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Literature DB >> 7236247

Mitochondrial and cytosolic ATP/ADP ratios in isolated hepatocytes. A comparison of the digitonin method and the non-aqueous fractionation procedure.

S Soboll, T P Akerboom, W D Schwenke, R Haase, H Sies.

Abstract

The ratio of ATP content/ADP content in the mitochondrial matrix was found to be 2.07 +/- 0.21 and 2.26 +/- 0.22 as determined with six different preparations of isolated hepatocytes subfractionated with the digitonin and non-aqueous-fractionation procedures, respectively. In contrast, the mitochondrial matrix ATP/ADP determined with isolated haemoglobin-free perfused liver by using the non-aqueous-fractionation procedure was about 0.2, whereas the cytosolic values obtained with isolated cells and with the intact organ were similar. It is concluded that the relatively higher ATP/ADP ratio in the mitochondrial matrix of isolated hepatocytes represents a biochemical difference due to properties of the model rather than a methodological artifact.

Entities: Chemical Gene

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Year: 1980 PMID： 7236247 PMCID： PMC1162423 DOI： 10.1042/bj1920951

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

10 in total

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Authors: P F Zuurendonk; M E Tischler; T P Akerboom; R Van Der Meer; J R Williamson; J M Tager
Journal: Methods Enzymol Date: 1979 Impact factor: 1.600

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Authors: H W Heldt; M Klingenberg
Journal: Eur J Biochem Date: 1968-03

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Authors: T P Akerboom; H Bookelman; P F Zuurendonk; R van der Meer; J M Tager
Journal: Eur J Biochem Date: 1978-03-15

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Authors: R Elbers; H W Heldt; P Schmucker; S Soboll; H Wiese
Journal: Hoppe Seylers Z Physiol Chem Date: 1974-03

5. Inhibition of urea-cycle activity by high concentrations of alanine.

Authors: H E Hensgens; A J Meijer
Journal: Biochem J Date: 1980-01-15 Impact factor: 3.857

6. Mitochondrial and cytosolic NADPH systems and isocitrate dehydrogenase indicator metabolites during ureogensis from ammonia in isolated rat hepatocytes.

Authors: H Sies; T P Akerboom; J M Tager
Journal: Eur J Biochem Date: 1977-01

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Authors: S Soboll; R Scholz; H W Heldt
Journal: Eur J Biochem Date: 1978-06-15

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Authors: E A Siess; D G Brocks; O H Wieland
Journal: Hoppe Seylers Z Physiol Chem Date: 1978-07

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Authors: E A Siess; O H Wieland
Journal: Biochem J Date: 1976-04-15 Impact factor: 3.857

10. High-yield preparation of isolated rat liver parenchymal cells: a biochemical and fine structural study.

Authors: M N Berry; D S Friend
Journal: J Cell Biol Date: 1969-12 Impact factor: 10.539

10 in total

13 in total

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