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Literature DB >> 6253910

Equilibrium melting of plasmid ColE1 DNA: electron-microscopic visualization.

A S Borovik, Y A Kalambet, Y L Lyubchenko, V T Shitov, E I Golovanov.

Abstract

The fine structure of the melting curve for the linear colE1 DNA has been obtained. To find the ColE1 DNA regions corresponding to peaks in the melting curve's fine structure, we fixed the melted DNA regions with glyoxal /12/. Electron-microscopic denaturation maps were obtained for nine temperature points within the melting range. Thereby the whole process of colE1 DNA melting was reconstructed in detail. Spectrophotometric and electron microscopic data were used for mapping the distribution of Gc-pairs over the DNA molecule. The most AT-rich DNA regions (28 and 37% of GC-pairs), 380 and 660 bp long resp., are located on both sides of the site of ColE1 DNA's cleavage by EcoR1 endonuclease. The equilibrium denaturation maps are compared with maps obtained by the method of Inman /20/ for eight points of the kinetic curve of ColE1 DNA unwinding by formaldehyde.

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Year: 1980 PMID： 6253910 PMCID： PMC324227 DOI： 10.1093/nar/8.18.4165

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

15 in total

1. High resolution thermal denaturation of DNA: thermalites of bacteriophage DNA.

Authors: D L Vizard; A T Ansevin
Journal: Biochemistry Date: 1976-02-24 Impact factor: 3.162

2. Fine structure of DNA melting curves.

Authors: Y L Lyubchenko; M D Frank-Kamenetskii; A V Vologodskii; Y S Lazurkin; G G Gause
Journal: Biopolymers Date: 1976-06 Impact factor: 2.505

3. Fine structures in denaturation curves of bacteriophage lambda DNA. Their relation to the intramolecular heterogeneity in base compositon.

Authors: S Yabuki; O Gotoh; A Wada
Journal: Biochim Biophys Acta Date: 1975-07-07

4. A rapid procedure for purification of EcoRI endonuclease.

Authors: J Sümegi; D Breedveld; P Hossenlopp; P Chambon
Journal: Biochem Biophys Res Commun Date: 1977-05-09 Impact factor: 3.575

5. Denaturation map of bacteriophage T7 DNA and direction of DNA transcription.

Authors: B Gómez; D Lang
Journal: J Mol Biol Date: 1972-09-28 Impact factor: 5.469

6. Hysteresis and partial irreversibility of denaturation of DNA as a means of investigating the topology of base distribution constraints: application to a yeast rho- (petite) mitochondrial DNA.

Authors: F Michel
Journal: J Mol Biol Date: 1974-10-25 Impact factor: 5.469

Equilibrium melting of plasmid ColE1 DNA: electron-microscopic visualization.

1. High resolution thermal denaturation of DNA: thermalites of bacteriophage DNA.

2. Fine structure of DNA melting curves.

3. Fine structures in denaturation curves of bacteriophage lambda DNA. Their relation to the intramolecular heterogeneity in base compositon.

4. A rapid procedure for purification of EcoRI endonuclease.

5. Denaturation map of bacteriophage T7 DNA and direction of DNA transcription.

6. Hysteresis and partial irreversibility of denaturation of DNA as a means of investigating the topology of base distribution constraints: application to a yeast rho- (petite) mitochondrial DNA.

7. Base compisition heterogeneity in kinetoplast DNA FROM FOUR SPECIES OF HEMOFLAGELLATES.

8. The reaction of glyoxal with nucleic acid components. V. Denaturation of DNA under the action of glyoxal.

9. Location of the cooperative melting regions in bacteriophage fd DNA.

10. Specific fragmentation of T7 phage DNA at low-melting sites.

1. DNA overstretching in the presence of glyoxal: structural evidence of force-induced DNA melting.

Review 2. Imaging of DNA and Protein-DNA Complexes with Atomic Force Microscopy.

3. Energy-structure correlations of plasmid DNA in different topological forms.

4. The effect of nucleotide substitution on DNA denaturation profiles.

5. A comparison of experimental and theoretical melting maps for replicative form of phi X174 DNA.

6. The nonequilibrium character of DNA melting: effects of the heating rate on the fine structure of melting curves.

7. Localization of low-melting regions in phage T7 DNA.

8. Determination of DNA cooperativity factor.

Review 9. Optical tweezers experiments resolve distinct modes of DNA-protein binding.

10. Gel electrophoresis of partially denatured DNA. Retardation effect: its analysis and application.