| Literature DB >> 7029470 |
B R Amirikyan, A V Vologodskii.
Abstract
The paper presents measurements of the difference in the melting temperature of a colE1 DNA region when it is located inside the DNA helix and at its end. A direct comparison of calculations based on the rigorous theory of helix-coil transition with experimental data for .2 M Na+ (the conditions for fully reversible melting) yielded the value of 2.5-5x10(-5) for the cooperatively factor sigma. We discuss the reversibility of DNA melting and the possibility of applying the "all-or-nothing" concept to the melting of DNA regions.Mesh:
Substances:
Year: 1981 PMID: 7029470 PMCID: PMC327533 DOI: 10.1093/nar/9.20.5469
Source DB: PubMed Journal: Nucleic Acids Res ISSN: 0305-1048 Impact factor: 16.971