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Literature DB >> 7041089

A structural model of 5S RNA from E. coli based on intramolecular crosslinking evidence.

Abstract

We describe new results obtained using the bifunctional chemical reagent phenyldiglyoxal (PDG) to study the intramolecular crosslinking of ribosomal 5S RNA from E. coli. In a previous publication (Wagner & Garrett [1]) we reported the identification of a crosslink in the stem region of 5S RNA (G2-G112) using the same reagent but were unable to obtain further information because of the presence of monofunctional adducts which confused the analyses. To overcome this problem, we have removed the monoaddition products by coupling them via their free reagent ends to a solid support bearing reactive groups. Using this system we have been able to identify a new crosslink G41-G72 in native 5S RNA which has considerable structural implications. We propose a structural model in which the proximity of both nucleotides is maintained by secondary interactions.

Entities: Gene Species

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Year: 1982 PMID： 7041089 PMCID： PMC320523 DOI： 10.1093/nar/10.4.1257

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

22 in total

1. Selective reaction of glyoxal with guanine residues in native and denatured Escherichia coli 5S RNA.

Authors: M Aubert; G Bellemare; R Monier
Journal: Biochimie Date: 1973 Impact factor: 4.079

2. The sequence of 5 s ribosomal ribonucleic acid.

Authors: G G Brownlee; F Sanger; B G Barrell
Journal: J Mol Biol Date: 1968-06-28 Impact factor: 5.469

3. Studies on 5 s RNA conformation by partial ribonuclease hydrolysis.

Authors: B R Jordan
Journal: J Mol Biol Date: 1971-02-14 Impact factor: 5.469

4. The function of pseudouridylic acid in transfer ribonucleic acid. II. Inhibition of amino acyl transfer ribonucleic acid-ribosome complex formation by ribothymidylyl-pseudouridylyl-cytidylyl-guanosine 3'-phosphate.

Authors: J Ofengand; C Henes
Journal: J Biol Chem Date: 1969-11-25 Impact factor: 5.157

5. A possible role for 5 S rRNA as a bridge between ribosomal subunits.

Authors: A A Azad; B G Lane
Journal: Can J Biochem Date: 1973-12

6. Structural studies on transfer ribonucleic acid. I. Labeling of exposed guanine sites in yeast phenylalanine transfer ribonucleic acid with kethoxal.

Authors: M Litt
Journal: Biochemistry Date: 1969-08 Impact factor: 3.162

7. Does 5S RNA function by a switch between two secondary structures?

Authors: H Weidner; R Yuan; D M Crothers
Journal: Nature Date: 1977-03-10 Impact factor: 49.962

8. 5S RNA secondary structure.

Authors: G E Fox; C R Woese
Journal: Nature Date: 1975-08-07 Impact factor: 49.962

Review 9. Structure and function of 5S and 5.8 S RNA.

Authors: V A Erdmann
Journal: Prog Nucleic Acid Res Mol Biol Date: 1976

10. Molecular model for 5-S RNA. A small-angle x-ray scattering study of native, denatured and aggregated 5-S RNA from Escherichia coli ribosomes.

Authors: R Osterberg; B Sjöberg; R A Garrett
Journal: Eur J Biochem Date: 1976-09-15

16 in total

1. The origin of the 5S ribosomal RNA molecule could have been caused by a single inverse duplication: strong evidence from its sequences.

Authors: Sergio Branciamore; Massimo Di Giulio
Journal: J Mol Evol Date: 2012-04-11 Impact factor: 2.395

10. The secondary structure of oocyte and somatic 5S ribosomal RNAs of the fish Misgurnus fossilis L. from nuclease hydrolyses and chemical modification data.

Authors: T I Serenkova; A M Mazo; T D Mashkova; I Toots; A Nigul; L L Kisselev
Journal: Nucleic Acids Res Date: 1984-07-11 Impact factor: 16.971

A structural model of 5S RNA from E. coli based on intramolecular crosslinking evidence.

1. Selective reaction of glyoxal with guanine residues in native and denatured Escherichia coli 5S RNA.

2. The sequence of 5 s ribosomal ribonucleic acid.

3. Studies on 5 s RNA conformation by partial ribonuclease hydrolysis.

4. The function of pseudouridylic acid in transfer ribonucleic acid. II. Inhibition of amino acyl transfer ribonucleic acid-ribosome complex formation by ribothymidylyl-pseudouridylyl-cytidylyl-guanosine 3'-phosphate.

5. A possible role for 5 S rRNA as a bridge between ribosomal subunits.

6. Structural studies on transfer ribonucleic acid. I. Labeling of exposed guanine sites in yeast phenylalanine transfer ribonucleic acid with kethoxal.

7. Does 5S RNA function by a switch between two secondary structures?

8. 5S RNA secondary structure.

Review 9. Structure and function of 5S and 5.8 S RNA.

10. Molecular model for 5-S RNA. A small-angle x-ray scattering study of native, denatured and aggregated 5-S RNA from Escherichia coli ribosomes.

1. The origin of the 5S ribosomal RNA molecule could have been caused by a single inverse duplication: strong evidence from its sequences.

2. A model of the origin of the 5S ribosomal RNA molecule.

3. Does 5S RNA from E. coli have a pseudoknotted structure?

4. The evolutionary history of the structure of 5S ribosomal RNA.

5. The environment of 5S rRNA in the ribosome: cross-links to the GTPase-associated area of 23S rRNA.

6. Evolutionary changes in the higher order structure of the ribosomal 5S RNA.

7. Analysis of a sequence region of 5S RNA from E. coli cross-linked in situ to the ribosomal protein L25.

Review 8. Structure and function of ribosomal RNA.

9. The effects of disrupting 5S RNA helical structures on the binding of Xenopus transcription factor IIIA.

10. The secondary structure of oocyte and somatic 5S ribosomal RNAs of the fish Misgurnus fossilis L. from nuclease hydrolyses and chemical modification data.