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Literature DB >> 6322105

The in vivo and in vitro initiation site for transcription of the rRNA operon of Saccharomyces carlsbergensis.

J Klootwijk, M P Verbeet, G M Veldman, V C de Regt, H van Heerikhuizen, J Bogerd, R J Planta.

Abstract

We have performed a detailed analysis of the transcription initiation of the rRNA operon in the yeast Saccharomyces carlsbergensis. Electron microscopic analysis of R-looped pre-rRNA molecules together with a very sensitive S1-nuclease mapping showed the use of only a single transcription start at about 700 bp upstream of the 17S rRNA gene and not of the minor start sites proposed for the very closely related species S. cerevisiae by others [Bayev et al. (5), Swanson and Holland (6)]. The sequence of 730 bp of the initiating region is presented. In vitro transcription in concentrated lysates of yeast spheroplasts in the presence of (gamma-SH)ATP or (gamma-SH)GTP, followed by purification of the in vitro initiated RNA via Hg-agarose, revealed that on the endogenous template exactly the same site is used for transcription initiation as in vivo.

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Year: 1984 PMID： 6322105 PMCID： PMC318583 DOI： 10.1093/nar/12.3.1377

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

21 in total

1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors: E M Southern
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

2. A new method for sequencing DNA.

Authors: A M Maxam; W Gilbert
Journal: Proc Natl Acad Sci U S A Date: 1977-02 Impact factor: 11.205

3. Incorporation of purine nucleoside 5'-[gamma-S]triphosphates as affinity probes for initiation of RNA synthesis in vitro.

Authors: A E Reeve; M M Smith; V Pigiet; R C Huang
Journal: Biochemistry Date: 1977-10-04 Impact factor: 3.162

4. Ribosomal RNA genes of Saccharomyces cerevisiae. I. Physical map of the repeating unit and location of the regions coding for 5 S, 5.8 S, 18 S, and 25 S ribosomal RNAs.

Authors: G I Bell; L J DeGennaro; D H Gelfand; R J Bishop; P Valenzuela; W J Rutter
Journal: J Biol Chem Date: 1977-11-25 Impact factor: 5.157

5. Nucleotide sequence of yeast 5 S ribosomal RNA.

Authors: J Hindley; S M Page
Journal: FEBS Lett Date: 1972-10-01 Impact factor: 4.124

6. Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event.

Authors: D B Clewell; D R Helinski
Journal: Biochemistry Date: 1970-10-27 Impact factor: 3.162

7. The derivatization of cross-linked polyacrylamide beads. Controlled introduction of functional groups for the preparation of special-purpose, biochemical adsorbents.

Authors: J K Inman; H M Dintzis
Journal: Biochemistry Date: 1969-10 Impact factor: 3.162

8. Molecular cloning of the rDNA of Saccharomyces rosei and comparison of its transcription initiation region with that of Saccharomyces carlsbergensis.

Authors: M P Verbeet; J Klootwijk; H van Heerikhuizen; R Fontijn; E Vreugdenhil; R J Planta
Journal: Gene Date: 1983-07 Impact factor: 3.688

9. Specific gene transcription in yeast nuclei and chromatin by added homologous RNA polymerases I and II.

Authors: P A Tekamp; P Valenzuela; T Maynard; G I Bell; W J Rutter
Journal: J Biol Chem Date: 1979-02-10 Impact factor: 5.157

10. Ribosomal RNA genes of Saccharomyces cerevisiae. II. Physical map and nucleotide sequence of the 5 S ribosomal RNA gene and adjacent intergenic regions.

Authors: P Valenzuela; G I Bell; A Venegas; E T Sewell; F R Masiarz; L J DeGennaro; F Weinberg; W J Rutter
Journal: J Biol Chem Date: 1977-11-25 Impact factor: 5.157

8 in total

1. Sequences within the spacer region of yeast rRNA cistrons that stimulate 35S rRNA synthesis in vivo mediate RNA polymerase I-dependent promoter and terminator activities.

Authors: R Mestel; M Yip; J P Holland; E Wang; J Kang; M J Holland
Journal: Mol Cell Biol Date: 1989-03 Impact factor: 4.272

2. Activated levels of rRNA synthesis in fission yeast are driven by an intergenic rDNA region positioned over 2500 nucleotides upstream of the initiation site.

Authors: Z Liu; A Zhao; L Chen; L Pape
Journal: Nucleic Acids Res Date: 1997-02-01 Impact factor: 16.971

The in vivo and in vitro initiation site for transcription of the rRNA operon of Saccharomyces carlsbergensis.

1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

2. A new method for sequencing DNA.

3. Incorporation of purine nucleoside 5'-[gamma-S]triphosphates as affinity probes for initiation of RNA synthesis in vitro.

4. Ribosomal RNA genes of Saccharomyces cerevisiae. I. Physical map of the repeating unit and location of the regions coding for 5 S, 5.8 S, 18 S, and 25 S ribosomal RNAs.

5. Nucleotide sequence of yeast 5 S ribosomal RNA.

6. Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event.

7. The derivatization of cross-linked polyacrylamide beads. Controlled introduction of functional groups for the preparation of special-purpose, biochemical adsorbents.

8. Molecular cloning of the rDNA of Saccharomyces rosei and comparison of its transcription initiation region with that of Saccharomyces carlsbergensis.

9. Specific gene transcription in yeast nuclei and chromatin by added homologous RNA polymerases I and II.

10. Ribosomal RNA genes of Saccharomyces cerevisiae. II. Physical map and nucleotide sequence of the 5 S ribosomal RNA gene and adjacent intergenic regions.

1. Sequences within the spacer region of yeast rRNA cistrons that stimulate 35S rRNA synthesis in vivo mediate RNA polymerase I-dependent promoter and terminator activities.

2. Activated levels of rRNA synthesis in fission yeast are driven by an intergenic rDNA region positioned over 2500 nucleotides upstream of the initiation site.

3. Deletion mapping of the yeast Pol I promoter.

4. An RNA polymerase I enhancer in Saccharomyces cerevisiae.

Review 5. Synthesis of ribosomes in Saccharomyces cerevisiae.

6. Transcription of a yeast ribosomal RNA minigene in Saccharomyces cerevisiae.

7. A new rRNA processing mutant of Saccharomyces cerevisiae.

8. Transcription of an artificial ribosomal RNA gene in yeast.