Literature DB >> 1847149

Detection of alphaviruses in a genus-specific antigen capture enzyme immunoassay using monoclonal antibodies.

I M Greiser-Wilke1, V Moennig, O R Kaaden, R E Shope.   

Abstract

A genus-specific antigen capture assay using similar combinations of monoclonal antibodies for capture and detection of 24 alphaviruses belonging to the seven serocomplexes was developed. The sensitivity of the test ranged from 10(3.4) 50% tissue culture infective doses/ml for o'nyong-nyong virus to 10(6.1) 50% tissue culture infective doses/ml for Middelburg virus. The antigen capture test uses a combination of cross-reacting monoclonal antibodies directed against the nucleocapsid protein and envelope glycoprotein E1 of Semliki Forest virus.

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Year:  1991        PMID: 1847149      PMCID: PMC269716          DOI: 10.1128/jcm.29.1.131-137.1991

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN: 0095-1137            Impact factor:   5.948


  27 in total

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Authors:  J CASALS
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Journal:  J Virol       Date:  1973-11       Impact factor: 5.103

4.  Radioimmune precipitation of group A arboviruses.

Authors:  J M Dalrymple; A Y Teramoto; R D Cardiff; P K Russell
Journal:  J Immunol       Date:  1972-09       Impact factor: 5.422

5.  Detection of La Crosse arbovirus antigen in mosquito pools: application of chromogenic and fluorogenic enzyme immunoassay systems.

Authors:  S W Hildreth; B J Beaty; J M Meegan; C L Frazier; R E Shope
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6.  Most alphaviruses share a conserved epitopic region on their nucleocapsid protein.

Authors:  I Greiser-Wilke; V Moenning; O R Kaaden; L T Figueiredo
Journal:  J Gen Virol       Date:  1989-03       Impact factor: 3.891

7.  Detection of eastern equine encephalomyelitis viral antigen in avian blood by enzyme immunoassay: a laboratory study.

Authors:  T W Scott; J G Olson
Journal:  Am J Trop Med Hyg       Date:  1986-05       Impact factor: 2.345

8.  Periodate or glutaraldehyde for preparing peroxidase conjugates?

Authors:  D M Boorsma; J G Streefkerk
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9.  Purification and composition of the proteins from Sindbis virus grown in chick and BHK cells.

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Journal:  J Virol       Date:  1976-12       Impact factor: 5.103

10.  Reevaluation of the western equine encephalitis antigenic complex of alphaviruses (family Togaviridae) as determined by neutralization tests.

Authors:  C H Calisher; N Karabatsos; J S Lazuick; T P Monath; K L Wolff
Journal:  Am J Trop Med Hyg       Date:  1988-03       Impact factor: 2.345

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  8 in total

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2.  Microbial inactivation for safe and rapid diagnostics of infectious samples.

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Journal:  Indian J Med Res       Date:  2013       Impact factor: 2.375

4.  Isolation and characterisation of a human-like antibody fragment (scFv) that inactivates VEEV in vitro and in vivo.

Authors:  Torsten Rülker; Luzie Voß; Philippe Thullier; Lyn M O' Brien; Thibaut Pelat; Stuart D Perkins; Claudia Langermann; Thomas Schirrmann; Stefan Dübel; Hans-Jürgen Marschall; Michael Hust; Birgit Hülseweh
Journal:  PLoS One       Date:  2012-05-30       Impact factor: 3.240

5.  Global emergence of Alphaviruses that cause arthritis in humans.

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6.  Development of a quantitative competitive reverse transcription polymerase chain reaction (QC-RT-PCR) for detection and quantitation of Chikungunya virus.

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7.  Development of human antibody fragments using antibody phage display for the detection and diagnosis of Venezuelan equine encephalitis virus (VEEV).

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8.  Human-like antibodies neutralizing Western equine encephalitis virus.

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  8 in total

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