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Literature DB >> 355847

Transfection and transformation of Agrobacterium tumefaciens.

M Holsters, D de Waele, A Depicker, E Messens, M van Montagu, J Schell.

Abstract

The freeze thaw transfection procedure of Dityatkin et al. (1972) was adapted for the transfection and transformation of A. tumefaciens. Transfection of the strains B6S3 and B6-6 with DNA of the temperate phage PS8cc186 yielded a maximum frequency of 2 10(-7) transfectants per total recipient population. In transformation of the strain GV3100 with the P type plasmid RP4 a maximum frequency of 3.5 10(-7) transformants per total recipient population was obtained. Agrobacterium Ti-plasmids were introduced in the strain GV3100 with a maximal efficiency of 4.5 10(-8). These experiments provide further evidence that the Ti-plasmid is responsible for the oncogenic properties of A tumefaciens and for its capacity to induce "opine" synthesis in Crown-gall plant cells.

Entities: Species

Mesh：

Year: 1978 PMID： 355847 DOI： 10.1007/bf00267408

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

27 in total

1. Agrobacterium tumefaciens Conn. II. Production of an antibiotic substance.

Authors: T STONIER
Journal: J Bacteriol Date: 1960-06 Impact factor: 3.490

2. General method for the isolation of plasmid deoxyribonucleic acid.

Authors: P Guerry; D J LeBlanc; S Falkow
Journal: J Bacteriol Date: 1973-11 Impact factor: 3.490

3. The interaction of closed circular DNA with intercalative dyes. I. The superhelix density of SV40 DNA in the presence and absence of dye.

Authors: W Bauer; J Vinograd
Journal: J Mol Biol Date: 1968-04-14 Impact factor: 5.469

4. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.

Authors: D B Clewell; D R Helinski
Journal: Proc Natl Acad Sci U S A Date: 1969-04 Impact factor: 11.205

5. A simple method for the preparation of large quantities of pure plasmid DNA.

Authors: G O Humphreys; G A Willshaw; E S Anderson
Journal: Biochim Biophys Acta Date: 1975-04-02

6. Agrocin 84 sensitivity: a plasmid determined property in Agrobacterium tumefaciens.

Authors: G Engler; M Holsters; M Van Montagu; J Schell; J P Hernalsteens
Journal: Mol Gen Genet Date: 1975-07-10

7. Properties of an R factor from Pseudomonas aeruginosa.

Authors: N Datta; R W Hedges; E J Shaw; R B Sykes; M H Richmond
Journal: J Bacteriol Date: 1971-12 Impact factor: 3.490

8. RP4 promotion of transfer of a large Agrobacterium plasmid which confers virulence.

Authors: M D Chilton; S K Farrand; R Levin; E W Nester
Journal: Genetics Date: 1976-08 Impact factor: 4.562

9. Utilization of octopine and nopaline by Agrobacterium.

Authors: J A Lippincott; R Beiderbeck; B B Lippincott
Journal: J Bacteriol Date: 1973-10 Impact factor: 3.490

10. Genetic transformation in Escherichia coli K12.

Authors: S D Cosloy; M Oishi
Journal: Proc Natl Acad Sci U S A Date: 1973-01 Impact factor: 11.205

327 in total

1. A novel promoter from soybean that is active in a complex developmental pattern with and without its proximal 650 base pairs.

Authors: M V Strömvik; V P Sundararaman; L O Vodkin
Journal: Plant Mol Biol Date: 1999-09 Impact factor: 4.076

2. A mini binary vector series for plant transformation.

Authors: C Xiang; P Han; I Lutziger; K Wang; D J Oliver
Journal: Plant Mol Biol Date: 1999-07 Impact factor: 4.076

3. Light modulation of Rubisco in Arabidopsis requires a capacity for redox regulation of the larger Rubisco activase isoform.

Authors: Ning Zhang; Russell P Kallis; Robert G Ewy; Archie R Portis
Journal: Proc Natl Acad Sci U S A Date: 2002-02-19 Impact factor: 11.205

Transfection and transformation of Agrobacterium tumefaciens.

1. Agrobacterium tumefaciens Conn. II. Production of an antibiotic substance.

2. General method for the isolation of plasmid deoxyribonucleic acid.

3. The interaction of closed circular DNA with intercalative dyes. I. The superhelix density of SV40 DNA in the presence and absence of dye.

4. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.

5. A simple method for the preparation of large quantities of pure plasmid DNA.

6. Agrocin 84 sensitivity: a plasmid determined property in Agrobacterium tumefaciens.

7. Properties of an R factor from Pseudomonas aeruginosa.

8. RP4 promotion of transfer of a large Agrobacterium plasmid which confers virulence.

9. Utilization of octopine and nopaline by Agrobacterium.

10. Genetic transformation in Escherichia coli K12.

1. A novel promoter from soybean that is active in a complex developmental pattern with and without its proximal 650 base pairs.

2. A mini binary vector series for plant transformation.

3. Light modulation of Rubisco in Arabidopsis requires a capacity for redox regulation of the larger Rubisco activase isoform.

4. The calcium rhythms of different cell types oscillate with different circadian phases.

5. Laccase down-regulation causes alterations in phenolic metabolism and cell wall structure in poplar.

6. Cloning a peanut resveratrol synthase gene and its expression in purple sweet potato.

7. Characterization of conjugal transfer functions of Agrobacterium tumefaciens Ti plasmid pTiC58.

8. Heterologous expression of Arabidopsis ABF4 gene in potato enhances tuberization through ABA-GA crosstalk regulation.

9. Expression of an expansin gene is correlated with root elongation in soybean.

10. Regulation of Ti plasmid virulence genes by a chromosomal locus of Agrobacterium tumefaciens.