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Literature DB >> 14580182

Determination of cholesterol using o-phthalaldehyde.

Abstract

A simple, rapid method for the determination of cholesterol in plasma and tissue using o-phthalaldehyde is presented. Comparison of this method with the FeCl(3) method gave identical results. However, the o-phthalaldehyde determination is three times more sensitive than the FeCl(3) determination (molar extinction coefficients of 11,610 and 33,440 for FeCl(3) and o-phthalaldehyde, respectively), it takes less time to complete, and the color developed is more stable. The o-phthalaldehyde method can be used to assay free and esterified cholesterol directly after thin-layer chromatographic separation.

Entities: Chemical

Year: 1973 PMID： 14580182

Source DB: PubMed Journal: J Lipid Res ISSN： 0022-2275 Impact factor: 5.922

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Cited

158 in total

1. Relative concentrations of 14C-DDT and of two polychlorinated biphenyls in the lipids of cod tissues after a single oral dose.

Authors: A I Mitchell; P A Plack; I M Thomson
Journal: Arch Environ Contam Toxicol Date: 1977 Impact factor: 2.804

2. Determination of transmembrane pH gradients and membrane potentials in liposomes.

Authors: P R Harrigan; M J Hope; T E Redelmeier; P R Cullis
Journal: Biophys J Date: 1992-11 Impact factor: 4.033

3. Regulation of aldosterone secretion by the renin-angiotensin system during sodium restriction in rats.

Authors: G Aguilera; K J Catt
Journal: Proc Natl Acad Sci U S A Date: 1978-08 Impact factor: 11.205

4. A monocarboxylate transporter required for hepatocyte secretion of ketone bodies during fasting.

Authors: Sarah E Hugo; Lourdes Cruz-Garcia; Santhosh Karanth; Ryan M Anderson; Didier Y R Stainier; Amnon Schlegel
Journal: Genes Dev Date: 2012-02-01 Impact factor: 11.361

5. Characterization of an HL-60 cell variant resistant to the antineoplastic ether lipid 1-O-octadecyl-2-O-methyl-rac-glycero-3-phosphocholine.

Authors: G W Small; J C Strum; L W Daniel
Journal: Lipids Date: 1997-07 Impact factor: 1.880

Determination of cholesterol using o-phthalaldehyde.

1. Relative concentrations of 14C-DDT and of two polychlorinated biphenyls in the lipids of cod tissues after a single oral dose.

2. Determination of transmembrane pH gradients and membrane potentials in liposomes.

3. Regulation of aldosterone secretion by the renin-angiotensin system during sodium restriction in rats.

4. A monocarboxylate transporter required for hepatocyte secretion of ketone bodies during fasting.

5. Characterization of an HL-60 cell variant resistant to the antineoplastic ether lipid 1-O-octadecyl-2-O-methyl-rac-glycero-3-phosphocholine.

6. Low-density lipoprotein as a transporter of dolichol intermediates in the mammalian circulation.

7. Chylomicron-remnant uptake by freshly isolated hepatocytes. Effect of heparin and of hepatic triacylglycerol lipase.

8. Thermal stability of Torpedo californica acetylcholine receptor in a cholesterol lipid environment.

9. Platelet hypersensitivity induced by cholesterol incorporation.

10. The effect of skim milk on plasma cholesterol in rats.