Literature DB >> 7380912

Micro determination of gentamicin in serum by high-performance liquid chromatography with ultraviolet detection.

D M Barends, J S van der Sandt, A Hulshoff.   

Abstract

A procedure for the high-performance liquid chromatographic determination of gentamicin in serum is described using pre-column derivatisation and UV detection. The serum proteins are precipitated with acetonitrile and the gentamicin components in the supernatant are derivatized with 1-fluoro-2,4-dinitrobenzene. The reaction products are chromatographed on a microparticulate C18 reversed-phase column and detected at 365 nm. Sample volumes of 50 microliters are sufficient for the determination of gentamicin concentrations in, and well below, the therapeutic range.

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Year:  1980        PMID： 7380912     DOI： 10.1016/s0378-4347(00)81624-2

Source DB:  PubMed          Journal:  J Chromatogr


  6 in total

1.  Comparison of an HPLC method with a RIA, EMIT and FIA method for the assay of serum gentamicin with extensive statistical evaluation.

Authors:  W Hospes; R J Boskma; J R Brouwers
Journal:  Pharm Weekbl Sci       Date:  1982-04-23

2.  The determination of aminoglycoside antibiotics in serum: a comparison of a high performance liquid chromatographic method with a microbiological assay.

Authors:  D M Barends; A Rutgers; B Van Klingeren; A Hulshoff
Journal:  Pharm Weekbl Sci       Date:  1982-08-20

3.  Development of a high-performance liquid chromatographic assay for G418 sulfate (Geneticin).

Authors:  C Bethune; T Bui; M L Liu; M A Kay; R J Ho
Journal:  Antimicrob Agents Chemother       Date:  1997-03       Impact factor: 5.191

4.  Eradication of mucoid Pseudomonas aeruginosa with fluid liposome-encapsulated tobramycin in an animal model of chronic pulmonary infection.

Authors:  C Beaulac; S Clément-Major; J Hawari; J Lagacé
Journal:  Antimicrob Agents Chemother       Date:  1996-03       Impact factor: 5.191

5.  Pulmonary retention of free and liposome-encapsulated tobramycin after intratracheal administration in uninfected rats and rats infected with Pseudomonas aeruginosa.

Authors:  A Omri; C Beaulac; M Bouhajib; S Montplaisir; M Sharkawi; J Lagacé
Journal:  Antimicrob Agents Chemother       Date:  1994-05       Impact factor: 5.191

Review 6.  Bioanalysis of aminoglycosides using high-performance liquid chromatography.

Authors:  Seth K Amponsah; Joseph A Boadu; Daniel K Dwamena; Kwabena F M Opuni
Journal:  ADMET DMPK       Date:  2022-02-14
  6 in total

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