Literature DB >> 7150233

A general method for affinity purification of complement component C3b using factor H-sepharose.

J D Scott, J E Fothergill.   

Abstract

Complement component C3b has been purified from human, rabbit and bovine serum by affinity chromatography on human factor H-Sepharose after preliminary fractionation by poly(ethylene glycol) and DEAE-Sepharose. The yields are high (35--40%) and the whole process is rapid (3 days). Binding of C3b to factor H-Sepharose is equimolar, has a sharp optimum pH at 7.6 and is quite sensitive to ionic strength.

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Year:  1982        PMID： 7150233      PMCID： PMC1158523          DOI： 10.1042/bj2050575

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  26 in total

1.  The serine protease nature of the C3 and C5 convertases of the classical and alternative complement pathways.

Authors:  R G Medicus; O Götze; H J Müller-Eberhard
Journal:  Scand J Immunol       Date:  1976       Impact factor: 3.487

2.  Modulation of C3b hemolytic activity by a plasma protein distinct from C3b inactivator.

Authors:  K Whaley; S Ruddy
Journal:  Science       Date:  1976-09-10       Impact factor: 47.728

Review 3.  A molecular approach to the complement system.

Authors:  J E Fothergill; W H Anderson
Journal:  Curr Top Cell Regul       Date:  1978

4.  Reaction mechanism of the alternative pathway of complement fixation.

Authors:  P J Lachmann; P Nicol
Journal:  Lancet       Date:  1973-03-03       Impact factor: 79.321

5.  Rocket immunoelectrophoresis.

Authors:  B Weeke
Journal:  Scand J Immunol Suppl       Date:  1973

6.  Selective enzyme purification by affinity chromatography.

Authors:  P Cuatrecasas; M Wilchek; C B Anfinsen
Journal:  Proc Natl Acad Sci U S A       Date:  1968-10       Impact factor: 11.205

7.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

8.  Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies.

Authors:  C B Laurell
Journal:  Anal Biochem       Date:  1966-04       Impact factor: 3.365

9.  Third component of human complement: purification from plasma and physicochemical characterization.

Authors:  B D Tack; J W Prahl
Journal:  Biochemistry       Date:  1976-10-05       Impact factor: 3.162

10.  Biosynthesis of pro-C3, a precursor of the third component of complement.

Authors:  V Brade; R E Hall; H R Colten
Journal:  J Exp Med       Date:  1977-09-01       Impact factor: 14.307
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  3 in total

1.  Interactions between human complement components factor H, factor I and C3b.

Authors:  C J Soames; R B Sim
Journal:  Biochem J       Date:  1997-09-01       Impact factor: 3.857

2.  The serine proteinase chain of human complement component C1s. Cyanogen bromide cleavage and N-terminal sequences of the fragments.

Authors:  P E Carter; B Dunbar; J E Fothergill
Journal:  Biochem J       Date:  1983-12-01       Impact factor: 3.857

3.  The complement system of the goat: haemolytic assays and isolation of major proteins.

Authors:  Isabel Moreno-Indias; Alister W Dodds; Anastasio Argüello; Noemi Castro; Robert B Sim
Journal:  BMC Vet Res       Date:  2012-06-26       Impact factor: 2.741
  3 in total

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