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Literature DB >> 353525

Isolation and characterization of the minimal fragment required for autonomous replication ("basic replicon") of a copy mutant (pKN102) of the antibiotic resistance factor R1.

Abstract

The mini plasmids deriving from pKN102, a copy mutant of the antibiotic resistance factor R1drd-19 of E. coli, share a common DNA sequence of 2.6 kb, which carries the minimal functions for autonomous replication. By cloning of two PstI fragments of this region it could be demonstrated that the "basic replicon" is a DNA segment not larger than 1.8 kb, which carries the orgin of replication and the genetic information for at least two proteins. Protein F (NW=11.000 dalton) seems to be synthesed in larger amounts in minicells of E. coli than protein C (20.000). Plasmids containing this isolated replicon of R1 are completely compatible with the parental plasmid R1drd-19.

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Year: 1978 PMID： 353525 DOI： 10.1007/bf00333850

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

16 in total

1. Cloning, isolation, and characterization of replication regions of complex plasmid genomes.

Authors: K Timmis; F Cabello; S N Cohen
Journal: Proc Natl Acad Sci U S A Date: 1975-06 Impact factor: 11.205

2. Two origins of replication in composite R plasmid DNA.

Authors: D Perlman; R H Rownd
Journal: Nature Date: 1976-01-29 Impact factor: 49.962

3. Site specific recombination involved in the generation of small plasmids.

Authors: E Ohtsubo; M Rosenbloom; H Schrempf; W Goebel; J Rosen
Journal: Mol Gen Genet Date: 1978-02-16

4. Transposition and insertion of intact, deleted and enlarged ampicillin transposon Tn3 from mini-R1 (Rsc) plasmids into transfer factors.

Authors: W Goebel; W Lindenmaier; F Pfeifer; H Schrempf; B Schelle
Journal: Mol Gen Genet Date: 1977-11-29

5. Characterization of small ampicillin resistance plasmids (Rsc) originating from the mutant antibiotic resistance factor R1drd-19B2.

Authors: G Luibrand; D Blohm; H Mayer; W Goebel
Journal: Mol Gen Genet Date: 1977-03-28

6. Construction of biologically functional bacterial plasmids in vitro.

Authors: S N Cohen; A C Chang; H W Boyer; R B Helling
Journal: Proc Natl Acad Sci U S A Date: 1973-11 Impact factor: 11.205

7. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.

Authors: W M Bonner; R A Laskey
Journal: Eur J Biochem Date: 1974-07-01

8. Plasmid incompatibility and control of replication: copy mutants of the R-factor R1 in Escherichia coli K-12.

Authors: B E Uhlin; K Nordström
Journal: J Bacteriol Date: 1975-11 Impact factor: 3.490

9. Mutations in R factors of Escherichia coli causing an increased number of R-factor copies per chromosome.

Authors: K Nordström; L C Ingram; A Lundbäck
Journal: J Bacteriol Date: 1972-05 Impact factor: 3.490

10. R factor proteins synthesized in Escherichia coli minicells: incorporation studies with different R factors and detection of deoxyribonucleic acid-binding proteins.

Authors: S B Levy
Journal: J Bacteriol Date: 1974-12 Impact factor: 3.490

38 in total

Review 1. Replication and control of circular bacterial plasmids.

Authors: G del Solar; R Giraldo; M J Ruiz-Echevarría; M Espinosa; R Díaz-Orejas
Journal: Microbiol Mol Biol Rev Date: 1998-06 Impact factor: 11.056

2. Recombinant plasmids capable to replication in B. subtilis and E. coli.

Authors: J Kreft; K Bernhard; W Goebel
Journal: Mol Gen Genet Date: 1978-06-01

3. Isolation and characterization of a conditional replication mutant of the antibiotic resistance factor R1 affected in the gene of the replication protein repA.

Authors: S Ortega; G de Torrontegui; R Díaz
Journal: Mol Gen Genet Date: 1989-05

4. Genetic analysis of the inter-relationship between plasmid replication and incompatibility.

Authors: P A Meacock; S N Cohen
Journal: Mol Gen Genet Date: 1979-07-13

5. Structural and functional analysis of cloned DNA segments containing the replication and incompatibility regions of a miniplasmid derived from a copy number mutant of NR1.

Authors: D P Taylor; S N Cohen
Journal: J Bacteriol Date: 1979-01 Impact factor: 3.490

6. The nucleotide sequence of a DNA fragment from the replication origin of the antibiotic resistance factor R1drd19.

Authors: W Oertel; R Kollek; E Beck; W Goebel
Journal: Mol Gen Genet Date: 1979-03-27

Review 7. Plasmid incompatibility.

Authors: R P Novick
Journal: Microbiol Rev Date: 1987-12

8. Plasmid replication functions. II. Cloning analysis of the repA replication region of antibiotic resistance plasmid R6-5.

Authors: I Andrés; P M Slocombe; F Cabello; J K Timmis; R Lurz; H J Burkardt; K N Timmis
Journal: Mol Gen Genet Date: 1979-01-05

9. Clustering of genes involved in replication, copy number control, incompatibility, and stable maintenance of the resistance plasmid R1drd-19.

Authors: S Molin; P Stougaard; B E Uhlin; P Gustafsson; K Nordström
Journal: J Bacteriol Date: 1979-04 Impact factor: 3.490

10. Plasmid replication functions: two distinct segments of plasmid R1, RepA and RepD, express incompatibility and are capable of autonomous replication.

Authors: H Danbara; J K Timmis; R Lurz; K N Timmis
Journal: J Bacteriol Date: 1980-12 Impact factor: 3.490