Literature DB >> 724507

A new RNA-RNA crosslinking reagent and its application to ribosomal 5S RNA.

R Wagner, R A Garrett.   

Abstract

The synthesis of a new RNA specific bifunctional crosslinking reagent, 1.4-phenyl-diglyoxal, is described which reacts exclusively with guanosines. The properties of the crosslinked products enabled us to develop a straightforward method for identifying the reacted nucleotides. Results obtained with ribosomal 5S RNA of Escherichia coli demonstrate the formation of an intramolecular crosslink between guanosine-2 and guanosine-112 in the stem region.

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Year:  1978        PMID： 724507      PMCID： PMC342734          DOI： 10.1093/nar/5.11.4065

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  16 in total

1.  On the covalent binding of mRNA models to the part of the 16 S RNA which is located in the mRNA binding site of the 30 S ribosome.

Authors:  R Wagner; H G Gassen
Journal:  Biochem Biophys Res Commun       Date:  1975-07-22       Impact factor: 3.575

2.  Chemical evidence for a codon-induced change of tRNA conformation.

Authors:  R Wagner; R A Garrett
Journal:  FEBS Lett       Date:  1978-01-15       Impact factor: 4.124

3.  Accessibility of Escherichia coli 5S RNA base residues to chemical reagents. Influence of chemical alterations on the affinity of 5S RNA for the 50S subunit structure.

Authors:  G Bellemare; B R Jordan; J Rocca-Serra; R Monier
Journal:  Biochimie       Date:  1972       Impact factor: 4.079

4.  The use of a new series of cleavable protein-crosslinkers on the Escherichia coli ribosome.

Authors:  L C Lutter; F Ortanderl; H Fasold
Journal:  FEBS Lett       Date:  1974-11-15       Impact factor: 4.124

5.  Nucleotide sequences from specific areas of the 16S and 23S ribosomal RNAs of E. coli.

Authors:  P Fellner
Journal:  Eur J Biochem       Date:  1969-11

6.  Structure of psoralen-crosslinked ribosomal RNA from Drosophila melanogaster.

Authors:  P L Wollenzien; D C Youvan; J E Hearst
Journal:  Proc Natl Acad Sci U S A       Date:  1978-04       Impact factor: 11.205

7.  Sequence-specific crosslinking agents for nucleic acids. Use of 6-bromo-5,5-dimethoxyhexanohydrazide for crosslinking cytidine to guanosine and crosslinking RNA to complementary sequences of DNA.

Authors:  J Summerton; P A Bartlett
Journal:  J Mol Biol       Date:  1978-06-25       Impact factor: 5.469

8.  Photochemical cross-linking of DNA-RNA helices by psoralen derivatives.

Authors:  C K Shen; T S Hsieh; J C Wang; J E Hearst
Journal:  J Mol Biol       Date:  1977-11       Impact factor: 5.469

9.  Chemical cross-linking of protein to RNA within intact ribosomal subunits from Escherichia coli.

Authors:  E Ulmer; M Meinke; A Ross; G Fink; R Brimacombe
Journal:  Mol Gen Genet       Date:  1978-04-06

10.  Crosslinking with bifunctional reagents as a means for studying the symmetry of oligomeric proteins.

Authors:  J Hajdu; F Bartha; P Friedrich
Journal:  Eur J Biochem       Date:  1976-09-15
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  14 in total

1.  Nested Arg-specific bifunctional crosslinkers for MS-based structural analysis of proteins and protein assemblies.

Authors:  Qingrong Zhang; Elizabeth Crosland; Daniele Fabris
Journal:  Anal Chim Acta       Date:  2008-06-05       Impact factor: 6.558

2.  Analysis of a sequence region of 5S RNA from E. coli cross-linked in situ to the ribosomal protein L25.

Authors:  C Szymkowiak; R Wagner
Journal:  Nucleic Acids Res       Date:  1985-06-11       Impact factor: 16.971

Review 3.  Structure and function of ribosomal RNA.

Authors:  R Brimacombe; W Stiege
Journal:  Biochem J       Date:  1985-07-01       Impact factor: 3.857

4.  A proton-coupled conformational switch of Escherichia coli 5S ribosomal RNA.

Authors:  T H Kao; D M Crothers
Journal:  Proc Natl Acad Sci U S A       Date:  1980-06       Impact factor: 11.205

5.  Reconstitution of biologically active 50S ribosomal subunits with artificial 5S RNA molecules carrying disturbances in the base pairing within the molecular stalk.

Authors:  H A Raué; S Lorenz; V A Erdmann; R J Planta
Journal:  Nucleic Acids Res       Date:  1981-03-11       Impact factor: 16.971

6.  Improved methods for structure probing in large RNAs: a rapid 'heterologous' sequencing approach is coupled to the direct mapping of nuclease accessible sites. Application to the 5' terminal domain of eukaryotic 28S rRNA.

Authors:  H L Qu; B Michot; J P Bachellerie
Journal:  Nucleic Acids Res       Date:  1983-09-10       Impact factor: 16.971

7.  A structural model of 5S RNA from E. coli based on intramolecular crosslinking evidence.

Authors:  J Hancock; R Wagner
Journal:  Nucleic Acids Res       Date:  1982-02-25       Impact factor: 16.971

8.  Localisation of a series of intra-RNA cross-links in 16S RNA, induced by ultraviolet irradiation of Escherichia coli 30S ribosomal subunits.

Authors:  C Zwieb; R Brimacombe
Journal:  Nucleic Acids Res       Date:  1980-06-11       Impact factor: 16.971

9.  Precise localisation of three intra-RNA cross-links in 23S RNA and one in 5S RNA, induced by treatment of Escherichia coli 50S ribosomal subunits with bis-(2-chloroethyl)-methylamine.

Authors:  W Stiege; C Zwieb; R Brimacombe
Journal:  Nucleic Acids Res       Date:  1982-11-25       Impact factor: 16.971

10.  Three-dimensional structural model of eubacterial 5S RNA that has functional implications.

Authors:  T Pieler; V A Erdmann
Journal:  Proc Natl Acad Sci U S A       Date:  1982-08       Impact factor: 11.205
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