Literature DB >> 7162986

A photoinduced cleavage of DNA useful for determining T residues.

A Simoncsits, I Török.   

Abstract

Irradiation of 5'-[32P]-phosphate labeled DNA fragments with ultraviolet light in the presence of primary amines followed by piperidine treatment resulted in base-specific cleavage of the DNA chain at T residues, accompanied by a less intensive G reaction. This simple, T greater than G cleavage offers an alternative method for determining T residues in chemical DNA sequencing.

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Year:  1982        PMID： 7162986      PMCID： PMC327062          DOI： 10.1093/nar/10.24.7959

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  7 in total

1.  Base-specific reactions useful for DNA sequencing: methylene blue--sensitized photooxidation of guanine and osmium tetraoxide modification of thymine.

Authors:  T Friedmann; D M Brown
Journal:  Nucleic Acids Res       Date:  1978-02       Impact factor: 16.971

2.  A new method for sequencing DNA.

Authors:  A M Maxam; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

3.  Nucleotide sequences of heat shock activated genes in Drosophila melanogaster. I. Sequences in the regions of the 5' and 3' ends of the hsp 70 gene in the hybrid plasmid 56H8.

Authors:  I Török; F Karch
Journal:  Nucleic Acids Res       Date:  1980-07-25       Impact factor: 16.971

4.  Nucleotide sequence homology at 12 intron--exon junctions in the chick ovalbumin gene.

Authors:  J F Catterall; B W O'Malley; M A Robertson; R Staden; Y Tanaka; G G Brownlee
Journal:  Nature       Date:  1978-10-12       Impact factor: 49.962

5.  Evolutionary implications of a complex pattern of DNA sequence homology extending far upstream of the hsp70 genes at loci 87A7 and 87C1 in Drosophila melanogaster.

Authors:  P J Mason; I Török; I Kiss; F Karch; A Udvardy
Journal:  J Mol Biol       Date:  1982-03-25       Impact factor: 5.469

6.  Rapid gel sequencing of RNA by primed synthesis with reverse transcriptase.

Authors:  G G Brownlee; E M Cartwright
Journal:  J Mol Biol       Date:  1977-07       Impact factor: 5.469

7.  Pyrimidine-specific chemical reactions useful for DNA sequencing.

Authors:  C M Rubin; C W Schmid
Journal:  Nucleic Acids Res       Date:  1980-10-24       Impact factor: 16.971
  7 in total
  3 in total

1.  Structure and organization of the chicken H2B histone gene family.

Authors:  D K Grandy; J B Dodgson
Journal:  Nucleic Acids Res       Date:  1987-02-11       Impact factor: 16.971

2.  A new procedure for determining thymine residues in DNA sequencing. Photoinduced cleavage of DNA fragments in the presence of spermine.

Authors:  I Saito; H Sugiyama; T Matsuura; K Ueda; T Komano
Journal:  Nucleic Acids Res       Date:  1984-03-26       Impact factor: 16.971

3.  The chicken carbonic anhydrase II gene: evidence for a recent shift in intron position.

Authors:  C M Yoshihara; J D Lee; J B Dodgson
Journal:  Nucleic Acids Res       Date:  1987-01-26       Impact factor: 16.971
  3 in total

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