Literature DB >> 7140714

Radiochemical assay for gamma-aminolevulinate synthase.

J D Brooker, G Srivastava, B K May, W H Elliott.   

Abstract

We describe here a standard protocol for the radiochemical assay of gamma-aminolevulinate synthase from 17-day chick embryo liver mitochondria and starved male rat liver mitochondria. The assay is sensitive to at least 15 pmol of the gamma-aminolevulinate produced and can be adapted to suit most tissue types. A number of possible modifications to the procedure are discussed.

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Year:  1982        PMID： 7140714     DOI： 10.1159/000459095

Source DB:  PubMed          Journal:  Enzyme        ISSN： 0013-9432


  5 in total

1.  The effects of acute-phase inducers and dimethyl sulphoxide on delta-aminolaevulinate synthase activity in human HepG2 hepatoma cells.

Authors:  F Iwasa; S Sassa; A Kappas
Journal:  Biochem J       Date:  1989-04-15       Impact factor: 3.857

2.  delta-Aminolaevulinate synthase in human HepG2 hepatoma cells. Repression by haemin and induction by chemicals.

Authors:  F Iwasa; S Sassa; A Kappas
Journal:  Biochem J       Date:  1989-09-15       Impact factor: 3.857

3.  Measurement of 5-aminolaevulinate synthase activity by gas-liquid chromatography with electron-capture detection.

Authors:  A Gorchein
Journal:  Biochem J       Date:  1990-08-15       Impact factor: 3.857

4.  A method for determining δ-aminolevulinic acid synthase activity in homogenized cells and tissues.

Authors:  Hector A Bergonia; Michael R Franklin; James P Kushner; John D Phillips
Journal:  Clin Biochem       Date:  2015-05-08       Impact factor: 3.281

5.  Biphasic ordered induction of heme synthesis in differentiating murine erythroleukemia cells: role of erythroid 5-aminolevulinate synthase.

Authors:  H Lake-Bullock; H A Dailey
Journal:  Mol Cell Biol       Date:  1993-11       Impact factor: 4.272
  5 in total

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