Literature DB >> 7092832

Studies of lactate dehydrogenase in the purified state and in intact erythrocytes.

R J Simpson, K M Brindle, F F Brown, I D Campbell, D L Foxall.   

Abstract

Lactate dehydrogenase in intact erythrocytes was studied by observing isotope exchange between lactate and pyruvate by p.m.r. The inhibition of the enzyme in intact cells by both oxalate and pyruvate was found to be similar to that of the purified enzyme. The activity of the enzyme in intact cells indicates that the free solution NAD+ + NADH concentration in erythrocytes is about 10 microM whereas the total extractable NAD+ + NADH is about 80 nmol/ml of cell water.

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Year:  1982        PMID： 7092832      PMCID： PMC1158151          DOI： 10.1042/bj2020581

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  28 in total

1.  Molecular kinetics of beef heart lactate dehydrogenase.

Authors:  U Borgmann; T W Moon; K J Laidler
Journal:  Biochemistry       Date:  1974-12-03       Impact factor: 3.162

2.  [Determination of oxydated and reduced pyridine nucleotides in human and rabbit blood using the polarographic cycling method].

Authors:  I Steinbrecht; W Kunz
Journal:  Acta Biol Med Ger       Date:  1972

3.  A simple ultramicro method for determination of pyridine nucleotides in tissues.

Authors:  J S Nisselbaum; S Green
Journal:  Anal Biochem       Date:  1969-02       Impact factor: 3.365

4.  Rate of isotope exchange in enzyme-catalyzed reactions.

Authors:  G Yagil; H D Hoberman
Journal:  Biochemistry       Date:  1969-01       Impact factor: 3.162

5.  Cofactors and erythrocyte glycolytic capacity.

Authors:  K K Tsuboi; J F Allan; K Fukunaga
Journal:  J Biol Chem       Date:  1966-04-10       Impact factor: 5.157

Review 6.  Lactate dehydrogenases: structure and function.

Authors:  J Everse; N O Kaplan
Journal:  Adv Enzymol Relat Areas Mol Biol       Date:  1973

7.  Pyruvate inhibition of lactate dehydrogenase activity in human tissue extracts.

Authors:  A L Latner; S A Siddiqui; A W Skillen
Journal:  Science       Date:  1966-10-28       Impact factor: 47.728

8.  Studies on the control of lactate dehydrogenase activity in mammalian cells. II. Demonstration of rapid continuous variations in lactate dehydrogenase activity in humans erythrocytes and their relation to a biological activator and an inhibitor of lactate dehydrogenase.

Authors:  P Duffy; J C Sanderson
Journal:  Biochim Biophys Acta       Date:  1971-09-21

9.  Glyceraldehyde-3-phosphate dehydrogenase catalyzed hydration of the 5-6 double bond of reduced beta-nicotinamide adenine dinucleotide (betaNADH). Formation of beta-6-hydroxy-1,4,5,6-tetrahydronicotinamide adenine dinucleotide.

Authors:  N J Oppenheimer; N O Kaplan
Journal:  Biochemistry       Date:  1974-11-05       Impact factor: 3.162

10.  Measured and calculated NAD+-NADH ratios in human erythrocytes.

Authors:  W E Marshall; A Omachi
Journal:  Biochim Biophys Acta       Date:  1974-06-20
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  5 in total

1.  A p.m.r. isotope-exchange method for studying the kinetic properties of dehydrogenases in intact cells.

Authors:  R J Simpson; K M Brindle; F F Brown; I D Campbell; D L Foxall
Journal:  Biochem J       Date:  1982-03-15       Impact factor: 3.857

2.  A 1H n.m.r. study of the kinetic properties expressed by glyceraldehyde phosphate dehydrogenase in the intact human erythrocyte.

Authors:  K M Brindle; I D Campbell; R J Simpson
Journal:  Biochem J       Date:  1982-12-15       Impact factor: 3.857

3.  A 1H n.m.r. study of isotope exchange catalysed by glycolytic enzymes in the human erythrocyte.

Authors:  K M Brindle; F F Brown; I D Campbell; D L Foxall; R J Simpson
Journal:  Biochem J       Date:  1982-03-15       Impact factor: 3.857

Review 4.  Assessing Therapeutic Efficacy in Real-time by Hyperpolarized Magnetic Resonance Metabolic Imaging.

Authors:  Prasanta Dutta; Travis C Salzillo; Shivanand Pudakalakatti; Seth T Gammon; Benny A Kaipparettu; Florencia McAllister; Shawn Wagner; Daniel E Frigo; Christopher J Logothetis; Niki M Zacharias; Pratip K Bhattacharya
Journal:  Cells       Date:  2019-04-11       Impact factor: 6.600

5.  Assessing Metabolic Intervention with a Glutaminase Inhibitor in Real-Time by Hyperpolarized Magnetic Resonance in Acute Myeloid Leukemia.

Authors:  Niki M Zacharias; Natalia Baran; Sriram S Shanmugavelandy; Jaehyuk Lee; Juliana Velez Lujan; Prasanta Dutta; Steven W Millward; Tianyu Cai; Christopher G Wood; David Piwnica-Worms; Marina Konopleva; Pratip K Bhattacharya
Journal:  Mol Cancer Ther       Date:  2019-08-06       Impact factor: 6.261
  5 in total

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