Literature DB >> 7082299

Specificity of ovine submaxillary-gland sialyltransferases. Application of high-pressure liquid chromatography in the identification of sialo-oligosaccharide products.

M L Bergh, P L Koppen, D H Van den Eijnden.   

Abstract

High-pressure liquid chromatography was used to identify the sialo-oligosaccharide products obtained after sialylation of [14C]Gal-GalNAc-protein in vitro by an ovine submaxillary-gland microsomal fraction. Among other products, two isomeric trisaccharides could be identified. NeuAc alpha 2 leads to 3Gal beta 1 leads to 3GalNAcol and Gal beta 1 leads to 3-(NeuAc alpha 2 leads to 6)GalNAcol respectively, indicating that ovine submaxillary gland contains two sialyltransferases acting on mucin-type acceptors, a beta-galactoside alpha 2 leads to 3 sialyltransferase and a N-acetylgalactosaminide alpha 2 leads to 6 sialyltransferase. This conclusion was fully supported by methylation analysis of the two trisaccharide products.

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Year:  1982        PMID： 7082299      PMCID： PMC1163658          DOI： 10.1042/bj2010411

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  15 in total

1.  Structure and location of the O-glycosidic carbohydrate units of human chorionic gonadotropin.

Authors:  M J Kessler; T Mise; R D Ghai; O P Bahl
Journal:  J Biol Chem       Date:  1979-08-25       Impact factor: 5.157

2.  The sialic acids. XVI. Isolation of a mucin sialyltransferase from sheep submaxillary gland.

Authors:  D M Carlson; E J McGuire; G W Jourdian; S Roseman
Journal:  J Biol Chem       Date:  1973-08-25       Impact factor: 5.157

3.  A convenient method for the preparation of cytidine 5'-monophospho-N-acetylneuraminic acid.

Authors:  D H van den Eijnden; W van Dijk
Journal:  Hoppe Seylers Z Physiol Chem       Date:  1972-12

4.  Evidence for two CMP-N-acetylneuraminic acid: lactose sialyltransferases in rat, porcine, bovine, and human liver.

Authors:  R L Hudgin; H Schachter
Journal:  Can J Biochem       Date:  1972-09

5.  Structural studies on human erythrocyte glycoproteins. Alkali-labile oligosaccharides.

Authors:  D B Thomas; R J Winzler
Journal:  J Biol Chem       Date:  1969-11-10       Impact factor: 5.157

6.  Structure of sulfatides biosynthesized in vitro.

Authors:  P Stoffyn; A Stoffyn; G Hauser
Journal:  J Lipid Res       Date:  1971-05       Impact factor: 5.922

7.  Structure of the disaccharide chain of galactosyl-N-acetylgalactosaminyl-protein synthesized in vitro.

Authors:  D H van den Eijnden; R A Barneveld; W E Schiphorst
Journal:  Eur J Biochem       Date:  1979-04

8.  Purification to homogeneity and enzymatic characterization of an alpha-N-acetylgalactosaminide alpha 2 leads to 6 sialyltransferase from porcine submaxillary glands.

Authors:  J E Sadler; J I Rearick; R L Hill
Journal:  J Biol Chem       Date:  1979-07-10       Impact factor: 5.157

9.  Purification to homogeneity of a beta-galactoside alpha2 leads to 3 sialyltransferase and partial purification of an alpha-N-acetylgalactosaminide alpha2 leads to 6 sialyltransferase from porcine submaxillary glands.

Authors:  J E Sadler; J I Rearick; J C Paulson; R L Hill
Journal:  J Biol Chem       Date:  1979-06-10       Impact factor: 5.157

10.  Detection of beta-galactosyl(1 leads to 4)N-acetylglucosaminide alpha(2 leads to 3)-sialyltransferase activity in fetal calf liver and other tissues.

Authors:  D H Van den Eijnden; W E Schiphorst
Journal:  J Biol Chem       Date:  1981-04-10       Impact factor: 5.157
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  3 in total

1.  Sialyltransferase activity in FR3T3 cells transformed with ras oncogene: decreased CMP-Neu5Ac:Gal beta 1-3GalNAc alpha-2,3-sialyltransferase.

Authors:  P Delannoy; H Pelczar; V Vandamme; A Verbert
Journal:  Glycoconj J       Date:  1993-02       Impact factor: 2.916

2.  Bovine colostrum CMP-NeuAc:Gal beta(1-->4)GlcNAc-R alpha(2-->6)-sialyltransferase is involved in the synthesis of the terminal NeuAc alpha(2-->6)GalNAc beta(1-->4)GlcNAc sequence occurring on N-linked glycans of bovine milk glycoproteins.

Authors:  M Nemansky; D H Van den Eijnden
Journal:  Biochem J       Date:  1992-10-01       Impact factor: 3.857

3.  Different reactivity of two brain sialyltransferases towards sulfhydryl reagents. Evidence for a thiol group involved in the nucleotide-sugar binding site of the NeuAc alpha 2-3Gal beta 1-3GalNAc alpha(2-6)sialyltransferase.

Authors:  H Baubichon-Cortay; P Broquet; P George; P Louisot
Journal:  Glycoconj J       Date:  1989       Impact factor: 2.916
  3 in total

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