disruption of low- and high-density human plasma lipoproteins and phospholipid dispersions by 1-anilinonaphthalene-8-sulfonate.

5. A versatile lipophilic Sephadex derivative for "reversed-phase" chromatography.

6. Evaluation of a high-performance liquid chromatography method for isolation and quantitation of cholesterol and cholesteryl esters.

7. Procedure for determination of free and total cholesterol in micro- or nanogram amounts suitable for studies with cultured cells.

8. Gas chromatographic separation of cholesteryl esters of fatty acids of different degrees of unsaturation.

9. Liver lipid alterations in rats fed arginine deficient diets.

Review 10. Cholesterol methodology for human studies.

1. Comparative study of methods for measuring cholesterol in biological fluids.

2. High performance reversed-phase chromatography of the triglycerides from human plasma lipoproteins.

3. Plasma lipid profiling by liquid chromatography with chloride-attachment mass spectrometry.