Literature DB >> 7061403

Genetic transformation of Rhizobium leguminosarum by plasmid DNA.

G S Bullerjahn, R H Benzinger.   

Abstract

We demonstrated the genetic transformation of Rhizobium leguminosarum by R68.45 plasmid DNA by freezing and thawing cell suspensions in the presence of R68.45 plasmid DNA and 20 mM MgCl2. Clones resistant to kanamycin and tetracycline were recovered at a frequency of 10(-8) per recipient cell. No colonies that were doubly drug resistant were recovered in parallel control experiments.

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Year:  1982        PMID： 7061403      PMCID： PMC220134          DOI： 10.1128/jb.150.1.421-424.1982

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  15 in total

1.  GENETIC TRANSFORMATION OF RHIZOBIUM: A REVIEW OF THE WORK OF R. BALASSA.

Authors:  G Balassa
Journal:  Bacteriol Rev       Date:  1963-06

2.  Eukaryotic DNA fragments which act as promoters for a plasmid gene.

Authors:  R L Neve; R W West; R L Rodriguez
Journal:  Nature       Date:  1979-01-25       Impact factor: 49.962

3.  A simple method for the preparation of large quantities of pure plasmid DNA.

Authors:  G O Humphreys; G A Willshaw; E S Anderson
Journal:  Biochim Biophys Acta       Date:  1975-04-02

4.  Properties of plasmids constructed by the in vitro insertion of DNA from Rhizobium leguminosarum or Proteus mirabilis into RP4.

Authors:  A E Jacob; J M Cresswell; R W Hedges; J N Coetzee; J E Beringer
Journal:  Mol Gen Genet       Date:  1976-09-23

5.  A rapid boiling method for the preparation of bacterial plasmids.

Authors:  D S Holmes; M Quigley
Journal:  Anal Biochem       Date:  1981-06       Impact factor: 3.365

6.  Tryptophan genes in Rhizobium--their organization and their transfer to other bacterial genera.

Authors:  A W Johnston; M J Bibb; J E Beringer
Journal:  Mol Gen Genet       Date:  1978-10-24

7.  Transformation and physical properties of R-factor RP4 transferred from Escherichia coli to Rhizobium trifolii.

Authors:  F O'Gara; L K Dunican
Journal:  J Bacteriol       Date:  1973-12       Impact factor: 3.490

8.  Transfection and transformation of Agrobacterium tumefaciens.

Authors:  M Holsters; D de Waele; A Depicker; E Messens; M van Montagu; J Schell
Journal:  Mol Gen Genet       Date:  1978-07-11

9.  Genetic transformation in Rhizobium trifolii.

Authors:  D Drozańska; Z Lorkiewicz
Journal:  Acta Microbiol Pol       Date:  1978

10.  Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.

Authors:  G Ditta; S Stanfield; D Corbin; D R Helinski
Journal:  Proc Natl Acad Sci U S A       Date:  1980-12       Impact factor: 11.205
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  2 in total

1.  Expression of beta-galactosidase controlled by a nitrogenase promoter in stem nodules of Aeschynomene scabra.

Authors:  R P Legocki; A C Yun; A A Szalay
Journal:  Proc Natl Acad Sci U S A       Date:  1984-09       Impact factor: 11.205

2.  Hierarchical analysis of linkage disequilibrium in Rhizobium populations: evidence for sex?

Authors:  V Souza; T T Nguyen; R R Hudson; D Piñero; R E Lenski
Journal:  Proc Natl Acad Sci U S A       Date:  1992-09-01       Impact factor: 11.205
  2 in total

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