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Literature DB >> 7047555

Detection of La Crosse arbovirus antigen in mosquito pools: application of chromogenic and fluorogenic enzyme immunoassay systems.

S W Hildreth, B J Beaty, J M Meegan, C L Frazier, R E Shope.

Abstract

An enzyme immunoassay producing either a chromogenic or fluorogenic end product was developed and evaluated for detecting La Crosse viral antigen within mosquito pools. The enzyme immunoassay was found to be sensitive, detecting one infected mosquito within a pool of 100 mosquitoes, and specific, distinguishing between closely related California group viruses. Assays were completed within 5 h after the addition of test samples. La Crosse viral antigen could be readily detected in mosquito pools after seven freeze-thaw cycles.

Entities: Disease

Mesh：

Substances：

Year: 1982 PMID： 7047555 PMCID： PMC272207 DOI： 10.1128/jcm.15.5.879-884.1982

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

11 in total

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Authors: C L Frazier; R E Shope
Journal: J Clin Microbiol Date: 1979-10 Impact factor: 5.948

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Authors: S Pantuwatana; W H Thompson; D M Watts; R P Hanson
Journal: Am J Trop Med Hyg Date: 1972-07 Impact factor: 2.345

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Authors: F A Murphy
Journal: Prog Med Virol Date: 1979

7. Detection of attograms of antigen by a high-sensitivity enzyme-linked immunoabsorbent assay (HS-ELISA) using a fluorogenic substrate.

Authors: A Shalev; A H Greenberg; P J McAlpine
Journal: J Immunol Methods Date: 1980 Impact factor: 2.303

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Authors: F Parc; S Chanteau; E Chungue
Journal: Ann Microbiol (Paris) Date: 1979-10

9. Enzyme-linked immunosorbent assay (ELISA): a practical tool for rapid diagnosis of viruses and other infectious agents.

Authors: R H Yolken
Journal: Yale J Biol Med Date: 1980 Jan-Feb

10. Arbovirus-related encephalitis.

Authors: R E Shope
Journal: Yale J Biol Med Date: 1980 Jan-Feb

15 in total

1. Detection of West Nile virus antigen in mosquitoes and avian tissues by a monoclonal antibody-based capture enzyme immunoassay.

Authors: Ann R Hunt; Roy A Hall; Amy J Kerst; Roger S Nasci; Harry M Savage; Nicholas A Panella; Kristy L Gottfried; Kristen L Burkhalter; John T Roehrig
Journal: J Clin Microbiol Date: 2002-06 Impact factor: 5.948

2. Detection of alphaviruses in a genus-specific antigen capture enzyme immunoassay using monoclonal antibodies.

Authors: I M Greiser-Wilke; V Moennig; O R Kaaden; R E Shope
Journal: J Clin Microbiol Date: 1991-01 Impact factor: 5.948

3. Detecting bunyaviruses of the Bunyamwera and California serogroups by a PCR technique.

Authors: G Kuno; C J Mitchell; G J Chang; G C Smith
Journal: J Clin Microbiol Date: 1996-05 Impact factor: 5.948

4. Enzyme-linked immunosorbent assay typing of California serogroup viruses isolated in Canada.

Authors: H Artsob; L P Spence; C Th'ng
Journal: J Clin Microbiol Date: 1984-08 Impact factor: 5.948

5. Detection of dengue virus type 2 in Aedes albopictus by nucleic acid hybridization with strand-specific RNA probes.

Authors: K Olson; C Blair; R Padmanabhan; B Beaty
Journal: J Clin Microbiol Date: 1988-03 Impact factor: 5.948

6. Economic comparison of enzyme immunoassay and virus isolation procedures for surveillance of arboviruses in mosquito populations.

Authors: S W Hildreth; B J Beaty
Journal: J Clin Microbiol Date: 1987-06 Impact factor: 5.948

7. Stability of St. Louis encephalitis viral antigen detected by enzyme immunoassay in infected mosquitoes.

Authors: T F Tsai; C M Happ; R A Bolin; M Montoya; E Campos; D B Francy; R A Hawkes; J T Roehrig
Journal: J Clin Microbiol Date: 1988-12 Impact factor: 5.948

8. Reverse transcription-PCR detection of LaCrosse virus in mosquitoes and comparison with enzyme immunoassay and virus isolation.

Authors: L P Wasieloski; A Rayms-Keller; L A Curtis; C D Blair; B J Beaty
Journal: J Clin Microbiol Date: 1994-09 Impact factor: 5.948

9. Comparison of enzyme-linked immunosorbent assay with enzyme-linked fluorescence assay with automated readers for detection of rubella virus antibody and herpes simplex virus.

Authors: I C Shekarchi; J L Sever; L Nerurkar; D Fuccillo
Journal: J Clin Microbiol Date: 1985-01 Impact factor: 5.948

10. Sensitive enzyme immunoassay for detecting immunoglobulin M antibodies to Sindbis virus and further evidence that Pogosta disease is caused by a western equine encephalitis complex virus.

Authors: C H Calisher; O Meurman; M Brummer-Korvenkontio; P E Halonen; D J Muth
Journal: J Clin Microbiol Date: 1985-10 Impact factor: 5.948