Anomalous preferences of cultured macrophages for hydrophobic and roughened substrata.

Peritoneal macrophages were plated out on a series of artificial haptotactic substrata consisting of grid patterns of vacuum-evaporated palladium metal alternating with hydrophobic untreated polystyrene and with hydrophilic sulphonated polystyrene. By their active locomotion the macrophages accumulated preferentially onto the less hydrophilic of either pair of these alternative substrata. This order of substratum preference is precisely the opposite to that shown by fibroblastic cells. Macrophages were also found to accumulate preferentially onto roughened culture surfaces as opposed to smooth ones, which is again opposite to the behaviour of fibroblasts. These opposite substratum preferences shown by macrophages may reflect physical as well as functional differences in their surfaces, and could serve as assay criteria for macrophages and cell types putatively equivalent to macrophages.