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Literature DB >> 7029525

Regulation of DNA replication: "target" determinant of the replication control elements of plasmid R6-5 lies within a control element gene.

H Danbara, G Brady, J K Timmis, K N Timmis.

Abstract

The replication control system of plasmid R6-5 has been investigated by characterization of high-copy-number mutant miniplasmids, development of an in vivo assay for the site of action or "target" of the replication control elements, and sequence analysis of the replication control regions of the wild-type plasmid and two copy-number mutant derivatives. These and other experiments have shown that three plasmid determinants--copA/incA, copB, and copT--are involved in DNA replication control. The products of the copB and copA/incA genes, a 9500-dalton basic polypeptide and either a 7200-dalton basic polypeptide or a short untranslated RNA molecule, respectively, are negative-acting elements that interact with the third element, their target, the copT DNA sequence, or its product to regulate the frequency of initiation of plasmid replication. The location of copT within the copA/incA gene and 1600 base pairs upstream from the origin of replication indicates that regulation is effected at a preinitiation stage of replication, such as the production of a primer or other initiation factor.

Entities: Gene

Mesh：

Year: 1981 PMID： 7029525 PMCID： PMC320230 DOI： 10.1073/pnas.78.8.4699

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

33 in total

1. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.

Authors: A C Chang; S N Cohen
Journal: J Bacteriol Date: 1978-06 Impact factor: 3.490

2. Isolation, by tetracycline selection, of small plasmids derived from R-factor R12 in Escherichia coli K-12.

Authors: S Mickel; W Bauer
Journal: J Bacteriol Date: 1976-07 Impact factor: 3.490

3. A new method for sequencing DNA.

Authors: A M Maxam; W Gilbert
Journal: Proc Natl Acad Sci U S A Date: 1977-02 Impact factor: 11.205

4. Isolation and characterization of the minimal fragment required for autonomous replication ("basic replicon") of a copy mutant (pKN102) of the antibiotic resistance factor R1.

Authors: R Kollek; W Oertel; W Goebel
Journal: Mol Gen Genet Date: 1978-06-01

5. Origin and direction of replication of the drug resistance plasmid R100.1 and of a resistance transfer factor derivative in synchronized cultures.

Authors: L Silver; M Chandler; E B de la Tour; L Caro
Journal: J Bacteriol Date: 1977-09 Impact factor: 3.490

6. Plasmid incompatibility and control of replication: copy mutants of the R-factor R1 in Escherichia coli K-12.

Authors: B E Uhlin; K Nordström
Journal: J Bacteriol Date: 1975-11 Impact factor: 3.490

7. Replication control in a composite plasmid constructed by in vitro linkage of two distinct replicons.

Authors: F Cabello; K Timmis; S N Cohen
Journal: Nature Date: 1976-01-29 Impact factor: 49.962

8. Cloning and characterization of EcoRI and HindIII restriction endonuclease-generated fragments of antibiotic resistance plasmids R6-5 and R6.

Authors: K N Timmis; F Cabello; S N Cohen
Journal: Mol Gen Genet Date: 1978-06-14

Review 6. Regulation of plasmid replication.

Authors: J R Scott
Journal: Microbiol Rev Date: 1984-03

7. Replication control mutations of plasmid R6-5 and their effects on interactions of the RNA-I control element with its target.

Authors: G Brady; J Frey; H Danbara; K N Timmis
Journal: J Bacteriol Date: 1983-04 Impact factor: 3.490

Regulation of DNA replication: "target" determinant of the replication control elements of plasmid R6-5 lies within a control element gene.

1. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.

2. Isolation, by tetracycline selection, of small plasmids derived from R-factor R12 in Escherichia coli K-12.

3. A new method for sequencing DNA.

4. Isolation and characterization of the minimal fragment required for autonomous replication ("basic replicon") of a copy mutant (pKN102) of the antibiotic resistance factor R1.

5. Origin and direction of replication of the drug resistance plasmid R100.1 and of a resistance transfer factor derivative in synchronized cultures.

6. Plasmid incompatibility and control of replication: copy mutants of the R-factor R1 in Escherichia coli K-12.

7. Replication control in a composite plasmid constructed by in vitro linkage of two distinct replicons.

8. Cloning and characterization of EcoRI and HindIII restriction endonuclease-generated fragments of antibiotic resistance plasmids R6-5 and R6.

9. Amino acid sequence of Cro regulatory protein of bacteriophage lambda.

10. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.

1. Interaction between the antisense and target RNAs involved in the regulation of IncB plasmid replication.

2. Mechanism of binding of the antisense and target RNAs involved in the regulation of IncB plasmid replication.

3. Characterization of the gene products produced in minicells by pSM1, a derivative of R100.

4. Copy mutant of mini-Rts1: lowered binding affinity of mutated RepA protein to direct repeats.

5. Copy mutants of plasmid R1: effects of base pair substitutions in the copA gene on the replication control system.

Review 6. Regulation of plasmid replication.

7. Replication control mutations of plasmid R6-5 and their effects on interactions of the RNA-I control element with its target.

8. The incompatibility product of IncFII R plasmid NR1 controls gene expression in the plasmid replication region.

9. The sites of action of the two copy number control functions of plasmid R1.

10. Incompatibility mutants of IncFII plasmid NR1 and their effect on replication control.