Literature DB >> 7048242

Comparison of an HPLC method with a RIA, EMIT and FIA method for the assay of serum gentamicin with extensive statistical evaluation.

W Hospes, R J Boskma, J R Brouwers.   

Abstract

A high performance liquid chromatography (HPLC) assay, a radioimmunoassay (RIA), an enzyme immunoassay (EMIT) and a fluorescence immunoassay (FIA) method for assaying serum gentamicin were compared in terms of accuracy and precision. Spiked human serum samples in the range of 0-11.6 mg.1(-1) gentamicin were used in all the tests. Regression analysis and analysis of variance were performed. The Performance Index (PI) was used to qualify the different methods. Other aspects of performance were also compared: simplicity, speed, cost, application in relation to workload. After extensive statistical evaluation the FIA method gives the best results.

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Year:  1982        PMID： 7048242     DOI： 10.1007/bf01963658

Source DB:  PubMed          Journal:  Pharm Weekbl Sci        ISSN： 0167-6555


  14 in total

1.  Clinical biostatistics. XXXVII. Demeaned errors, confidence games, nonplussed minuses, inefficient coefficients, and other statistical disruptions of scientific communication.

Authors:  A R Feinstein
Journal:  Clin Pharmacol Ther       Date:  1976-11       Impact factor: 6.875

2.  Enzyme immunoassay for gentamicin.

Authors:  J C Standefer; G C Saunders
Journal:  Clin Chem       Date:  1978-11       Impact factor: 8.327

3.  Homogeneous reactant-labeled fluorescent immunoassay for therapeutic drugs exemplified by gentamicin determination in human serum.

Authors:  J F Burd; R C Wong; J E Feeney; R J Carrico; R C Boguslaski
Journal:  Clin Chem       Date:  1977-08       Impact factor: 8.327

4.  Use and interpretation of common statistical tests in method-comparison studies.

Authors:  J O Westgard; M R Hunt
Journal:  Clin Chem       Date:  1973-01       Impact factor: 8.327

Review 5.  High-pressure liquid chromatography for quantitation of antimicrobial agents.

Authors:  T T Yoshikawa; S K Maitra; M C Schotz; L B Guze
Journal:  Rev Infect Dis       Date:  1980 Mar-Apr

6.  Serum aminoglycoside assay by enzyme-mediated immunoassay (EMIT): correlation with radioimmunoassay, microbiological assays.

Authors:  L O White; L M Scammell; D S Reeves
Journal:  Antimicrob Agents Chemother       Date:  1981-06       Impact factor: 5.191

7.  Micro determination of gentamicin in serum by high-performance liquid chromatography with ultraviolet detection.

Authors:  D M Barends; J S van der Sandt; A Hulshoff
Journal:  J Chromatogr       Date:  1980-05-09

8.  Gentamicin assay by enzymatic adenylylation and the application of a double osmotic shock procedure to prepare gentamicin adenine mono-nucleotide transferase.

Authors:  J Dankert; J Woltjes
Journal:  Zentralbl Bakteriol Orig A       Date:  1977-09

9.  Comparison of gentamicin bioassay values utilizing three different indicator organisms.

Authors:  D J Flournoy; T C Fung; O M Van Duyn
Journal:  Methods Find Exp Clin Pharmacol       Date:  1981 Mar-Apr

10.  High-pressure liquid chromatography analysis and single-dose disposition of tobramycin in human volunteers.

Authors:  D B Haughey; D M Janicke; M Adelman; J J Schentag
Journal:  Antimicrob Agents Chemother       Date:  1980-04       Impact factor: 5.191
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  3 in total

1.  Evaluation of five immunological methods for the assay of serum netilmicin.

Authors:  C Van Remmerden; J R Brouwers; J A Berk; R J Boskma
Journal:  Pharm Weekbl Sci       Date:  1984-04-27

2.  The determination of aminoglycoside antibiotics in serum: a comparison of a high performance liquid chromatographic method with a microbiological assay.

Authors:  D M Barends; A Rutgers; B Van Klingeren; A Hulshoff
Journal:  Pharm Weekbl Sci       Date:  1982-08-20

3.  Analytical accuracy of determinations of aminoglycoside concentrations by enzyme multiplied immunoassay, fluorescence polarization immunoassay, and radioimmunoassay in the presence of heparin.

Authors:  M E O'Connell; K L Heim; C E Halstenson; G R Matzke
Journal:  J Clin Microbiol       Date:  1984-12       Impact factor: 5.948
  3 in total

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