Literature DB >> 8358231

Sialyltransferase activity in FR3T3 cells transformed with ras oncogene: decreased CMP-Neu5Ac:Gal beta 1-3GalNAc alpha-2,3-sialyltransferase.

P Delannoy1, H Pelczar, V Vandamme, A Verbert.   

Abstract

We have investigated the activity of CMP-Neu5Ac:Gal beta 1-3GalNAc alpha-2,3-sialyltransferase (EC 2.4.99.4) in FR3T3 cells transformed by the Ha-ras oncogene in which we have previously demonstrated the higher expression of the beta-galactosidase alpha-2,6-sialyltransferase (EC 2.4.99.1) [21]. We demonstrate that the presence of the activated ras gene decreases the activity of this specific alpha-2,3-sialyltransferase fourfold. According to the kinetic parameters and to mixing experiments, we can assume that this decreased enzymatic activity reflects a decrease in the number of active O-glycan alpha-2,3-sialyltransferase polypeptides in ras-transformed cells. However, no change in the binding of Peanut agglutinin was observed on the cell surface of ras-transformed FR3T3 suggesting that no change in the sialylation of O-glycan core 1 appeared in these cells, although the activity of the alpha-2,3-sialyltransferase was decreased.

Entities:  

Mesh：

Substances：

Year:  1993        PMID： 8358231     DOI： 10.1007/bf00731192

Source DB:  PubMed          Journal:  Glycoconj J        ISSN： 0282-0080            Impact factor:   2.916


  43 in total

1.  The c-Ha-ras oncogene induces increased expression of beta-galactoside alpha-2, 6-sialyltransferase in rat fibroblast (FR3T3) cells.

Authors:  N Le Marer; V Laudet; E C Svensson; H Cazlaris; B Van Hille; C Lagrou; D Stehelin; J Montreuil; A Verbert; P Delannoy
Journal:  Glycobiology       Date:  1992-02       Impact factor: 4.313

2.  Tissue-specific expression of beta-galactoside alpha-2,6-sialyltransferase. Transcript heterogeneity predicts a divergent polypeptide.

Authors:  T P O'Hanlon; K M Lau; X C Wang; J T Lau
Journal:  J Biol Chem       Date:  1989-10-15       Impact factor: 5.157

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

4.  High-pressure liquid chromatography of sialic acid-containing oligosaccharides.

Authors:  M L Bergh; P Koppen; D H van den Eijnden
Journal:  Carbohydr Res       Date:  1981-08-01       Impact factor: 2.104

5.  Comparative study of the oligosaccharides released from baby hamster kidney cells and their polyoma transformant by hydrazinolysis.

Authors:  K Yamashita; T Ohkura; Y Tachibana; S Takasaki; A Kobata
Journal:  J Biol Chem       Date:  1984-09-10       Impact factor: 5.157

6.  Enzymatic characterization of beta D-galactoside alpha2 leads to 3 sialyltransferase from porcine submaxillary gland.

Authors:  J I Rearick; J E Sadler; J C Paulson; R L Hill
Journal:  J Biol Chem       Date:  1979-06-10       Impact factor: 5.157

7.  Purification to homogeneity of a beta-galactoside alpha2 leads to 3 sialyltransferase and partial purification of an alpha-N-acetylgalactosaminide alpha2 leads to 6 sialyltransferase from porcine submaxillary glands.

Authors:  J E Sadler; J I Rearick; J C Paulson; R L Hill
Journal:  J Biol Chem       Date:  1979-06-10       Impact factor: 5.157

8.  Regulated expression of alpha 2,6-sialyltransferase by the liver-enriched transcription factors HNF-1, DBP, and LAP.

Authors:  E C Svensson; P B Conley; J C Paulson
Journal:  J Biol Chem       Date:  1992-02-15       Impact factor: 5.157

9.  Purification and enzymatic characterization of CMP-sialic acid: beta-galactosyl1----3-N-acetylgalactosaminide alpha 2----3-sialyltransferase from human placenta.

Authors:  D H Joziasse; M L Bergh; H G ter Hart; P L Koppen; G J Hooghwinkel; D H Van den Eijnden
Journal:  J Biol Chem       Date:  1985-04-25       Impact factor: 5.157

10.  N-linked oligosaccharide changes with oncogenic transformation require sialylation of multiantennae.

Authors:  U V Santer; R DeSantis; K J Hård; J A van Kuik; J F Vliegenthart; B Won; M C Glick
Journal:  Eur J Biochem       Date:  1989-04-15
View more
  7 in total

1.  Benzyl-N-acetyl-alpha-D-galactosaminide inhibits the sialylation and the secretion of mucins by a mucin secreting HT-29 cell subpopulation.

Authors:  P Delannoy; I Kim; N Emery; C De Bolos; A Verbert; P Degand; G Huet
Journal:  Glycoconj J       Date:  1996-10       Impact factor: 2.916

Review 2.  Regulation of N-acetylglucosaminyltransferase V and Asn-linked oligosaccharide beta(1,6) branching by a growth factor signaling pathway and effects on cell adhesion and metastatic potential.

Authors:  M Pierce; P Buckhaults; L Chen; N Fregien
Journal:  Glycoconj J       Date:  1997-08       Impact factor: 2.916

3.  Study of O-sialylation of glycoproteins in C6 glioma cells treated with retinoic acid.

Authors:  P Reboul; P George; D Miquel; P Louisot; P Broquet
Journal:  Glycoconj J       Date:  1996-02       Impact factor: 2.916

4.  Purification and characterization of a novel beta-D-galactosides-specific lectin from Clitoria ternatea.

Authors:  Aabgeena Naeem; Shabirul Haque; Rizwan Hasan Khan
Journal:  Protein J       Date:  2007-09       Impact factor: 2.371

5.  Direct demonstration of increased expression of Thomsen-Friedenreich (TF) antigen in colonic adenocarcinoma and ulcerative colitis mucin and its concealment in normal mucin.

Authors:  B J Campbell; I A Finnie; E F Hounsell; J M Rhodes
Journal:  J Clin Invest       Date:  1995-02       Impact factor: 14.808

Review 6.  Selectin Ligands Sialyl-Lewis a and Sialyl-Lewis x in Gastrointestinal Cancers.

Authors:  Marco Trinchera; Adele Aronica; Fabio Dall'Olio
Journal:  Biology (Basel)       Date:  2017-02-23

7.  GalNAc-alpha-O-benzyl inhibits NeuAcalpha2-3 glycosylation and blocks the intracellular transport of apical glycoproteins and mucus in differentiated HT-29 cells.

Authors:  G Huet; S Hennebicq-Reig; C de Bolos; F Ulloa; T Lesuffleur; A Barbat; V Carrière; I Kim; F X Real; P Delannoy; A Zweibaum
Journal:  J Cell Biol       Date:  1998-06-15       Impact factor: 10.539
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.