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Literature DB >> 6181508

Three-dimensional structural model of eubacterial 5S RNA that has functional implications.

Abstract

Escherichia coli 5S RNA and its specific protein complexes were hydrolyzed with the single-strand-specific nuclease S1. Based on the results, a tertiary structural model for E. coli 5S RNA is proposed in which ribosomal proteins E-L5, E-L18, and E-L25 influence the conformation of the RNA. This may be of significance for ribosomal function. Comparison of the proposed E. coli 5S RNA structure with those of 18 other prokaryotic 5S RNAs led to a generalized eubacterial 5S RNA tertiary structure in which the majority of the conserved nucleotides are in non-base-paired regions and several conserved "looped-out" adenines (in E. coli, adenines -52, -53, -57, -58, and -66) are implied to be important for protein recognition or interaction or both.

Entities: Chemical Gene Species

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Year: 1982 PMID： 6181508 PMCID： PMC346722 DOI： 10.1073/pnas.79.15.4599

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

35 in total

1. Micro thin-layer techniques for rapid sequence analysis of 32P-labeled RNA: double digestion and pancreatic ribonuclease analyses.

Authors: G Volckaert; W Fiers
Journal: Anal Biochem Date: 1977-11 Impact factor: 3.365

2. Stoichiometry, cooperativity, and stability of interactions between 5S RNA and proteins L5, L18, and L25 from the 50S ribosomal subunit of Escherichia coli.

Authors: P Spierer; R A Zimmermann
Journal: Biochemistry Date: 1978-06-27 Impact factor: 3.162

3. A ribonuclease-resistant region of 5S RNA and its relation to the RNA binding sites of proteins L18 and L25.

Authors: S Douthwaite; R A Garrett; R Wagner; J Feunteun
Journal: Nucleic Acids Res Date: 1979-06-11 Impact factor: 16.971

4. Identification of the single-strand regions in Escherichia coli 5S RNA, native and A forms, by the binding of oligonucleotides.

Authors: J B Lewis; P Doty
Journal: Biochemistry Date: 1977-11-15 Impact factor: 3.162

5. Binding oligonucleotides to Escherichia coli and Bacillus stearothermophilus 5 S RNA.

Authors: P Wrede; O Pongs; V A Erdmann
Journal: J Mol Biol Date: 1978-03-25 Impact factor: 5.469

6. Evolutionary change in 5S RNA secondary structure and a phylogenic tree of 54 5S RNA species.

Authors: H Hori; S Osawa
Journal: Proc Natl Acad Sci U S A Date: 1979-01 Impact factor: 11.205

7. A new RNA-RNA crosslinking reagent and its application to ribosomal 5S RNA.

Authors: R Wagner; R A Garrett
Journal: Nucleic Acids Res Date: 1978-11 Impact factor: 16.971

8. Binding sites of E. coli and B. stearothermophilus ribosomal proteins on B stearothermophilus 5S RNA.

Authors: J Zimmermann; V A Erdmann
Journal: Nucleic Acids Res Date: 1978-07 Impact factor: 16.971

9. Identification of Escherichia coli and Bacillus stearothermophilus ribosomal protein binding sites on Escherichia coli 5S RNA.

Authors: J Zimmermann; V A Erdmann
Journal: Mol Gen Genet Date: 1978-04-17

10. Structure and function of 5S ribosomal ribonucleic acid from Torulopsis utilis. III. Detection of single-stranded regions by digestion with nuclease S1.

Authors: K Nishikawa; S Takemura
Journal: J Biochem Date: 1977-04 Impact factor: 3.387

24 in total

1. Detection of multiple conformations of the E-domain of 5S rRNA from Escherichia coli in solution and in crystals by NMR spectroscopy.

Authors: M Grüne; J P Fürste; S Klussmann; V A Erdmann; L R Brown
Journal: Nucleic Acids Res Date: 1996-07-01 Impact factor: 16.971

Three-dimensional structural model of eubacterial 5S RNA that has functional implications.

1. Micro thin-layer techniques for rapid sequence analysis of 32P-labeled RNA: double digestion and pancreatic ribonuclease analyses.

2. Stoichiometry, cooperativity, and stability of interactions between 5S RNA and proteins L5, L18, and L25 from the 50S ribosomal subunit of Escherichia coli.

3. A ribonuclease-resistant region of 5S RNA and its relation to the RNA binding sites of proteins L18 and L25.

4. Identification of the single-strand regions in Escherichia coli 5S RNA, native and A forms, by the binding of oligonucleotides.

5. Binding oligonucleotides to Escherichia coli and Bacillus stearothermophilus 5 S RNA.

6. Evolutionary change in 5S RNA secondary structure and a phylogenic tree of 54 5S RNA species.

7. A new RNA-RNA crosslinking reagent and its application to ribosomal 5S RNA.

8. Binding sites of E. coli and B. stearothermophilus ribosomal proteins on B stearothermophilus 5S RNA.

9. Identification of Escherichia coli and Bacillus stearothermophilus ribosomal protein binding sites on Escherichia coli 5S RNA.

10. Structure and function of 5S ribosomal ribonucleic acid from Torulopsis utilis. III. Detection of single-stranded regions by digestion with nuclease S1.

1. Detection of multiple conformations of the E-domain of 5S rRNA from Escherichia coli in solution and in crystals by NMR spectroscopy.

2. Does 5S RNA from E. coli have a pseudoknotted structure?

3. Nuclease S1 analysis of eubacterial 5S rRNA secondary structure.

4. Exploration of the L18 binding site on 5S RNA by deletion mutagenesis.

5. Evidence for tertiary structure in natural single stranded RNAs in solution.

Review 6. Probing the structure of RNAs in solution.

7. TFIIIA binds to different domains of 5S RNA and the Xenopus borealis 5S RNA gene.

8. Evolutionary changes in the higher order structure of the ribosomal 5S RNA.

9. Analysis of a sequence region of 5S RNA from E. coli cross-linked in situ to the ribosomal protein L25.

10. Direct 5S rRNA Assay for Monitoring Mixed-Culture Bioprocesses.