Literature DB >> 2396971

Measurement of 5-aminolaevulinate synthase activity by gas-liquid chromatography with electron-capture detection.

A Gorchein1.   

Abstract

A specific assay for 5-aminolaevulinate synthase activity is described, with a sensitivity comparable with that of radiochemical assays. It is based on measurement by g.l.c. with electron-capture detection of the pentafluorobenzyl ester of the ethyl acetoacetate pyrrole derivative of 5-aminolaevulinic acid, and of the corresponding compound from 6-amino-5-oxohexanoic acid used as internal standard. Enzyme activity has been measured in homogenates of rat liver, spleen, kidney and brain, and in human lymphocytes.

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Year:  1990        PMID： 2396971      PMCID： PMC1131684          DOI： 10.1042/bj2700103

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  18 in total

1.  Delta-aminolevulinic acid synthetase assay in chicken liver homogenates and particulate fractions.

Authors:  B Yoda; B A Schacter; L G Israels
Journal:  Anal Biochem       Date:  1975-05-26       Impact factor: 3.365

2.  Biosynthesis of alpha-aminoketones and the metabolism of aminoacetone.

Authors:  G URATA; S GRANICK
Journal:  J Biol Chem       Date:  1963-02       Impact factor: 5.157

3.  Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g.

Authors:  A Böyum
Journal:  Scand J Clin Lab Invest Suppl       Date:  1968

4.  Radiochemical microassay of delta-aminolevulinic acid synthetase in hepatic and erythroid tissues.

Authors:  L J Strand; A L Swanson; J Manning; S Branch; H S Marver
Journal:  Anal Biochem       Date:  1972-06       Impact factor: 3.365

5.  Measurement of aminolaevulinate synthetase activity in normal mouse liver with [2-14C] glycine.

Authors:  R I Freshney; J Paul
Journal:  Biochim Biophys Acta       Date:  1970-12-16

6.  Studied on cerebellar haem metabolism in the rat in vivo.

Authors:  F De Matteis; D E Ray
Journal:  J Neurochem       Date:  1982-08       Impact factor: 5.372

7.  Determination of delta-aminolaevulinic acid in biological fluids by gas-liquid chromatography with electron-capture detection.

Authors:  A Gorchein
Journal:  Biochem J       Date:  1984-05-01       Impact factor: 3.857

8.  Comparisons and modifications of the colorimetric assay for delta-aminolevulinic acid synthase.

Authors:  L F Lien; D S Beattie
Journal:  Enzyme       Date:  1982

9.  delta-Aminolaevulinic acid in plasma, cerebrospinal fluid, saliva and erythrocytes: studies in normal, uraemic and porphyric subjects.

Authors:  A Gorchein; R Webber
Journal:  Clin Sci (Lond)       Date:  1987-01       Impact factor: 6.124

10.  Measurement of 5-aminolevulinic acid synthase activity in whole and fractionated human bone marrow: effect of myeloid cell lysis by monoclonal antibody.

Authors:  E J Fitzsimons; A May; G H Elder; A Jacobs
Journal:  Anal Biochem       Date:  1986-02-15       Impact factor: 3.365
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